concanamycin-a and Disease-Models--Animal

Peroxisome proliferator activated receptor alpha (PPARα), a regulator of enzymes involved in β oxidation, has been reported to influence lymphocyte activation. The purpose of this study was to determine whether PPARα plays a role in T cell-mediated hepatitis induced by Concanavalin A (ConA).. Taken together, these data suggest that PPARα within the liver plays an important role in ConA-mediated liver injury through regulation of NKT cell recruitment and/or survival.

Topics: Animals; Cytokines; Disease Models, Animal; Enzyme-Linked Immunosorbent Assay; Galactosylceramides; Hepatitis, Autoimmune; Immunohistochemistry; Macrolides; Male; Mice; Mice, Inbred C57BL; PPAR alpha; Real-Time Polymerase Chain Reaction; T-Lymphocytes

Dengue virus (DENV) infection in neuronal cells was speculated to trigger neuropathy. Herein, we determined the blockade of DENV infection by targeting endocytic pathways in vitro and in vivo. In DENV-infected mouse brains, we previously showed that viral proteins are expressed in neuronal cells around the hippocampus with accompanying neurotoxicity. DENV caused infection, including entry, double-stranded (ds)RNA replication, protein expression, and virus release, followed by cytotoxicity in the mouse neuronal Neuro-2a cell line. Pharmacologically blocking clathrin-mediated endocytosis of the DENV retarded viral replication. Targeting vacuolar-type H

Topics: Animals; Antifungal Agents; Cell Line; Cell Survival; Clathrin; Dengue; Dengue Virus; Disease Models, Animal; Endocytosis; Humans; In Vitro Techniques; Macrolides; Mice; Neurons; Virus Internalization; Virus Replication