chrysin and Mouth-Neoplasms

Chrysin is a flavonoid found abundantly in substances, such as honey and phytochemicals, and is known to exhibit anticancer effects against various cancer cells. Nevertheless, the anticancer effect of chrysin against oral cancer has not yet been verified. Furthermore, the mechanism underlying autophagy is yet to be clearly elucidated. Thus, this study investigated chrysin-mediated apoptosis and autophagy in human mucoepidermoid carcinoma (MC-3) cells. The change in MC-3 cell viability was examined using a 3-(4,5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide cell viability assay, as well as 40,6-diamidino-2-phenylindole, annexin V, and propidium iodide staining. Western blotting was used to analyze the proteins related to apoptosis and the mitogen-activated protein kinase (MAPK) pathway. In addition, the presence or absence of autophagy and changes in the expression of related proteins were investigated using acridine orange staining and Western blot. The results suggested that chrysin induced apoptosis and autophagy in MC-3 oral cancer cells via the MAPK/extracellular signal-regulated kinase pathway. Moreover, the induced autophagy exerted a cytoprotective effect against apoptosis. Thus, the further reduced cell viability due to autophagy as well as apoptosis induction highlight therapeutic potential of chrysin for oral cancer.

Topics: Apoptosis; Autophagy; Cell Line, Tumor; Extracellular Signal-Regulated MAP Kinases; Flavonoids; Humans; Mouth Neoplasms; TOR Serine-Threonine Kinases

To investigate the effect of chrysin on apoptosis of oral squamous carcinoma KB cell line and the possible mechanisms, and to provide new ideas for the treatment of oral cancer.
 Methods: Oral cancer KB cells were treated with different concentrations of chrysin (1, 2, 4, 8, 16, and 32 μmol/L) for 24 h. Cell proliferation was detected by MMT assay; apoptosis was detected by flow cytometry; the activity of caspase-3/7 was detected by chemiluminescent assay; mitochondrial membrane potential in KB cells was determined by JC-1 assay; and Western blotting was used to determine the activation of protein kinase B (AKT) and phosphoinositide-3-kinase (PI3K).
 Results: Chrysin inhibited the proliferation of KB cells in a concentration-dependent manner, accompanied by increase in apoptosis of KB cells, activation of caspase-3/7, decrease in mitochondrial membrane potential, and suppression of the phosphorylation of AKT and PI3K.
 Conclusion: The effect of chrysin on KB cell apoptosis may be related to mitochondrial dysfunction and inhibition of PI3K/AKT pathway.. 目的：探讨白杨素对口腔鳞状细胞癌KB细胞凋亡的影响及其机制，为临床上口腔鳞状细胞癌的治疗提供思路。方法：用不同浓度白杨素(1，2，4，8，16和32 μmol/L)处理KB细胞24 h，采用MMT法检测细胞增殖，流式细胞术检测细胞凋亡，化学发光法检测caspase-3/7活性，JC-1法检测KB细胞线粒体膜电位的变化，蛋白质印迹检测蛋白激酶B(protein kinase B，AKT)和磷脂酰肌醇3-激酶(phosphoinositide-3-kinase，PI3K)的活化。结果：白杨素以浓度依赖方式抑制KB细胞增殖并诱导其凋亡，促进caspase-3/7的活化，降低KB细胞线粒体膜电位；同时抑制AKT和PI3K磷酸化。结论：白杨素诱导KB细胞凋亡作用可能与线粒体功能障碍和抑制PI3K/AKT通路相关。.

Topics: Apoptosis; Carcinoma, Squamous Cell; Flavonoids; Humans; KB Cells; Mouth Neoplasms; Phosphatidylinositol 3-Kinases; Signal Transduction

Combinations of natural bee wax flavones chrysin with a chemo drug have been exhibiting high potential with reduced adverse effect. To extend the synergistic effect of chrysin and improve the MLNPs (Multi Layer Nanoparticles) performance in drug release, layer-by-layer of poly [di(sodium carboxyphenoxy)phosphazene] (PDCPP) and poly (diallyldimethyl ammonium chloride) (PDADMAC) deposited on the CaCO

Topics: Animals; Antineoplastic Agents; Apoptosis; Carcinoma; Cell Line, Tumor; Cisplatin; Delayed-Action Preparations; Drug Carriers; Drug Delivery Systems; Drug Liberation; Drug Synergism; Flavonoids; Humans; KB Cells; Male; Mesocricetus; Mitochondrial Diseases; Mouth Neoplasms; Nanoparticles; Polyethylenes; Quaternary Ammonium Compounds; Reactive Oxygen Species