chondroitin-sulfates and Circoviridae-Infections

Porcine circovirus type 2 (PCV2) is the etiological agent of PCV2-associated diseases (PCVAD). PCV2 targets lymphoblasts, and pigs suffering from PCVAD display lymphocyte depletion in lymphoid tissues. PCV2 infection of lymphoblasts has not been studied. Here, the replication cycle of PCV2 (abortion strain 1121 and PMWS strain Stoon1010) in T-lymphoblasts was examined. The expression of Rep and Cap were found for both viral strains, while progeny virus was detected for Stoon1010 but not for 1121. PCV2 attached to 11-26% (1121-Stoon1010) of the T-lymphoblasts while 2.6-12.7% of cells showed virus internalization. Chondroitin sulfate (CS) was present on 25% of T-lymphoblasts, and colocalized with PCV2 on 31-32% of the PCV2+ cells. Enzymatic removal of CS reduced PCV2 infection. PCV2 infection was decreased by chlorpromazine, cytochalasin D and

Topics: Actins; Animals; Capsid Proteins; Cells, Cultured; Chondroitin Sulfates; Circoviridae Infections; Circovirus; Clathrin; Endocytosis; Endosomes; Host-Pathogen Interactions; Hydrogen-Ion Concentration; Models, Molecular; Monomeric GTP-Binding Proteins; Nucleic Acids; Serine Proteases; Species Specificity; Swine; T-Lymphocytes; Virus Attachment; Virus Internalization; Virus Replication; Virus Uncoating