cholecystokinin and Cerebral-Infarction

A small surgical lesion of the parietal cortex induces an increase in the expression of several messenger RNAs varying from 172 to 980% in the entire homolateral cerebral cortex, as detected by quantitative in situ hybridization histochemistry. The messenger RNAs encoding the immediate early genes of the leucine zipper family (c-fos, c-jun, jun-B), the Zinc finger family (zif268), the glucocorticoid receptor family (NGFI-B) and the interferon family (PC4) are increased within 2 h after the lesion and return to normal levels at 6 h. The messenger RNAs encoding cholecystokinin, neuropeptide Y, somatostatin and the synthetizing enzyme of the neurotransmitter GABA, glutamate decarboxylase, are elevated within one day and return to normal levels after six days. An intraperitoneal injection of the N-methyl-D-aspartate receptor antagonist dizocilpine maleate, 30 min before surgery, prevented either the induction of immediate early gene expression or the increase of neuropeptide and glutamate decarboxylase messenger RNA expression. This study demonstrates that a minimal cortical lesion induces extensive changes in gene expression and that the mechanism(s) leading to these changes involves the action of glutamate at the N-methyl-D-aspartate receptor. These modifications may be of importance in explaining diffuse changes not related to neuronal circuitry in several conditions.

Topics: Animals; Cerebral Cortex; Cerebral Infarction; Cholecystokinin; Dizocilpine Maleate; DNA-Binding Proteins; Early Growth Response Protein 1; Female; Gene Expression Regulation; Genes, fos; Genes, Immediate-Early; Genes, jun; Glutamate Decarboxylase; Glutamates; Glutamic Acid; Immediate-Early Proteins; Leucine Zippers; Membrane Proteins; N-Methylaspartate; Nerve Tissue Proteins; Neuropeptide Y; Neurotransmitter Agents; Nuclear Receptor Subfamily 4, Group A, Member 1; Parietal Lobe; Rats; Rats, Wistar; Receptors, Cytoplasmic and Nuclear; Receptors, Steroid; Somatostatin; Time Factors; Transcription Factors; Zinc Fingers