colfosceril-palmitate and Liver-Neoplasms

We studied the effect of zoledronic acid encapsulated into liposomes (L-ZOL) on tumorassociated macrophages in the stroma of hepatocellular carcinoma xenograft. Liposomes were prepared from 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, 1,2-dipalmitoyl-snglycero-3-phospho-sn-1-glycerol, and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[carboxy(polyethylene glycol)-2000] using thin film method and loaded with zoledronic acid. It was shown that L-ZOL promoted apoptosis of RAW264.7 cells, eliminate much more protumoral M2 macrophages than antitumoral M1 macrophages in the tumor xenograft, and did not significantly reduce the size of xenograft in 6 days. Thus, the effect of treatment depends on the ratio between antitumoral M1 and protumoral M2 polarized macrophages in the tumor.

Topics: 1,2-Dipalmitoylphosphatidylcholine; Animals; Antineoplastic Agents; Apoptosis; Carcinoma, Hepatocellular; Cell Differentiation; Cell Line, Tumor; Cell Lineage; Drug Compounding; Female; Humans; Liposomes; Liver Neoplasms; Mice; Mice, Inbred BALB C; Phosphatidylethanolamines; Phosphatidylglycerols; Polyethylene Glycols; RAW 264.7 Cells; Tumor Burden; Tumor Microenvironment; Tumor-Associated Macrophages; Xenograft Model Antitumor Assays; Zoledronic Acid