cobra-cardiotoxin-proteins and Muscular-Dystrophy--Duchenne

cobra-cardiotoxin-proteins has been researched along with Muscular-Dystrophy--Duchenne* in 3 studies

Trials

1 trial(s) available for cobra-cardiotoxin-proteins and Muscular-Dystrophy--Duchenne

ArticleYear
Mitochondrial alterations and oxidative stress in an acute transient mouse model of muscle degeneration: implications for muscular dystrophy and related muscle pathologies.
    The Journal of biological chemistry, 2014, Jan-03, Volume: 289, Issue:1

    Muscular dystrophies (MDs) and inflammatory myopathies (IMs) are debilitating skeletal muscle disorders characterized by common pathological events including myodegeneration and inflammation. However, an experimental model representing both muscle pathologies and displaying most of the distinctive markers has not been characterized. We investigated the cardiotoxin (CTX)-mediated transient acute mouse model of muscle degeneration and compared the cardinal features with human MDs and IMs. The CTX model displayed degeneration, apoptosis, inflammation, loss of sarcolemmal complexes, sarcolemmal disruption, and ultrastructural changes characteristic of human MDs and IMs. Cell death caused by CTX involved calcium influx and mitochondrial damage both in murine C2C12 muscle cells and in mice. Mitochondrial proteomic analysis at the initial phase of degeneration in the model detected lowered expression of 80 mitochondrial proteins including subunits of respiratory complexes, ATP machinery, fatty acid metabolism, and Krebs cycle, which further decreased in expression during the peak degenerative phase. The mass spectrometry (MS) data were supported by enzyme assays, Western blot, and histochemistry. The CTX model also displayed markers of oxidative stress and a lowered glutathione reduced/oxidized ratio (GSH/GSSG) similar to MDs, human myopathies, and neurogenic atrophies. MS analysis identified 6 unique oxidized proteins from Duchenne muscular dystrophy samples (n = 6) (versus controls; n = 6), including two mitochondrial proteins. Interestingly, these mitochondrial proteins were down-regulated in the CTX model thereby linking oxidative stress and mitochondrial dysfunction. We conclude that mitochondrial alterations and oxidative damage significantly contribute to CTX-mediated muscle pathology with implications for human muscle diseases.

    Topics: Adolescent; Adult; Aged; Animals; Cell Line; Child; Child, Preschool; Cobra Cardiotoxin Proteins; Gene Expression Regulation; Humans; Infant; Mice; Middle Aged; Mitochondria, Muscle; Mitochondrial Proteins; Muscular Dystrophy, Animal; Muscular Dystrophy, Duchenne; Myoblasts, Skeletal; Oxidative Stress

2014

Other Studies

2 other study(ies) available for cobra-cardiotoxin-proteins and Muscular-Dystrophy--Duchenne

ArticleYear
Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy.
    The American journal of pathology, 2016, Volume: 186, Issue:5

    Duchenne muscular dystrophy is a lethal X-linked muscle disorder. We have already reported that osteopontin (OPN), an inflammatory cytokine and myogenic factor, is expressed in the early dystrophic phase in canine X-linked muscular dystrophy in Japan, a dystrophic dog model. To further explore the possibility of OPN as a new biomarker for disease activity in Duchenne muscular dystrophy, we monitored serum OPN levels in dystrophic and wild-type dogs at different ages and compared the levels to other serum markers, such as serum creatine kinase, matrix metalloproteinase-9, and tissue inhibitor of metalloproteinase-1. Serum OPN levels in the dystrophic dogs were significantly elevated compared with those in wild-type dogs before and 1 hour after a cesarean section birth and at the age of 3 months. The serum OPN level was significantly correlated with the phenotypic severity of dystrophic dogs at the period corresponding to the onset of muscle weakness, whereas other serum markers including creatine kinase were not. Immunohistologically, OPN was up-regulated in infiltrating macrophages and developmental myosin heavy chain-positive regenerating muscle fibers in the dystrophic dogs, whereas serum OPN was highly elevated. OPN expression was also observed during the synergic muscle regeneration process induced by cardiotoxin injection. In conclusion, OPN is a promising biomarker for muscle regeneration in dystrophic dogs and can be applicable to boys with Duchenne muscular dystrophy.

    Topics: Age Factors; Animals; Biomarkers; Cobra Cardiotoxin Proteins; Diaphragm; Dogs; Male; Matrix Metalloproteinase 9; Muscle, Skeletal; Muscular Dystrophy, Animal; Muscular Dystrophy, Duchenne; Osteopontin; Phenotype; Regeneration; Tissue Inhibitor of Metalloproteinase-1

2016
Analysis of Pax7 expressing myogenic cells in zebrafish muscle development, injury, and models of disease.
    Developmental dynamics : an official publication of the American Association of Anatomists, 2011, Volume: 240, Issue:11

    The transcription factor Pax7 is a marker and regulator of muscle progenitors and satellite cells that contribute to the embryonic development and postembryonic growth of skeletal muscle in vertebrates, as well as to its repair and regeneration. Here, we identify Pax7(+ve) myogenic cells in the zebrafish and characterize their behavior in postembryonic stages. Mononucleate Pax7(+ve) cells can first be found associated with myofibers at 72 hours post fertilization (hpf). To follow the behavior of muscle progenitor cells in vivo, we generated transgenic lines expressing fluorescent proteins under the control of the pax7a or pax3a promoters. We established an injury model using cardiotoxin injection and monitored cell proliferation and myogenic regulatory factor expression in myogenic precursors cells and muscle fibers after injury using proliferation markers and the transgenic lines. We also analyzed Pax7(+ve) cells in animals with dystrophic phenotypes and found an increased number compared with wild-type.

    Topics: Animals; Animals, Genetically Modified; Cobra Cardiotoxin Proteins; Disease Models, Animal; Embryo, Nonmammalian; Gene Expression Regulation, Developmental; Genes, Reporter; Humans; Muscle Development; Muscle, Skeletal; Muscular Diseases; Muscular Dystrophy, Duchenne; Myoblasts; PAX7 Transcription Factor; Zebrafish

2011