coelenterazine and Melanoma

coelenterazine has been researched along with Melanoma* in 1 studies

Other Studies

1 other study(ies) available for coelenterazine and Melanoma

Article	Year
Stem cell-based photodynamic therapy. Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 2012, Volume: 11, Issue:7 We have transfected murine neural stem cells (NSCs) and rat umbilical cord matrix-derived stem cells (RUCMSCs) with a plasmid expressing gaussia luciferase (gLuc). These cells are engineered to secrete the luciferase. We have used gLuc containing supernatant from culturing the NSCs to perform in vitro photodynamic therapy of murine melanoma cells (B16F10), and RUCMSCs to perform in vivo PDT of lung melanomas in C57BL/6 mice. The treatment system was comprised of aminolevulic acid as a prodrug for the synthesis of the photosensitizer protoporphyrin IX, gaussia luciferase, and its' substrate coelenterazine. A significant reduction of the number of live melanoma cells in vitro and a borderline significant retardation of tumour growth in vivo was observed after coelenterazine-mediated PDT. Topics: Aminolevulinic Acid; Animals; Cell Line, Tumor; Cell Survival; Female; Fetal Blood; Imidazoles; Luciferases; Lung Neoplasms; Melanoma; Mice; Mice, Inbred C57BL; Neural Stem Cells; Oxidation-Reduction; Photochemotherapy; Photosensitizing Agents; Plasmids; Protoporphyrins; Pyrazines; Rats; Stem Cell Transplantation; Stem Cells; Transfection	2012

Article

Year

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 2012, Volume: 11, Issue:7

We have transfected murine neural stem cells (NSCs) and rat umbilical cord matrix-derived stem cells (RUCMSCs) with a plasmid expressing gaussia luciferase (gLuc). These cells are engineered to secrete the luciferase. We have used gLuc containing supernatant from culturing the NSCs to perform in vitro photodynamic therapy of murine melanoma cells (B16F10), and RUCMSCs to perform in vivo PDT of lung melanomas in C57BL/6 mice. The treatment system was comprised of aminolevulic acid as a prodrug for the synthesis of the photosensitizer protoporphyrin IX, gaussia luciferase, and its' substrate coelenterazine. A significant reduction of the number of live melanoma cells in vitro and a borderline significant retardation of tumour growth in vivo was observed after coelenterazine-mediated PDT.

Topics: Aminolevulinic Acid; Animals; Cell Line, Tumor; Cell Survival; Female; Fetal Blood; Imidazoles; Luciferases; Lung Neoplasms; Melanoma; Mice; Mice, Inbred C57BL; Neural Stem Cells; Oxidation-Reduction; Photochemotherapy; Photosensitizing Agents; Plasmids; Protoporphyrins; Pyrazines; Rats; Stem Cell Transplantation; Stem Cells; Transfection

2012