coenzyme-q10 and Swine-Diseases

The aim of this study was to evaluate the effect of low-energy laser irradiation, coenzyme Q10 and vitamin E supplementation on the apoptosis of macrophages and muscle precursor cells during skeletal muscle regeneration after bupivacaine-induced injury. The experiment was conducted on 75 gilts, divided into 5 experimental groups: I--control, II--low-energy laser irradiation, III--coenzyme Q10, IV--coenzyme Q10 and vitamin E, V--vitamin E. Muscle necrosis was induced by injection of 0.5% bupivacaine hydrochloride. The animals were euthanized on subsequent days after injury. Samples were formalin fixed and processed routinely for histopathology. Apoptosis was detected using the TUNEL method. The obtained results indicate that low-energy laser irradiation has a beneficial effect on macrophages and muscle precursor cell activity during muscle post-injury regeneration and protects these cells against apoptosis. Vitamin E has a slightly lower protective effect, limited mainly to the macrophages. Coenzyme Q10 co-supplemented with vitamin E increases the activity of macrophages and muscle precursor cells, myotube and young muscle formation. Importantly, muscle precursor cells seem to be more sensitive to apoptosis than macrophages in the environment of regenerating damaged muscle.

Topics: Animals; Antioxidants; Apoptosis; Bupivacaine; Dose-Response Relationship, Radiation; Drug Therapy, Combination; Female; Lasers; Muscle, Skeletal; Muscular Diseases; Swine; Swine Diseases; Ubiquinone; Vitamin E

The aim of the study was to observe the effect of coenzyme Q10 and vitamin E supplementation on the course of the regeneration process of the longissimus lumborum muscle after bupivacaine-induced myonecrosis as well as to determine the correlation between the level of those substances in plasma and their levels in damaged and non-damaged muscular tissue in pigs. The obtained results indicate that the course of regeneration of a damaged muscle is affected to a higher extent by coenzyme Q10 than by vitamin E. The administration of coenzyme Q10 and vitamin E has a significant impact on the increase in the level of those substances in damaged muscles and plasma of animals.

Topics: Anesthetics, Local; Animal Feed; Animals; Antioxidants; Bupivacaine; Coenzymes; Female; Muscle, Skeletal; Muscular Diseases; Necrosis; Regeneration; Swine; Swine Diseases; Ubiquinone; Vitamin E