citric-acid--iron--sorbitol-drug-combination and Escherichia-coli-Infections

The contribution of iron towards the free radical generation leading to renal tissue damage was assessed using a non-obstructive ascending mouse model for chronic pyelonephritis. The parameters studied include luminol dependent chemiluminescence (LDCL), histopathology and some biochemical investigations. We found that iron enhanced the renal tissue damage and led to renal scarring, and end point in chronic renal inflammation, irrespective of the bacterial strain studied. In addition a role of iron chelation therapy as a treatment for chronic renal inflammation is also suggested.

Topics: Animals; Chelation Therapy; Chronic Disease; Citric Acid; Deferoxamine; Disease Models, Animal; Drug Combinations; Escherichia coli; Escherichia coli Infections; Female; Ferric Compounds; Free Radicals; Glucosephosphate Dehydrogenase; Glutathione Reductase; Histocytochemistry; Iron; Kidney; L-Lactate Dehydrogenase; Lipid Peroxides; Luminescent Measurements; Mice; Mice, Inbred BALB C; Pyelonephritis; Reactive Oxygen Species; Sorbitol

Administration of iron to rats exacerbated early inflammatory changes of pyelonephritis produced by intravenous inoculation of Escherichia coli. This effect was noted with four of eight strains of E. coli tested and was dependent on bacterial inoculum. Despite this increase in severity of acute pyelonephritis as judged by numbers of bacteria in the kidney and careful gross and microscopic evaluation, there was no enhancement of chronic changes seen 6 months later.

Topics: Abscess; Animals; Cicatrix; Citrates; Citric Acid; Drug Combinations; Escherichia coli Infections; Female; Ferric Compounds; Iron; Kidney; Organ Size; Pyelonephritis; Rats; Rats, Inbred Strains; Sorbitol