chondroitin and Glioma

Topics: Cell Cycle Proteins; Cell Line, Tumor; Cell Movement; Cell Proliferation; Chondroitin; Chromatography, Liquid; Gene Expression Regulation, Neoplastic; Glioma; Hepatocyte Nuclear Factor 4; Hepatocyte Nuclear Factors; Humans; Tandem Mass Spectrometry

A polyclonal rabbit antiserum was utilized to localize chondroitin sulfate in human gliomas. Tissue sections were digested with chondroitinase ABC to create the antigenic determinant on the chondroitin sulfate proteoglycan molecule. Normal CNS tissue showed a positive immunohistochemical staining both in white and gray matter, sparing the cytoplasm of glial and neuronal cells. Differentiated astrocytomas presented the same pattern as the normal CNS. Anaplastic astrocytomas and glioblastomas showed progressive reduction of parenchymal positivity as anaplasia increased. These data suggest that chondroitin sulfate is a character expressed by differentiated CNS cells and that it is lost with dedifferentiation. Vascular structures presented positive material in the adventitia in all the oncotypes. A discontinuous positivity was observed in the basal membrane zone of the vessels.

Topics: Astrocytoma; Brain Neoplasms; Chondroitin; Chondroitin Sulfates; Glioma; Histocytochemistry; Humans; Immunologic Techniques

Topics: Brain; Brain Neoplasms; Cell Transformation, Neoplastic; Chondroitin; Chondroitin Sulfates; Glioma; Humans

Previous studies have shown that D-xylose partially overcomes the puromycin inhibition of chondroitin sulfate synthesis in cultured chick embryo chondrocytes. Likewise, D-xylose stimulates chondroitin sulfate synthesis by limb bud mesenchyme cells previously treated with BrdU or limb bud cartilage cells treated with puromycin. The studies reported here show that p-nitrophenyl-beta-D-xylopyranoside and 4-methyl-umbelliferyl-beta-D-xylopyranoside cause a much greater stimulation than does D-xylose and are active at much lower concentrations. In contrast to D-xylose, the xylosides strikingly stimulate chondroitin sulfate synthesis in predifferentiated mesenchyme cells. The xylosides stimulate synthesis of chondroitin sulfate by rat glial cell tumor cells (RC-6), a mouse neuroblastoma (C1300, NB41A), and two strains of cultured rat hepatoma cells (HTC, H(4)). These results indicate that certain types of nonconnective tissue cells contain the enzymic machinery for synthesis of chondroitin sulfate which is normally not utilized because of limited synthesis of core protein and/or xylosyltransferase. The beta-xylosides may be used as a probe of the capacity of various cell types to synthesize sulfated glycosaminoglycans.

Topics: Animals; Bromodeoxyuridine; Carcinoma, Hepatocellular; Cartilage; Cell Line; Cells, Cultured; Chick Embryo; Chondroitin; Coumarins; Glioma; Glycosaminoglycans; Glycosides; Liver Neoplasms; Mice; Neuroblastoma; Puromycin; Rats; Stimulation, Chemical; Xylose

A clonal strain of rat glial tumor cells was shown to produce the acid mucopolysaccharides, hyaluronic acid, chondroitin 4-SO(4), and heparan sulfate in tissue culture. The mucopolysaccharides were isolated both from the growth medium and the cells.

Topics: Animals; Cattle; Chondroitin; Culture Techniques; Glioma; Glycosaminoglycans; Hyaluronic Acid; Rats; Sulfur Isotopes; Tritium