clobetasol and Ear-Diseases

Previous studies have shown the antidotal efficacy of topical iodine at 15 and 30 min post-exposure to sulfur mustard (SM). Here we demonstrate efficacy at longer intervals (20, 30, 45, and 60 min, respectively, for data) using an improved topical povidone-iodine preparation termed N66, which contains steroidal and non-steroidal anti-inflammatory agents. In the mouse, N66 reduced severity of ear edema by 43, 47, 44, and 36%; ear epidermal ulceration by 74, 58, 45, and 58%; and epidermal necrosis by 54, 34, 26, and 31% at the respective time points. A similar effect was observed with encrustation. The healing marker, grade of acanthotic area, showed dramatic increases of 39.6-, 25.3-, 20.9-, and 22-fold. Severity of the dermal parameters, acute inflammation and dermal necrosis, was reduced by 63, 34, 34, and 38% and 80, 54, 54, and 59%, respectively. In guinea pig skin, topical treatment with N66 45 min post-exposure reduced the SM-induced ulceration area by 75%. The histological parameters subepidermal microblister formation, epidermal ulceration, epidermal necrosis, and encrustation were reduced by 63, 61, 41, and 41%, respectively. The healing marker, grade of acanthotic area, was elevated by 73%. N66 induced a statistically significant reduction in two dermal markers for tissue damage: acute inflammation (33%) and dermal necrosis (48%). Reduced skin damage was also observed in areas adjacent the treated sites. The pharmacologically active components of N66 showed additive effect. These findings suggest that the povidone-iodine preparation combined with anti-inflammatory agents functions as a potent antidote against skin lesions induced by SM at relatively long intervals between exposure and treatment.

Topics: Administration, Topical; Animals; Anti-Inflammatory Agents; Chemical Warfare Agents; Clobetasol; Disease Models, Animal; Drug Combinations; Ear Diseases; Edema; Guinea Pigs; Male; Mice; Mice, Inbred ICR; Mustard Gas; Piroxicam; Povidone-Iodine; Protective Agents; Skin; Skin Diseases; Skin Irritancy Tests; Time Factors

Chondrodermatitis nodularis chronica helicis is characterized by the presence of small, intensely painful nodules on the free border of the helix of the ear with unknown origin. The lesion is typically localized unilaterally on the right ear. Pressure and trauma may play a role.

Topics: Cartilage Diseases; Clobetasol; Dermatitis; Ear Cartilage; Ear Diseases; Female; Humans; Middle Aged; Ointments

Clobetasol-17-propionate (CP), a synthetic glucocorticoid (GC), reduced skin thickness in rats. Both the subcutaneous injection and topical applications of RU 486 counteracted CP-induced reduction in skin thickness. Topical application of the CP cream completely inhibited the ear edema produced by croton oil. A less potent GC, hydrocortisone-17-butyrate, also inhibited ear edema. This antiinflammatory effect was not abolished by the subcutaneous injection or topical application of RU 486. These observations suggest that GC-induced skin atrophy is mediated by glucocorticoid receptors (GRs), while the inhibition of croton oil-induced inflammation by GC is primarily related to the direct effects of GC, which are not mediated by GRs. Our findings suggest that RU 486 inhibits the atrophogenic effect of GCs without interfering with their antiinflammatory effect. Dissociation of antiinflammatory and atrophogenic activity of GC seems favorable in treating inflammatory skin diseases lacking epidermal proliferation.

Topics: Animals; Anti-Inflammatory Agents; Atrophy; Clobetasol; Croton Oil; Dexamethasone; Ear Diseases; Edema; Glucocorticoids; Ligands; Male; Mifepristone; Rats; Rats, Wistar; Receptors, Glucocorticoid; Skin