clay and Hemorrhage

Uncontrollable bleeding from military conflicts, accidents, and surgical procedures is a major life-threatening factor. Rapid, safe, and convenient hemostasis is critical to the survival of bleeding patients in prehospital care. However, the peel-off of hemostats such as kaolinite sheets from the cotton fibers often poses a risk of distal thrombosis. Here, an efficient clay hemostat of halloysite nanotubes is tightly bound onto commercial cotton fibers, which is capillary mediated by biopolymer alginate with Ca

Topics: Alginates; Bandages; Biopolymers; Clay; Cotton Fiber; Hemorrhage; Hemostasis; Hemostatics; Humans; Nanotubes

Uncontrolled massive hemorrhage is a crucial cause of death, and developing efficient hemostatic materials are of great medical importance. Herein, we prepared a halloysite-chitosan-collagen composite sponge by directional freeze-drying method and coated the sponge with hydrophobic polydimethylsiloxane coating for rapid and effective hemostasis. The aligned channel structure of the sponge with a pore size of ~30 μm was beneficial for the transport of blood. Morphology and spectrum results suggested that chitosan and collagen are capable of adsorbing on the outer surface of HNTs due to the hydrogen bonding and electrostatic attractions. The directional freeze-dried sponge absorbed the majority of the blood within 10 s, and that process essentially was completed in 30 s, which are faster than its non-directional counterpart. The composite sponges exhibited high antibacterial properties towards E. coli and S. aureus, and they are non-cytotoxic towards mouse fibroblasts and have high hemocompatibility. The hemostatic dressing avoided unnecessary blood loss because of excessive blood absorption. In vivo experiments of rats also confirmed the ability of the asymmetric sponges to rapidly clot and reduce reducing blood loss. This work developed a high-performance and hemostatic dressing by material design and processing technique, which shows a promising application in wound healing.

Topics: Animals; Anti-Bacterial Agents; Bandages; Chitosan; Clay; Collagen; Escherichia coli; Hemorrhage; Hemostasis; Hemostatics; Mice; Rats; Staphylococcus aureus

Uncontrolled hemorrhage from trauma or surgery can lead to death. In this study, chitosan/kaolin (CSK) and chitosan/montmorillonite (CSMMT) composites were prepared from chitosan (CS), kaolin (K), and montmorillonite (MMT) as raw materials to control bleeding. The physiochemical properties and surface morphology of CSK and CSMMT composites were analyzed by Fourier transform infrared spectrometry (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), zeta potentials, and X-ray fluorescence (XRF). The hemostatic mechanism was measured in vitro by activated partial thromboplastin time (APTT), prothrombin time (PT), in vitro clotting time, erythrocyte aggregation, and thromboelastogram (TEG). The hemostasis ability was further verified by using tail amputation and arteriovenous injury models in rats. The biocompatibility of CSK and CSMMT was evaluated by in vitro hemolysis, cytotoxicity assays, as well as acute toxicity test and skin irritation tests. The results show that CSK and CSMMT are promising composite materials with excellent biocompatibility and hemostatic properties that can effectively control bleeding.

Topics: Animals; Bentonite; Chitosan; Clay; Hemorrhage; Hemostatics; Kaolin; Rats; Spectroscopy, Fourier Transform Infrared

Death from acute hemorrhage is a major problem in military conflicts, traffic accidents, and surgical procedures, et al. Achieving rapid effective hemostasis for pre-hospital care is essential to save lives in massive bleeding. An ideal hemostasis material should have those features such as safe, efficient, convenient, economical, which remains challenging and most of them cannot be achieved at the same time. In this work, we report a rapid effective nanoclay-based hemostatic membranes with nanoclay particles incorporate into polyvinylpyrrolidone (PVP) electrospun fibers. The nanoclay electrospun membrane (NEM) with 60 wt% kaolinite (KEM1.5) shows better and faster hemostatic performance in vitro and in vivo with good biocompatibility compared with most other NEMs and clay-based hemostats, benefiting from its enriched hemostatic functional sites, robust fluffy framework, and hydrophilic surface. The robust hemostatic bandages based on nanoclay electrospun membrane is an effective candidate hemostat in practical application.

Topics: Animals; Bandages; Clay; Disease Models, Animal; Hemorrhage; Hemostasis; Hemostatics; Humans; Kaolin; Liver; Male; Nanostructures; Povidone; Rats; Rats, Sprague-Dawley; Spleen; Surgical Wound

In recent years, a wide variety of research has been carried out in the field of novel technologies to stop severe bleeding. In several studies, coagulation properties of minerals such as zeolite, bentonite and halloysite have been proven. In this study, the effect of a new impregnated sterile gauze containing bentonite and halloysite minerals was studied on blood coagulation and wound healing rate in male Wistar rats. Initially, impregnated sterile gauze was prepared from the mixture of bentonite and halloysite minerals and petroleum jelly (Vaseline). Then, the effect of gauze was studied on the blood coagulation time and wound healing process in 40 Wistar rats. SPSS software was used for data analysis and P values less than 0.05 were considered significant. The coagulation time of 81.10 ± 2.532 s in the control group and 33.00 ± 1.214 s in the study group (bentonite-halloysite treated) were reported (P < 0.0005). Time for complete wound healing in the group, which is treated with impregnated sterile pads, was calculated approximately from 10 to 12 days. However, in the control group, there was no complete wound healing (P < 0.0005). According to the results of the present study, topical application of the bentonite-halloysite impregnated sterile gauze significantly decreases the clotting time and increase the wound healing rate.

Topics: Administration, Cutaneous; Aluminum Silicates; Animals; Bandages; Bentonite; Blood Coagulation; Clay; Hemorrhage; Male; Petrolatum; Rats; Rats, Wistar; Whole Blood Coagulation Time; Wound Healing; Wounds, Nonpenetrating