colistin and Dermatitis--Contact

Colistin methanesulfonate sodium (CLMS) is a widely-used antibiotic. To our best knowledge, only two cases of contact allergy to CLMS have been reported. We described a 4-year-old girl with contact dermatitis evoked by CLMS that had been applied as an ophthalmic solution. The dermatitis started after administration for 21 days. The diagnosis was confirmed by as is and constituent patch tests. We reviewed the literature in which contact allergy due to CLMS and colistin sulfate is described.

Topics: Anti-Bacterial Agents; Child, Preschool; Colistin; Dermatitis, Contact; Drug Eruptions; Eye Infections; Eye, Artificial; Facial Dermatoses; Female; Humans; Ophthalmic Solutions; Patch Tests

A confirmatory method for the determination of colistin in animal tissues, egg, milk, and feed was developed and validated. Colistin A and colistin B were extracted from samples with the mixture of 10% trichloroacetic acid-acetonitrile and isolated with mixed-mode weak cation exchange cartridge. Analytes were separated from matrix components using ultra-high performance liquid chromatography, and detected with electrospray ionization on a triple quadrupole mass spectrometer. Mean recoveries ranged from 78.0% to 115.6% with intra-day and inter-day relative standard deviation lower than 8.4% and 12.4%, respectively. The quantitation limits for different matrices were between 5 and 30 μg/kg, which was satisfactory for surveillance monitoring. The developed method was applied to the analysis of real samples collected from different provinces of China, and 19 out of 348 samples were found to be contaminated, with the highest concentration of approximately 12,000 μg/kg colistin A and 10,000 μg/kg colistin B in feed.

Topics: Animal Feed; Animals; Anti-Bacterial Agents; China; Chromatography, High Pressure Liquid; Colistin; Dermatitis, Contact; Eggs; Food Analysis; Food Contamination; Milk; Reproducibility of Results; Tandem Mass Spectrometry

Topics: Allopurinol; Ampicillin; Aspirin; Cell Migration Inhibition; Colistin; Dermatitis, Contact; Drug Eruptions; Hemagglutination Tests; Humans; Immunity, Cellular; Immunoglobulin E; Leukocytes; Lymphocyte Activation; Penicillins; Phenobarbital; Quinine; Skin Tests; Sulfonamides; Tetracycline