concanavalin-a and Carcinoma--Transitional-Cell

We studied the lectin binding patterns of 40 initial superficial and 10 subsequent invasive bladder tumors by the avidin-biotin-peroxidase complex (ABC) method using the following biotin-labeled lectins: PNA, DBA, UEA-I, BS-I, ConA and WGA. We observed the relationship between lectin binding and subsequent course of initial superficial tumors, grade and stage (T). DBA or WGA staining tumors and Con A negative tumors revealed no recurrence or superficial recurrence. Low grade tumors were DBA or BS-I positive and high grade tumors were ConA positive. Low staging tumors possessed DBA or WGA positiveness and high staging tumors had ConA positiveness. From these results we considered that negative staining of WGA or DBA, or positive staining of ConA was a change accompanying the malignant potentiality.

Topics: Adult; Aged; Aged, 80 and over; Carcinoma, Transitional Cell; Concanavalin A; Female; Humans; Lectins; Male; Middle Aged; Peanut Agglutinin; Plant Lectins; Urinary Bladder Neoplasms; Wheat Germ Agglutinins

Binding sites for lectins of peanuts, soya-beans and concanavalin A were identified in the tissues of normal human urinary bladder and its transitional cell tumors (31 patients) using the lectin-peroxidase technique. No binding sites for any of the lectins were found in normal urothelium. Receptors for peanut and soya-bean lectins were observed on the plasma membranes of 20% of tumor cells in transitional cell papillomas. A diffuse distribution of those lectins in tumor cell cytoplasm was interpreted as a tendency to malignant transformation of tumor. There were no concanavalin A receptors in tumor cells.

Topics: Acetylgalactosamine; Adult; Aged; Aged, 80 and over; Carcinoma, Transitional Cell; Concanavalin A; Galactose; Histocytochemistry; Humans; Lectins; Male; Mannose; Middle Aged; Papilloma; Peanut Agglutinin; Plant Lectins; Receptors, Mitogen; Soybean Proteins; Urinary Bladder; Urinary Bladder Neoplasms

Topics: Administration, Topical; Animals; Antineoplastic Agents; Bleomycin; Carcinoma, Transitional Cell; Concanavalin A; HeLa Cells; Mice; Urinary Bladder Neoplasms

Longitudinal experiments were performed in which lymphocytes from patients were compared with simultaneously studied normals. Patient lymphocytes were examined for their ability to function as stimulator and responder cells in a one-way MLC. Additionally, lymphocyte mitogenic response to PHA and Con A were examined. We observed changes in these parameters that could be correlated with the clinical course of the patients.

Topics: Carcinoma, Transitional Cell; Concanavalin A; Humans; In Vitro Techniques; Lymphocyte Activation; Lymphocyte Culture Test, Mixed; Lymphocytes; Male; Middle Aged; Phytohemagglutinins; Time Factors; Urinary Bladder Neoplasms

Patients with transitional cell carcinoma of the bladder have a highly significant stepwise decrease in responsiveness to challenge with DNCB with advancing stage of disease. Seventy-five percent of those patients with superficial tumors are skin-test positive versus only 35% for those having tumors that are locally advanced and/or metastatic. MLC response and ability to stimulate in this culture as well as PHA and Con A response of blood leukocytes have been studied in relation to stage of disease and therapy. Recent irradiation appears to inhibit significantly MLC responsiveness, and PHA and Con A blastogenesis. Although responsiveness for this group of patients is decreased from normal, a further decrease occurs in responsiveness to Con A with advancing stage of disease. Blood leukocytes from some patients with urinary bladder carcinoma appear to have a decreased ability to function as stimulator cells in one-way MLC. This ability to stimulate returns to normal levels with tumor removal.

Topics: Carcinoma, Transitional Cell; Concanavalin A; Dinitrochlorobenzene; Humans; In Vitro Techniques; Lymphocyte Activation; Lymphocyte Culture Test, Mixed; Lymphocytes; Male; Phytohemagglutinins; Skin Tests; Urinary Bladder Neoplasms