chondroitin-sulfates and Histiocytoma--Benign-Fibrous

Viscous material secreted from NMSG 10 cells cultured from malignant fibrous histiocytoma of human bone was demonstrated using two-dimensional electrophoresis to be glycosaminoglycans (GAGs) consisting of mainly hyaluronic acid (HA), along with small amounts of heparan sulfate (HS) and chondroitin sulfate (ChS). The biosynthesis of HA in a cell-free system was located at the plasma membrane fraction by colloidal-iron-reactive ultrastructural staining. The molecular weight of the HA synthesized by the plasma membrane fraction was estimated to be about 90,000 D by gel filtration. The material secreted by the original tumor tissue of NMSG 10 cells was demonstrated to be GAGs consisting of HA, HS, ChS and dermatan sulfate using two-dimensional electrophoresis. These findings suggest that transformed tumor cells could synthesize HA at the plasma membrane and release it into the stroma of tumor tissue.

Topics: Alcian Blue; Bone Neoplasms; Cell Membrane; Cell-Free System; Chondroitin Sulfates; Dermatan Sulfate; Heparitin Sulfate; Histiocytoma, Benign Fibrous; Humans; Hyaluronic Acid; Hyaluronoglucosaminidase; Staining and Labeling; Tumor Cells, Cultured