chrysin and Pancreatitis

This study was performed to evaluate the protective effects of chrysin (CH) on metabolic impairment and pancreatic injury caused by sub-chronic chlorpyrifos (CPF) intoxication in male rats.. Forty male Wistar rats were randomly allocated into five groups (n=8). Intraperitoneal injections of chrysin (12.5, 25 and 50 mg/kg for 45 days) and CPF (10 mg/kg for 45 days) gavage were performed. Present findings indicated that the serum levels of glucose, total cholesterol, and lowdensity lipoprotein-cholesterol, as well as body weight, were increased in the CPF-exposed group.. It was also found that CPF decreased superoxide dismutase activity as well as increased malondialdehyde and nitric oxide levels in the pancreatic tissue of exposed animals. Histopathological examination also confirmed the toxic effects of CPF on pancreatic tissue as mostly evidenced by infiltration of inflammatory cells and necrosis. CH (50 mg/kg) decreased blood glucose concentration (p < 0.05), TG (p < 0.05), and LDL-C in CPF-exposed animals. CH decreased the pancreas levels of MDA in all treated CPF-exposed groups versus the non-treated CPF-exposed group (p < 0.05, p < 0.001, p < 0.001, respectively). A significant difference was not seen in the NO and MDA levels and SOD activity between CH-treated (50 mg/kg) animals exposed to CPF and controls. A significant difference was not seen in the NO and MDA levels and SOD activity between CHtreated (50 mg/kg) animals exposed to CPF and controls.. A significant difference was not seen in the NO and MDA levels and SOD activity between CH-treated (50 mg/kg) animals exposed to CPF and controls. In conclusion, CH could prevent initiate and progress of CPF-induced metabolic impairment by modulating oxidative stress in pancreatic tissue as a target organ of organophosphorus pesticides.

Topics: Animals; Chlorpyrifos; Cholesterol; Male; Organophosphorus Compounds; Pancreatitis; Pesticides; Rats; Rats, Wistar; Superoxide Dismutase

This experimental study was conducted to evaluate the possible effects of orally administered chrysin on acute pancreatitis.. Twenty four rats were procured. The animals were randomly divided into four groups. In Group I, only vehicle solution (5% dimethylsulfoksid) was administered, and in Group II, chrysin dissolved in the vehicle solution was administered for six days. In Group III and Group IV cerulein was administered to induce acute pancreatitis. In Group III, only vehicle solution was administered, and in Group IV, chrysin dissolved in the vehicle solution was administered orally for six days. Blood samples were analyzed and the pancreatic tissue specimens were evaluated for histopathological examination.. Group III and Group IV, exhibited markedly higher levels of serum WBC, amylase, and lipase, compared with Groups I and II. In the pancreatitis induced groups, CRP and TOS values were found to be significantly higher. In Group II and Group IV, TAS values were significantly higher. The highest calculated OSI values were observed in Group III. Group IV OSI values were significantly lower than those in Group III and even in Group I. Noticeable histopathological changes were identified in the pancreatitis induced Groups III and IV. Compared with Group III, the extent and severity of pancreatic injuries were markedly lower in Group IV.. Chrysin application reduced oxidative stress and histopathological parameters. The present study shows that chrysin can be used to treat pancreatic diseases.. Acute pancreatitis, Cerulein, Chrysin.. Si tratta di uno studio sperimentale finalizzato alla valutazione dei possibili effetti della somministrazione orale di Chrysina sulla pancreatite acuta. Sono stati utilizzati 24 ratti divisi a random in quattro gruppi. Nel primo gruppo è stata somministrata agli animali soltanto la soluzione eccipiente (5% dimethylsulfoksid - DMSO). Nel Gruppo II è stata somministrata per 6 giorni la Chrysina disciolta nella stessa soluzione veicolo. Nel Gruppo III e nel Gruppo IV è stata somministrata Ceruleina per indurre una pancreatite acuta, Nel III Gruppo è stata somministrata la sola soluzione veicolo, mentre nel IV Gruppo è stata somministrata per 6 giorni la Chrysina disciolta nella soluzione veicolante. Sono stati analizzati campioni di sangue e sono stati prelevati campioni di tessuto pancreatico per esame istopatologico. Nei Gruppi III e IV si sono riscontrati più elevati livelli di leucocitosi, di amilasi e lipasi in paragone con i Gruppi I e II. Nei gruppi in cui è stata indotta la pancreatite acuta sono stati riscontrati valori significativamente più alti di Proteina C Reattiva (CRP) e un più elevato stato totale di ossidanti (TOS). Nei Gruppi II e IV i valori di stato totale di antiossidanti (TAS) sono stati rilevati significativamente più elevati. I più alti valori calcolati di Stress ossidative Index (OSI) sono stati riscontrati nel Gruppo III. Nel IV Gi valori di OSI sono risultati significativamente inferiori rispetto a quelli dei Gruppi III ed anche I. Notevoli alterazioni istopatologiche sono state osservate nei gruppi III e IV di pancreatite acuta indotta. A confronto con il Gruppo III la diffusione e la gravità dei danni pancreatici sono stati rilevati significativamente minori nel Gruppo IV. L’utilizzo della Chrysina riduca dunque lo stress ossidativo e i parametri istopatologici, e dunque questo studio indica che la Chrysina può essere usata per il trattamento delle patologie del pancreas.

Topics: Acute Disease; Animals; Disease Models, Animal; Flavonoids; Pancreas; Pancreatitis; Random Allocation; Rats; Rats, Wistar

Chaiqin chengqi decoction (CQCQD) is a Chinese herbal formula derived from dachengqi decoction. CQCQD has been used for the management of acute pancreatitis (AP) in the West China Hospital for more than 30 years. Although CQCQD has a well-established clinical efficacy, little is known about its bioactive ingredients, how they interact with different therapeutic targets and the pathways to produce anti-inflammatory effects.. Toll-like receptor 4 (TLR4) and the nucleotide-binding oligomerization domain-like receptor family pyrin domain containing 3 (NLRP3) inflammasome-mediated pro-inflammatory signaling pathways, play a central role in AP in determining the extent of pancreatic injury and systemic inflammation. In this study, we screened the bioactive ingredients using a pharmacological sub-network analysis based on the TLR4/NLRP3 signaling pathways followed by experimental validation.. The main CQCQD bioactive compounds were identified by UPLC-QTOF/MS. The TLR4/NLRP3 targets in AP for CQCQD active ingredients were confirmed through a pharmacological sub-network analysis. Mice received 7 intraperitoneal injections of cerulein (50 μg/kg; hourly) to induce AP (CER-AP), while oral gavage of CQCQD (5, 10, 15 and 20 g/kg; 3 doses, 2 hourly) was commenced at the 3rd injection of cerulein. Histopathology and biochemical indices were used for assessing AP severity, while polymerase chain reaction, Western blot and immunohistochemistry analyses were used to study the mechanisms. Identified active CQCQD compounds were further validated in freshly isolated mouse pancreatic acinar cells and cultured RAW264.7 macrophages.. The main compounds from CQCQD belonged to flavonoids, iridoids, phenols, lignans, anthraquinones and corresponding glycosides. The sub-network analysis revealed that emodin, rhein, baicalin and chrysin were the compounds most relevant for directly regulating the TLR4/NLRP3-related proteins TLR4, RelA, NF-κB and TNF-α. In vivo, CQCQD attenuated the pancreatic injury and systemic inflammation of CER-AP and was associated with reduced expression of TLR4/NLRP3-related mRNAs and proteins. Emodin, rhein, baicalin and chrysin significantly diminished pancreatic acinar cell necrosis with varied effects on suppressing the expression of TLR4/NLRP3-related mRNAs. Emodin, rhein and chrysin also decreased nitric oxide production in macrophages and their combination had synergistic effects on alleviating cell death as well as expression of TLR4/NLRP3-related proteins.. CQCQD attenuated the severity of AP at least in part by inhibiting the TLR4/NLRP3 pro-inflammatory pathways. Its active ingredients, emodin, baicalin, rhein and chrysin contributed to these beneficial effects.

Topics: Acinar Cells; Animals; Anti-Inflammatory Agents, Non-Steroidal; Ceruletide; Drugs, Chinese Herbal; Emodin; Flavonoids; Inflammasomes; Male; Mice; Mice, Inbred C57BL; NLR Family, Pyrin Domain-Containing 3 Protein; Pancreatitis; RAW 264.7 Cells; Toll-Like Receptor 3