chondroitin and Carcinoma--Ehrlich-Tumor

The biosynthesis and intracellular distribution of acid mucopolysaccharides in Ehrlich ascites tumor cells was studied in vivo by means of the precursors 35S-sulfate, 3H-glucosamine and 14C-galactosamine. It was found that the acid mucopolysaccharide present in the ascitic fluid supernatant is hyaluronic acid. Hyaluronic acid appears to be of extracellular origin, and it is bound to proteins of the cell membrane. The ascites cells exhibit a very active production of sulfated mucopolysaccharides particularly at the mitochondria and cell membrane level. Chondroitin sulfate A is the major component but also the isomers B and C are present. The possible role of chondroitin sulfate A in the development of neoplastic characteristics of the cell is discussed.

Topics: Animals; Ascitic Fluid; Carcinoma, Ehrlich Tumor; Chondroitin; Glycosaminoglycans; Hyaluronic Acid; Mice; Subcellular Fractions

Topics: Animals; Carcinoma, Ehrlich Tumor; Cells, Cultured; Chondroitin; Glycosaminoglycans; Heparin; Hyaluronic Acid; In Vitro Techniques; Leukemia, Experimental; Lymphoma, Non-Hodgkin; Mammary Neoplasms, Experimental; Mice; Neoplasms, Experimental; Sarcoma 180; Sarcoma, Experimental

Topics: Animals; Antineoplastic Agents; Basidiomycota; Carcinoma, Ehrlich Tumor; Chondroitin; Female; Male; Mammary Neoplasms, Experimental; Mice; Neoplasms, Experimental; Plant Extracts; Poly I-C; Sarcoma 180

The effect of various glycosidases on the growth of solid hypotetraploid Ehrlich ascites tumour was investigated. The purified bacterial chondroitinase-ABC significantly inhibited the growth of tumour; chondroitinase-AC inhibited the tumour to some extent but chondro-4-sulphatase, chondro-6-sulphatase, streptomyces hyaluronidase, and β-glucuronidase had no inhibitory effect. Heat-treated chondroitinase-ABC had no inhibitory effect on the tumour growth. The growth of tumour cells which were injected subcutaneously after in vitro incubation with chondroitinase-ABC or -AC solution was decreased when compared with that of sham-treated cells.The injection of 1 ml of chondroitin sulphate A and chondroitin sulphate C solution prior to tumour inoculation into the same site promoted the tumour growth, while growth-stimulating effect of chondroitin sulphate B was ambiguous. The chondroitin sulphate appears to serve as a growth supporter which protects the surface of tumour cells and promotes the physiological surface function of the cells.

Topics: Animals; Carcinoma, Ehrlich Tumor; Chondroitin; Glucuronidase; Hyaluronoglucosaminidase; Lyases; Male; Mice; Peritoneal Neoplasms; Sulfates

Topics: Animals; Carcinoma, Ehrlich Tumor; Chondroitin; Collagen; Connective Tissue; Glycosaminoglycans; Hexosamines; Hydrocortisone; Hydroxyproline; Injections, Intraperitoneal; Injections, Subcutaneous; Neoplasm Transplantation; Ovalbumin; Uronic Acids

Topics: Animals; Carcinoma; Carcinoma, Ehrlich Tumor; Cell Line; Cell Transformation, Neoplastic; Chondroitin; Cricetinae; Culture Media; Culture Techniques; Fibroblasts; Glycosaminoglycans; HeLa Cells; Humans; Leukemia L1210; Lyases; Lymphoma; Mice; Mouth Neoplasms; Sarcoma 180; Secretory Rate; Simian virus 40; Sodium; Species Specificity; Sulfates; Sulfur Isotopes; Sulfuric Acids

Topics: Animals; Carcinoma, Ehrlich Tumor; Chondroitin; In Vitro Techniques; Injections, Subcutaneous; Male; Mice; Neoplasm Transplantation; Statistics as Topic; Sulfates

Topics: Animals; Blood Platelets; Carcinoma, Ehrlich Tumor; Cell Membrane; Chondroitin; Cytosine Nucleotides; Electric Conductivity; Electrophoresis; Glycosaminoglycans; Humans; Interferons; Mice; Neuraminic Acids; Polynucleotides; Stimulation, Chemical; Surface Properties

Topics: Animals; Bromides; Carcinoma, Ehrlich Tumor; Chondroitin; Colloids; Cytochromes; Heparin; Histones; In Vitro Techniques; Iron; Macrophages; Mice; Microscopy, Electron; Phagocytosis; Protamines; Serum Albumin, Bovine; Sodium; Sulfates

Topics: Animals; Carcinoma, Ehrlich Tumor; Chondroitin; Collagen; Dentistry; Fibrinogen; Hydroxyproline; Neoplasms, Experimental

Topics: Animals; Carcinoma, Ehrlich Tumor; Chondroitin; Muramidase; Neoplasms, Experimental; Stimulation, Chemical

Topics: Animals; Carcinoma, Ehrlich Tumor; Chondroitin; Collagen; Fibrinogen; Hydroxyproline; Male; Mice; Muramidase; Ovalbumin

Topics: Animals; Carcinoma, Ehrlich Tumor; Chondroitin; Injections, Intraperitoneal; Injections, Subcutaneous; Male; Mice; Neoplasm Transplantation

Topics: Animals; Carcinoma, Ehrlich Tumor; Carcinoma, Hepatocellular; Chondroitin; In Vitro Techniques; Liver Neoplasms; Mice; Neoplasms, Experimental; Rats

Topics: Animals; Carcinoma, Ehrlich Tumor; Chondroitin; Galactose; Glucosamine; Glucuronates; Hyaluronoglucosaminidase; Hydrochloric Acid; Male; Mice; Sodium Chloride; Sulfates

Topics: Animals; Carcinoma, Ehrlich Tumor; Chick Embryo; Chondroitin; Collagen; Dextrans; Fibrinogen; Heparin; Hyaluronic Acid; In Vitro Techniques; Methylcellulose; Mice; Povidone

Topics: Animals; Carcinoma, Ehrlich Tumor; Chondroitin; Hydrocortisone; Male; Mice

Topics: Animals; Carcinoma, Ehrlich Tumor; Chick Embryo; Chondroitin; Extraembryonic Membranes; In Vitro Techniques; Male; Mice