chromomycins and Fibrosarcoma

chromomycins has been researched along with Fibrosarcoma* in 2 studies

Other Studies

2 other study(ies) available for chromomycins and Fibrosarcoma

ArticleYear
Mode of action of antitumour antibiotics. spectrophotometric studies on the interaction of chromomycin A3 with DNA and chromatin of normal and neoplastic tissue.
    Biochimica et biophysica acta, 1975, Jan-20, Volume: 378, Issue:2

    The binding of chromomycin A3, an antitumour antibiotic, to various DNA and chromatin isolated from mouse and rat liver, mouse fibrosarcoma and Yoshida ascites sarcoma cells was studied spectrophotometrically at 29 degrees C in 10-2 M Tris-HCl buffer, pH 8.0, containing small amounts of MgCl2 (4.5-10-5--25-10-5 M). An isobestic point at 415 nm was observed when chromomycin A3 was gradually titrated with DNA/chromatin and its spectrum shifted towards higher wavelength. The rates and extent of these spectral changes were found to be dependent on the concentration of Mg2+. The change in absorbance at 440 nm was used to calculate apparent binding constant (Kap M-1) and sites per nucleotide (n) from Scatchard plots for various DNA and chromatins. As expected, values of n for chromatin (0.06-0.10) were found to be lower than found for corresponding DNA (0.10-0.15). Apparently no such correlation exists between binding constants (Kap M-1)-10-4) of DNA (6.4--11.2) and of chromatin (3.1--8.3), but Kap M-1 of chromatin isolated from mouse fibrosarcoma and Yoshida ascites sarcoma are 1.5--3 times higher than that found for mouse and rat liver chromatin. These differences may be taken to indicate structural difference in nucleoprotein complexes caused by neoplasia. The relevance of this finding to tumour suppressive action of chromomycin A3 is discussed.

    Topics: Animals; Binding Sites; Chromatin; Chromomycins; DNA; DNA, Neoplasm; Fibrosarcoma; Kinetics; Liver; Magnesium; Mice; Rats; Sarcoma, Yoshida; Spectrophotometry

1975
Studies on the effect of some antitumour antibiotics on mouse fibrosarcoma.
    The Indian journal of medical research, 1975, Volume: 63, Issue:7

    Topics: Animals; Chromomycins; Dactinomycin; Dose-Response Relationship, Drug; Female; Fibrosarcoma; Male; Mice; Sarcoma, Experimental

1975