cholecalciferol and Infertility--Female

Assisted reproductive technologies (ART) are the main approach to restore fertility in patients with polycystic ovary syndrome (PCOS). The key milestones to achieve this result include the correct diagnosis, pre-pregnancy pre-treatment, pregnancy planning and the choice of infertility treatment.. The aim of this work was to evaluate the effect of a comprehensive method of pre-pregnancy pre-treatment which includes a vitamin complex with inositol and vitamin D3, on the levels of pituitary and ovarian hormones in women with infertility against the background of polycystic ovary syndrome.. The study involved 111 women with infertility and PCOS, who were offered different methods of pre-pregnancy pre-treatment prior to undergoing controlled ovarian stimulation according to a short protocol with recombinant gonadotropin corifollitropin alfa (Elonva®).. Our results indicate that in women with infertility against the background of PCOS the use of treatment which includes inositol at a dose of 2000 mg/day and vitamin D3 in an average prophylactic dose of 2 drops 2-3 times a day, depending on the concentration of 25(OH)D in blood serum, for 8 weeks, reduces the level of androgens and increases the level of oestrogen, suggesting normalisation of ovulation; while on the day of ovulation trigger the levels of oestradiol and progesterone were significantly lower (1.14 and 1.50 times, respectively) compared to a standard scheme of controlled ovarian stimulation with folic acid in pre-pregnancy pre-treatment.. These results indicate effectiveness of using a combination of a vitamin complex with inositol and vitamin D3 as a pre-pregnancy pre-treatment for the correction of endocrine and metabolic disorders in women with PCOS.

Topics: Cholecalciferol; Female; Humans; Infertility, Female; Inositol; Ovulation Induction; Polycystic Ovary Syndrome; Pregnancy; Vitamins

To compare E-selectin, resistin and reactive oxygen species (ROS) levels in serum and follicular fluid (FF) of subfertile women undergoing Controlled Ovarian Hyperstimulation (COH) during IVF/ICSI cycles, using GnRH-agonist and -antagonist protocols.. In this prospective cohort study, 85 subfertile women undergoing IVF/ICSI were included. Participants underwent the GnRH-agonist and -antagonist protocols; and blood samples were collected at three time points: basic (at start of COH), on the day of hCG and at oocyte retrieval (OR); and from the FF from the first follicle aspirate. Clinical and IVF cycle characteristics, were compared between groups, together with the levels of E-selectin, resistin and ROS in serum and FF, through ELISA. Their prognostic value on pregnancy outcomes was examined.. Examining molecules levels are increasing in serum, from start of COH until OR, irrespectively of the protocol used; FF levels at OR were similar to those in serum at that day. Resistin FF levels were lower in GnRH agonists, compared with the antagonist protocol. Resistin levels at start of COH were associated with clinical pregnancy rates, and this remained significant following adjustment for age, BMI and IVF protocol used, while values of >13.5 ng/ml were associated with a six times greater odd of a pregnancy.. E-selectin, resistin and ROS levels are increasing during COH, reaching their highest values at OR, with comparable values measured in the FF at that time. Resistin values >13.5 ng/ml are linked with a 6-fold increase on the odds of a pregnancy.

Topics: Adult; Ascorbic Acid; Cholecalciferol; Dehydroepiandrosterone; E-Selectin; Female; Fertilization in Vitro; Follicular Fluid; Gonadotropin-Releasing Hormone; Humans; Infertility, Female; Nicotinic Acids; Ovulation Induction; Plant Extracts; Predictive Value of Tests; Pregnancy; Pregnancy Outcome; Prospective Studies; Reactive Oxygen Species; Resistin