clove and Zika-Virus-Infection

To estimate the prevalence of IgG antibodies against six arboviruses in people living with HIV-1 (PLWHIV) in Madagascar, we tested samples collected between January 2018 and June 2021. We used a Luminex-based serological assay to detect IgG antibodies against antigens from Dengue virus serotypes 1-4 (DENV1-4), Zika virus (ZIKV), West Nile virus (WNV), Usutu virus (USUV), Chikungunya virus (CHIKV), and O'nyong nyong virus (ONNV). Of the 1036 samples tested, IgG antibody prevalence was highest for ONNV (28.4%), CHIKV (26.7%), WNV-NS1 (27.1%), DENV1 (12.4%), USUV (9.9%), and DENV3 (8.9%). ZIKV (4.9%), DENV2 (4.6%), WNV-D3 (5.1%), and DENV4 (1.4%) were lower. These rates varied by province of origin, with the highest rates observed in Toamasina, on the eastern coast (50.5% and 56.8%, for CHIKV and ONNV, respectively). The seroprevalence increased with age for DENV1 and 3 (

Topics: Antibodies, Viral; Arboviruses; Chikungunya Fever; Chikungunya virus; HIV Infections; Humans; Immunoglobulin G; Madagascar; Seroepidemiologic Studies; West Nile virus; Zika Virus; Zika Virus Infection

It was previously reported that a malaria infection may interfere with the specificity of a commercial ELISA test against Zika virus (ZIKV). We analyzed 1,216 plasma samples from healthy, pregnant women collected in two sites in Madagascar in 2010 for ZIKV antibodies using a commercial ELISA and for Plasmodium infection by PCR. This screen revealed six putative ZIKV-positive samples by ELISA. These results could not be confirmed by indirect immunofluorescence assays or virus neutralization tests. Four of these six samples were also positive for P. falciparum. We noted that the frequency of malaria positivity was higher in ZIKV-ELISA positive samples (50% and 100% in the two study sites) than ZIKV-negative samples (17% and 10%, respectively), suggesting that malaria may have led to false ZIKV-ELISA positives.

Topics: Antibodies, Neutralizing; Antibodies, Viral; Coinfection; Cross-Sectional Studies; Enzyme-Linked Immunosorbent Assay; Female; Geography; Humans; Madagascar; Malaria; Neutralization Tests; Population Surveillance; Pregnancy; Sensitivity and Specificity; Seroepidemiologic Studies; Zika Virus; Zika Virus Infection

Topics: Adult; Americas; Disease Outbreaks; Female; Humans; Influenza A virus; Influenza, Human; Lassa Fever; Madagascar; Male; Nigeria; Plague; Zika Virus Infection