citrinin and Nephrosis

The extent and type of renal ultrastructural changes in Beagle dogs varied with the administration of ochratoxin A and citrinin alone and in the two dosage combinations. The three predominant changes were cytoplasmic vacuolation, myelin figure formation and lesions designated as cytoplasmic disarray. These changes were mainly of the endomembane system of the tubular epithelial cells. Cytoplasmic vacuoles were within proximal and distal tubules and collecting ducts and were most numerous in dogs given 10 mg/kg critrinin. Vacuolation of similar distribution, but less severe, was seen in renal tubular cells of dogs given the higher dose of the combined mycotoxins (0.2 mg/kg ochratoxin A + 10 mg/kg citrinin). This damage was limited to the proximal tubular cells in dogs given only ochratoxin A (0.1 or 0.2 mg/kg). Myelin figures were in proximal epithelial cells of dogs given ochratoxin A alone or combined with citrinin. There was cytoplasmic disarray in dogs of all groups except for dogs given 5 mg/kg citrinin. This lesions was usually limited to the proximal tubules. The lesions, however, was found in cells of the distal tubules of dogs given 10 mg/kg citrinin alone.

Topics: Animals; Benzopyrans; Citrinin; Dog Diseases; Dogs; Kidney Tubules; Kidney Tubules, Distal; Kidney Tubules, Proximal; Male; Nephrosis; Ochratoxins; Vacuoles

Ochratoxin A and citrinin, both mycotoxins, were given separately and combined to young Beagle dogs for 14 days. Ochratoxin A, 0.1 and 0.2 mg/kg, was given by capsule, and citrinin, 5 and 10 mg/kg, was dissolved in ethanol and given by intraperitoneal injection. Clinical signs of toxicosis in dogs given 10 mg/kg citrinin and the higher combined doses included anorexia, retching, tenesmus, weight loss, prostration and death. Severity of the clinical disease and mortality were increased when the mycotoxins were combined, which indicated synergism. The clinicopathological abnormalities reflected renal damage, in that glutamic oxaloacetic transaminase and lactic dehydrogenase increased in the urine of the dogs with clinical signs of poisoning. Serum lactic dehydrogenase was increased in dogs given 10 mg/kg citrinin. Cellular and granular casts, ketones, protein and glucose were in the urine of dogs given large doses of citrinin alone or combined with ochratoxin A. Serum concentrations of sodium, potassium and chloride in the dogs given high doses of each group.

Topics: Animals; Anorexia; Aspartate Aminotransferases; Benzopyrans; Chlorides; Citrinin; Dehydration; Dog Diseases; Dogs; Glycosuria; L-Lactate Dehydrogenase; Male; Nephrosis; Ochratoxins; Potassium; Proteinuria; Sodium

Beagle dogs were given ochratoxin A (0.1 and 0.2 mg/kg) and citrinin (5 and 10 mg/kg) alone and in two dose combinations for 14 days. The gross lesions included focal peritonitis and intestinal intussusceptions in dogs given citrinin. Changes in the kidneys of dogs given ochratoxin A were degeneration and necrosis with desquamation of tubular epithelial cells, primarily in the straight segment of the proximal tubules. Dogs given 10 mg/kg citrin had similar changes in the distal tubules and collecting ducts. Dogs given combined doses of citrinin and ochratoxin A had degeneration and necrosis in proximal and distal tubules, and in thin segments and the collecting ducts; there were desquamated cells and granular casts in the lumina. Dogs given ochratoxin A had necrosis of lymphoid tissues in the spleen, tonsil, thymus, peripheral lymph nodes and lymph nodules of the ileum, colon and rectum. There was ulceration of the mucosa of the intestine in dogs given large combined doses of ochratoxin A and citrinin.

Topics: Animals; Benzopyrans; Citrinin; Dog Diseases; Dogs; Ileum; Intussusception; Kidney; Lymph Nodes; Male; Nephrosis; Ochratoxins