coenzyme-q10 and Uterine-Cervical-Neoplasms

Coenzyme Q10 is an antioxidant that may have a therapeutic role in cervical cancer.. We investigated the cellular and molecular effects of 30 micromol/L Coenzyme Q10 in HeLa cells. Cell growth assays, fluorescence-activated cell sorting analyses, and Oil Red O staining were performed. Microarray experiments were performed in duplicate and analyzed on the basis of 2-fold changes in levels of gene expression.. Coenzyme Q10 inhibited cell growth and led to apoptosis. Microarray analysis showed that 264 sequences were altered over time, with enrichment in lipid-related genes. Enhanced lipid accumulation was confirmed with Oil Red O staining.. A lipid response to Coenzyme Q10 may affect mechanisms of growth inhibition in HeLa cells.

Topics: Antioxidants; Apoptosis; Cell Division; Coenzymes; Female; Gene Expression Regulation, Neoplastic; HeLa Cells; Humans; Lipid Peroxidation; Oxidation-Reduction; Polymerase Chain Reaction; Reference Values; RNA, Messenger; Sensitivity and Specificity; Transcriptional Activation; Ubiquinone; Uterine Cervical Neoplasms

Cervical intraepithelial neoplasia (CIN) may, at times, unpredictably progress to invasive carcinoma of the cervix. Epidemiological nutritional studies suggest that higher dietary consumption and circulating levels of certain micronutrients may be protective against cervical cancer. However, a preventive role of dietary antioxidants in CIN is not well established. The purpose of this cross-sectional study was to investigate the comparative plasma concentrations of three potent antioxidants, coenzyme Q(10,) alpha-tocopherol and gamma-tocopherol, in women with normal Pap smears and patients with a biopsy-confirmed histopathological lesion diagnosed as CIN or cervical cancer. Plasma concentrations of coenzyme Q(10,) alpha-tocopherol and gamma-tocopherol were measured by high-pressure liquid chromatography in both normal women without any history of abnormal Pap smears (n=48), and patients with histopathologically confirmed diagnoses of: (a) CIN I, n=98; (b) CIN II, n=49; (c) CIN III, n=10; and (d) cervical cancer, n=25. The mean plasma levels of coenzyme Q(10), alpha-tocopherol and gamma-tocopherol were significantly lower (P<0.001,<0.001, and<0.001, respectively by Kruskal-Wallis test) in patients with various grades of CIN and cervical cancer compared with controls. After controlling for age and smoking, an inverse association between histological grades of epithelial lesions and both plasma coenzyme Q(10) and alpha-tocopherol concentrations was observed. The low plasma concentrations of coenzyme Q(10) may be due to deficient dietary intake or a decrease in endogenous coenzyme Q(10) biosynthesis that may reflect increased utilization as a result of free radical reactive oxygen species induced oxidative stress. Further molecular studies on the mechanistic role of antioxidants in women with precancer cervical lesions are needed.

Topics: Adolescent; Adult; alpha-Tocopherol; Antioxidants; Coenzymes; Cross-Sectional Studies; Diet; Female; gamma-Tocopherol; Humans; Middle Aged; Regression Analysis; Ubiquinone; Uterine Cervical Dysplasia; Uterine Cervical Neoplasms