cholecalciferol and Postoperative-Cognitive-Complications

Neuroinflammation plays a dominant role in the progression of postoperative cognitive dysfunction (POCD). Vitamin D has been known to have important regulatory functions in inflammation and immune response. The NOD-like receptor protein 3 (NLRP3) is an essential inflammasome in the inflammatory response and could be activated by anesthesia and surgery. In this study, male C57BL/6 mice aged 14-16 months were given VD3 for 14 days straight before having an open tibial fracture surgery. The animals were either sacrificed to obtain the hippocampus or tested in a Morris water maze test. Western blot was employed to estimate the levels of NLRP3, ASC, and caspase-1, immunohistochemistry was used to identify microglial activation, and an enzyme-linked immunosorbent assay was used to measure the expression of IL-18 and IL-1

Topics: Animals; Cholecalciferol; Cognitive Dysfunction; Dietary Supplements; Inflammasomes; Male; Mice; Mice, Inbred C57BL; Neuroinflammatory Diseases; NLR Family, Pyrin Domain-Containing 3 Protein; Postoperative Cognitive Complications