chondroitin-sulfates and Dentigerous-Cyst

Histochemical examination of odontogenic cyst connective tissue has demonstrated that hyaluronic acid is the predominant glycosaminoglycan present with lesser amounts of the sulphated glycosaminoglycans. Mast cells were frequently observed in the connective tissue, particularly just beneath the epithelium and may be responsible for the enhanced staining for heparin observed in this area. Local tissue metabolism and inflammation will contribute to the release of glycosaminoglycans from connective tissue into the lumenal fluid. Staining of glycosaminoglycans was generally minimal in the epithelium, although hyaluronic acid and chondroitin sulphate were detected in goblet cells. Glycosaminoglycans previously demonstrated in cyst fluids are probably derived from several sources in the cyst and will be important in its expansile growth.

Topics: Chondroitin Sulfates; Dentigerous Cyst; Glycosaminoglycans; Histocytochemistry; Humans; Hyaluronic Acid; Hydrogen-Ion Concentration; Odontogenic Cysts; Radicular Cyst; Staining and Labeling

Glycosaminoglycans (GAG) were extracted from the connective tissue of 15 dental cysts, 2 dentigerous cysts and 7 keratocysts, and separated electrophoretically and chromatographically. Hyaluronic acid (dental, 2.38; dentigerous, 3.98; keratocyst, 3.19 micrograms uronic acid/mg lyophilized tissue) was the major GAG detected, with lesser amounts of heparin (dental, 1.70; dentigerous, 1.95; keratocyst, 1.80 micrograms uronic acid/mg lyophilized tissue) and chrondroitin-4-sulphate (dental, 1.33; dentigerous, 2.85; keratocyst, 1.37 micrograms uronic acid/mg lyophilized tissue). It is suggested that enzymic release of these GAGs allows their diffusion into the luminal fluid where they may contribute to expansile cyst growth.

Topics: Chondroitin Sulfates; Connective Tissue; Dentigerous Cyst; Glycosaminoglycans; Heparin; Humans; Hyaluronic Acid; Odontogenic Cysts; Radicular Cyst

Glycosaminoglycans and proteoglycans were analysed in keratinizing and nonkeratinizing odontogenic cyst fluids. Hyaluronic acid showed the highest incidence and abundance amongst the glycosaminoglycans detected. Appreciable amounts of chondroitin-4-sulphate were also observed, particularly in the dental cysts, with lesser amounts of the other glycosaminoglycans. Heparan sulphate showed a higher incidence and abundance in the keratocyst than the other cysts, whilst chondroitin-6-sulphate could not be detected in any of the cysts. A considerable proportion of the glycosaminoglycans of the fluids appeared to be complexed with protein and was released only after proteolytic digestion. The origin of these macromolecules is uncertain although it is likely that they are derived from both the connective tissue and the epithelium of the cyst wall.

Topics: Ameloblastoma; Chondroitin Sulfates; Dentigerous Cyst; Dermatan Sulfate; Electrophoresis, Cellulose Acetate; Glycosaminoglycans; Heparitin Sulfate; Hexuronic Acids; Humans; Hyaluronic Acid; Jaw Neoplasms; Odontogenic Cysts