chondroitin and Cell-Transformation--Neoplastic

Topics: Cell Transformation, Neoplastic; Cells, Cultured; Chemical Phenomena; Chemistry; Chondroitin; Chondroitin Sulfates; Chromatography, Gel; Dermatan Sulfate; Fibroblasts; Glycosaminoglycans; Heparin; Humans

Topics: Cell Line; Cell Transformation, Neoplastic; Chondroitin; Chondroitin Sulfates; Disaccharides; Glycosaminoglycans; Heparitin Sulfate

Topics: Brain; Brain Neoplasms; Cell Transformation, Neoplastic; Chondroitin; Chondroitin Sulfates; Glioma; Humans

The relative amount of sulfated glycosaminoglycans associated with the cell layer of parent and SV40-transformed Swiss mouse 3T3 cells was determined from the incorporation of labeled sulfate (35SO4) into macromolecular material. In cultures of SV40-transformed cells, the glycosaminoglycan content per cell was constant over a wide range of densities. In cultures of parent 3T3 cells, the glycosaminoglycan content per cell increased directly with density, the highest values being found in contact-inhibited cultures. At high cell densities, the glycosaminoglycan content of 3T3 cells was several-fold higher than that for SV40-transformed cells. Most of the density-dependent increase in glycosaminoglycans of 3T3 cells was accounted for by chondroitin sulfate (dermatan sulfate) which was over 6-fold higher in confluent cultures than in low density cultures.

Topics: Cell Count; Cell Line; Cell Transformation, Neoplastic; Chondroitin; Chondroitin Sulfates; Glycosaminoglycans; Heparitin Sulfate

Balb/c 3T3 cells synthesize 5--10 times more 35SO2/4- -labeled extracellular proteoglycan per cell than do Balb/c 3T3 cells transformed by SV40 (SV3T3). The extracellular 35SO2/4- -labeled proteoglycans of the Balb/c 3T3 and SV3T3 cells differ markedly in their acid mucopolysaccharide composition. Extracellular Balb/c 3T3 proteoglycans contain about 70--80% chondroitin sulfate, most of which is chondroitin 4-sulfate, and small amounts of heparan sulfate and/or heparin. On the other hand, extracellular SV3T3 proteoglycans contain 65-75% heparan sulfate and/or heparin and less than 15% chondroitin sulfate. Analysis of extracellular 35SO2/4- -labeled proteoglycan by sodium dodecyl sulfate-polyacrylamide gel electrophoresis reveals that Balb/c 3T3 alone synthesizes a class of proteoglycans capable of migrating in a 10% separating gel. This class of proteoglycans, designated as fraction C, accounts for up to 45% of the total extracellular Balb/c 3T3 35 SO2/4- -labeled proteoglycans and contains chondroitin sulfate extracellular SV3T3 proteoglycans. The absence of this and other classes of chondroitin sulfate-containing proteoglycans can account for the 5-10-fold decreased synthesis of 35SO2/4- -labeled proteoglycans by SV3T3 cells when compared to Balb/c 3T3 cells.

Topics: Cell Line; Cell Transformation, Neoplastic; Chondroitin; Chondroitin Sulfates; Chondroitinases and Chondroitin Lyases; Glycoside Hydrolases; Hyaluronoglucosaminidase; Proteoglycans; Simian virus 40

Topics: Cell Division; Cell Line; Cell Transformation, Neoplastic; Chondroitin; Chondroitin Sulfates; Galactosamine; Glucosamine; Glycopeptides; Glycosaminoglycans; Heparitin Sulfate; Hyaluronic Acid

Topics: Animals; Cell Transformation, Neoplastic; Cells, Cultured; Chondroitin; Chromatography, DEAE-Cellulose; Chromatography, Gel; Glycosaminoglycans; Heparitin Sulfate; Hexosamines; Hyaluronic Acid; Mice; RNA; Simian virus 40; Trypsin; Uridine

The rate of hyaluronic acid and sulfated mucopolysaccharide production was measured for hamster embryo fibroblasts and for general oncogenic lines derived by virus transformation. A striking increase in both the rate of hyaluronic acid synthesis and the amount of cell-associated polymer was observed after transformation by herpes simplex type-2 or SV40 virus. Although no corresponding change was observed for the sulfated polysaccharides, the proportion of heparan sulfate increased significantly after transformation.

Topics: Animals; Cell Line; Cell Transformation, Neoplastic; Cells, Cultured; Chondroitin; Cricetinae; Embryo, Mammalian; Fibroblasts; Glucosamine; Glycosaminoglycans; Haplorhini; Heparin; Heparitin Sulfate; Hexosaminidases; Hyaluronic Acid; Hyaluronoglucosaminidase; Kidney; Kinetics; Simian virus 40; Simplexvirus; Tritium

Coarse powders of acid-insoluble matrix of diaphysis and calvarial parietal bone rapidly and consistently transformed fibroblasts into masses of cartilage and bone containing hemopoietic marrow. The transformant was encapsulated by fibroblasts within 24 hr to form a plaque. Transformation was restricted to the central thicknesses of the plaque. Under the stated conditions the alteration of the phenotype, fibroblast to chondroblast, was an unstable transformation, whereas the phenotype change, fibroblast to osteoblast, was stable. The transformation occurred on a rigid timetable of sequences. Measurements of alkaline phosphatase activity and incorporation of radioactive sulfate, phosphate, and calcium were sensitive and quantitative assays for the appearance of the transformed products, cartilage and bone.

Topics: Alkaline Phosphatase; Animals; Biotransformation; Bone and Bones; Bone Matrix; Calcium; Cartilage; Cell Transformation, Neoplastic; Chondroitin; Electrophoresis, Paper; Female; Fibroblasts; Hematopoiesis; Male; Phenotype; Phosphorus; Rats; Rats, Inbred Strains; Species Specificity; Sulfur; Tissue Survival; Transplantation, Homologous

Topics: Animals; Carcinoma; Carcinoma, Ehrlich Tumor; Cell Line; Cell Transformation, Neoplastic; Chondroitin; Cricetinae; Culture Media; Culture Techniques; Fibroblasts; Glycosaminoglycans; HeLa Cells; Humans; Leukemia L1210; Lyases; Lymphoma; Mice; Mouth Neoplasms; Sarcoma 180; Secretory Rate; Simian virus 40; Sodium; Species Specificity; Sulfates; Sulfur Isotopes; Sulfuric Acids

Topics: Animals; Cell Line; Cell Transformation, Neoplastic; Chondroitin; Clone Cells; Collagen; Cricetinae; Culture Techniques; Depression, Chemical; Dextrans; Heparin; Kidney; Mucoproteins; Polyomavirus; Polysaccharides