cobrotoxin and Leukemia--T-Cell

cobrotoxin has been researched along with Leukemia--T-Cell* in 1 studies

Other Studies

1 other study(ies) available for cobrotoxin and Leukemia--T-Cell

Article	Year
[Specific killing of human leukemic T-cells by a monoclonal antibody coupled to the cytotoxin from Chinese cobra]. Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae, 1992, Volume: 14, Issue:3 The immunotoxin (IT) was prepared by conjugating cytotoxin from Chinese Cobra (Naja naja Atra) venom with monoclonal antibody (McAb) Wu71 directed to human T-cells. First, calcium ions were used to suppress the cytolytic reaction while cell antigen was allowed to react with McAb. Then magnesium ions and the chelating agent EGTA were used to abolish the calcium inhibition. With this treatment, the IT showed high cytotoxicity for the leukemic cell line CEM, which was antigen positive (82.4% of the cells were killed at a concentration of 0.5 x 10(-6) mol/L of IT), but very little cytotoxicity for the antigen negative cell line Raji (23.3% cells were killed at the same concentration). Under scanning electron microscopy, it could be seen that the cell membranes of CEM were broken by IT, and the cells had died. The results suggest that the McAb Wu71 plus cytotoxin IT has potential application in leukemia therapy. Topics: Antibodies, Monoclonal; Cobra Neurotoxin Proteins; Humans; Immunotoxins; Leukemia, T-Cell; T-Lymphocytes; Tumor Cells, Cultured	1992

Article

Year

[Specific killing of human leukemic T-cells by a monoclonal antibody coupled to the cytotoxin from Chinese cobra].

Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae, 1992, Volume: 14, Issue:3

The immunotoxin (IT) was prepared by conjugating cytotoxin from Chinese Cobra (Naja naja Atra) venom with monoclonal antibody (McAb) Wu71 directed to human T-cells. First, calcium ions were used to suppress the cytolytic reaction while cell antigen was allowed to react with McAb. Then magnesium ions and the chelating agent EGTA were used to abolish the calcium inhibition. With this treatment, the IT showed high cytotoxicity for the leukemic cell line CEM, which was antigen positive (82.4% of the cells were killed at a concentration of 0.5 x 10(-6) mol/L of IT), but very little cytotoxicity for the antigen negative cell line Raji (23.3% cells were killed at the same concentration). Under scanning electron microscopy, it could be seen that the cell membranes of CEM were broken by IT, and the cells had died. The results suggest that the McAb Wu71 plus cytotoxin IT has potential application in leukemia therapy.

Topics: Antibodies, Monoclonal; Cobra Neurotoxin Proteins; Humans; Immunotoxins; Leukemia, T-Cell; T-Lymphocytes; Tumor Cells, Cultured

1992