clove and Virus-Diseases

Madagascar is a hotspot of biodiversity, but poverty and population growth provoke a high risk of conflict between food security and biodiversity conservation in this tropical country. Numerous vector-borne diseases, including viral infections, affect public health in Madagascar and a continuous expansion of anthropogenically used areas intensifies contact on the human-wildlife interface. However, data on human and animal pathogens in potential insect vectors is limited. Therefore, we conducted a parasitological and virological survey of 785 adult female mosquitoes between March and May 2016 at the Ankarafantsika National Park in northwestern Madagascar. Screening included Alpha-, Phlebo-, and Flaviviridae and the recently described filarial nematode species, Lemurfilaria lemuris. The predominant mosquito genus was Culex (91%), followed by Mansonia (4.1%), Anopheles (3.4%), and Aedes (0.9%). Viral screening revealed no arboviruses, but an insect-specific flavivirus in two Culex sitiens pools. No pools screened positive for the lemur-specific filarial nematode L. lemuris.

Topics: Aedes; Animals; Anopheles; Biodiversity; Culex; Disease Reservoirs; Filariasis; Flavivirus; Lemur; Madagascar; Mosquito Vectors; Nematoda; Virus Diseases

The increasing use of malaria diagnostic tests reveals a growing proportion of patients with fever but no malaria. Clinicians and health care workers in low-income countries have few tests to diagnose causes of fever other than malaria although several diseases share common symptoms. We propose here to assess etiologies of fever in Madagascar to ultimately improve management of febrile cases.. Consenting febrile outpatients aged 6 months and older were recruited in 21 selected sentinel sites throughout Madagascar from April 2014 to September 2015. Standard clinical examinations were performed, and blood and upper respiratory specimens were taken for rapid diagnostic tests and molecular assays for 36 pathogens of interest for Madagascar in terms of public health, regardless of clinical status.. A total of 682 febrile patients were enrolled. We detected at least one pathogen in 40.5% (276/682) of patients and 6.2% (42/682) with co-infections. Among all tested patients, 26.5% (181/682) had at least one viral infection, 17.0% (116/682) had malaria and 1.0% (7/682) presented a bacterial or a mycobacterial infection. None or very few of the highly prevalent infectious agents in Eastern Africa and Asia were detected in this study, such as zoonotic bacteria or arboviral infections.. These results raise questions about etiologies of fever in Malagasy communities. Nevertheless, we noted that viral infections and malaria still represent a significant proportion of causes of febrile illnesses. Interestingly our study allowed the detection of pathogens of public health interest such as Rift Valley Fever Virus but also the first case of laboratory-confirmed leptospirosis infection in Madagascar.

Topics: Adolescent; Adult; Aged; Bacterial Infections; Child; Child, Preschool; Community Health Centers; Female; Fever; Humans; Madagascar; Malaria; Male; Middle Aged; Prospective Studies; Public Health; Virus Diseases; Young Adult

Introduced animals impact endemic populations through predation, competition, and disease transmission. Populations of endemic carnivores in Madagascar are declining, and pathogens transmitted from introduced species may further endanger these unique species. We assessed the exposure of introduced and endemic carnivores to common viral and parasitic pathogens in two national parks of Madagascar (Kirindy Mitea National Park and Ankarafantsika National Park) and their neighboring villages. We also identified variables associated with the presence of antibodies to these pathogens in fosa ( Cryptoprocta ferox ). Introduced and endemic species were exposed to canine parvovirus, canine herpesvirus, feline calicivirus, and Toxoplasma gondii . Domestic dogs ( Canis familiaris ) and cats ( Felis catus ) may be sources of infection for these pathogens. Prevalence of antibodies to Toxoplasma in captured fosa was >93%, and adults were more likely to be exposed than immature individuals. Our data provide a basis upon which to evaluate and manage risks of pathogen transmission between species.

Topics: Animals; Animals, Wild; Antibodies, Viral; Carnivory; Cats; Dogs; Madagascar; Parasites; Prevalence; Virus Diseases

In Madagascar, very little is known about the etiology and prevalence of acute respiratory infections (ARIs) in a rural tropical area. Recent data are needed to determine the viral and atypical bacterial etiologies in children with defined clinical manifestations of ARIs.. During one year, we conducted a prospective study on ARIs in children between 2 to 59 months in the community hospital of Ampasimanjeva, located in the south-east of Madagascar. Respiratory samples were analyzed by multiplex real-time RT-PCR, including 18 viruses and 2 atypical bacteria. The various episodes of ARI were grouped into four clinical manifestations with well-documented diagnosis: "Community Acquired Pneumonia"(CAP, group I), "Other acute lower respiratory infections (Other ALRIs, group II)", "Upper respiratory tract infections with cough (URTIs with cough, group III)"and "Upper respiratory tract infections without cough (URTIs without cough, group IV)".. 295 children were included in the study between February 2010 and February 2011. Viruses and/or atypical bacteria respiratory pathogens were detected in 74.6% of samples, the rate of co-infection was 27.3%. Human rhinovirus (HRV; 20.5%), metapneumovirus (HMPV A/B, 13.8%), coronaviruses (HCoV, 12.5%), parainfluenza virus (HPIV, 11.8%) and respiratory syncytial virus A and B (RSV A/B, 11.8%) were the most detected. HRV was predominantly single detected (23.8%) in all the clinical groups while HMPV A/B (23.9%) was mainly related to CAP (group I), HPIV (17.3%) to the "Other ALRIs" (group II), RSV A/B (19.5%) predominated in the group "URTIs with cough" (group III) and Adenovirus (HAdV, 17.8%) was mainly detected in the "without cough" (group IV).. This study describes for the first time the etiology of respiratory infections in febrile children under 5 years in a malaria rural area of Madagascar and highlights the role of respiratory viruses in a well clinically defined population of ARIs.

Topics: Acute Disease; Bacteria; Bacterial Infections; Child, Preschool; Coinfection; Community-Acquired Infections; Cough; Fever; Humans; Madagascar; Paramyxoviridae Infections; Polymerase Chain Reaction; Prevalence; Prospective Studies; Respiratory Syncytial Virus Infections; Respiratory Tract Infections; Rural Population; Seasons; Tropical Climate; Virus Diseases; Viruses

Our study included a total of 318 diarrheic stools and 52 normal stools collected from out-patients with acute diarrhea at welfare Center and children admitted at Antananarivo City children's Hospital, or control free of infectious disease during 8 months period. Enzyme linked immunosorbant assay and tissue cultures revealed the presence of 152 viral particles (47%) from children with diarrhea and 29 viral particles (55%) from control children. Positive cases were distributed according age, sex, and season factors. The highest infection rate was found in 25-36 months old of the children with diarrhea (72%). The two sexes were equally infected. Enteroviruses were isolated from diarrheic stools with a high frequency (43%) during the rainy and warm season while Rotaviruses were the prevailing agent during the dry and cool season, and Adenoviruses came in second place (19%). In view of our results, the etiological role of these viruses in diarrhea is discussed.

Topics: Adenoviridae; Adenoviridae Infections; Child, Preschool; Diarrhea, Infantile; Enterovirus; Enterovirus Infections; Feces; Female; Humans; Infant; Infant, Newborn; Madagascar; Male; Rotavirus; Rotavirus Infections; Seasons; Virus Diseases

A total of 80 nasopharyngeal secretions collected from malagasy children (53 boys and 27 girls) with viral acute respiratory infection, aged from 6 days to 10 year old admitted to the Pediatric Department of Antananarivo General Hospital from may to July 1983, were investigated by indirect immunofluorescence method. 54--samples were found positive for respiratory viruses. Distribution according age groups and sex has been studied: children belonging to 25-36 month age group and male sex were more infected. Following viral strains were detected in increasing frequency: Para-influenzae 3: 25 p. 100; RSV: 18 p. 10/; Adenovirus: 18 p. 100; Influenzae A: 13 p. 100; Influenzae B: 9 p. 100; Para-influenzae 1: 5 p. 100.

Topics: Acute Disease; Adenoviruses, Human; Child; Child, Preschool; Female; Fluorescent Antibody Technique; Humans; Infant; Infant, Newborn; Influenza A virus; Influenza B virus; Madagascar; Male; Nasopharynx; Parainfluenza Virus 1, Human; Parainfluenza Virus 3, Human; Respiratory Syncytial Viruses; Respiratory Tract Infections; Sex Factors; Virus Diseases

Topics: Animals; Bacterial Infections; Humans; Madagascar; Protozoan Infections; Rickettsia Infections; Virus Diseases; Zoonoses

Topics: Animals; Dog Diseases; Dogs; Gastroenteritis; Hemagglutination Inhibition Tests; Hemagglutination Tests; Madagascar; Parvoviridae; Virus Diseases

Topics: Animals; Bacterial Infections; Bibliographies as Topic; Lemur; Lemuridae; Madagascar; Parasitic Diseases, Animal; Virus Diseases

Topics: Animals; Bacterial Infections; Disease Models, Animal; Lemur; Lemuridae; Madagascar; Parasitic Diseases; Parasitic Diseases, Animal; Virus Diseases

Topics: Animal Diseases; Animals; Bacterial Infections; Bibliographies as Topic; Biochemical Phenomena; Biochemistry; Entomology; Food; Hematology; Humans; Madagascar; Mycoses; Parasitic Diseases; Pharmacology; Venoms; Virus Diseases