clove and Pleurisy

Some species of Campomanesia are used in the folk medicine due to anti-inflammatory, anti-diarrheal, anti-diabetes and hypercholesterolemic. However studies with Campomanesia guazumifolia (Cambess.) O. Berg. are scarce.. This study investigated the anti-inflammatory activity and toxicological profile of infusion obtained from leaves of Campomanesia guazumifolia in mice.. Leaves infusion of C. guazumifolia was obtained in the proportion of 20 g/L (leaves/water) at 95-100 °C for 10 min in an enclosed container. The acute toxicity of the leaves infusion of C. guazumifolia lyophilized (ICG) was assessed by oral administration to female mice at doses of 500, 1000, 2000, and 5000 mg/kg, and the general behavior and toxic symptoms were observed for 14 days. In the subacute toxicity model, female mice were treated orally with the ICG (250, 500, and 1000 mg/kg) during 28 days, and biochemical, toxic signs and the estrous cycle were evaluated. The anti-inflammatory activity of the ICG (70, 300 and 700 mg/kg) was analyzed using carrageenan-induced pleurisy and inflammatory paw (mechanical and thermal hyperalgesia).. The results evidenced the anti-inflammatory potential with low toxicity of infusion of the leaves of C. guazumifolia, supporting the popular use of this species.

Topics: Animals; Anti-Inflammatory Agents; Carrageenan; Edema; Estrous Cycle; Female; Flavonoids; Hyperalgesia; Male; Mice; Myrtaceae; Plant Extracts; Plant Leaves; Pleurisy; Toxicity Tests, Acute; Toxicity Tests, Subacute

This study evaluates the anti-inflammatory, antihyperalgesic, and antidepressive potential of the hydroalcoholic extract of Campomanesia adamantium fruit barks (CAE) on rodents and determines the safety of this plant.. The acute toxicity of CAE was evaluated by oral administration to female rats as single doses of 0, 500, 1000, or 2000 mg/kg body weight. General behavior and toxic symptoms were observed for 14 days. In the subacute toxicity test, male and female rats received 125 or 250 mg/kg body weight of CAE for 28 days. The oral anti-inflammatory activity of CAE was evaluated in carrageenan-induced pleurisy in male mice. The effect of treatment with CAE (100 mg/kg) for 15 days was evaluated in mechanical hyperalgesia (electronic von Frey), depressive behavior (forced swimming test), and cold hypersensitivity in spared nerve injury (SNI) model in rats.. No clinical signs of toxicity were observed in animals from the experimental groups during acute and subacute exposure to CAE. At pleurisy test, the oral administration of CAE significantly inhibited leukocyte migration and protein leakage at all doses tested when compared to control. Oral administration of CAE for 3-15 days significantly inhibited SNI-induced mechanical hyperalgesia and increased immobility in the forced swim test. Finally, on the 15th day, oral treatment with CAE prevented the increase in sensitivity to a cold stimulus induced by SNI.. The present study shows that C. adamantium extract has anti-inflammatory, antihyperalgesic, and antidepressive properties in rodents without causing toxicity.

Topics: Animals; Anti-Inflammatory Agents, Non-Steroidal; Antidepressive Agents; Brazil; Cold Temperature; Depression; Dietary Supplements; Dose-Response Relationship, Drug; Ethnopharmacology; Female; Fruit; Hyperalgesia; Male; Medicine, Traditional; Myrtaceae; Neuralgia; Plant Bark; Plant Extracts; Pleurisy; Rats, Wistar; Toxicity Tests, Acute; Toxicity Tests, Subacute

Topics: Biopsy; Female; Humans; Madagascar; Male; Middle Aged; Neoplasms; Pleura; Pleurisy; Retrospective Studies