chlortetracycline and Hypertension

The effects of erythropoietin (EPO) on cytosolic free calcium concentration ([Ca2+]i) in platelets of 20 essential hypertensive patients (HT) and of 25 normotensive subjects (NT) were investigated using the fura2 technique. In resting platelets [Ca2+]i were not significantly higher in HT compared to NT (74.3 +/- 7.8 nM vs 59.8 +/- 7.0 nM, mean +/- SEM). Addition of EPO significantly increased [Ca2+]i in HT compared to NT (13.8 +/- 5.3 nM vs 0.9 +/- 1.9 nM, p less than 0.01). EPO increased the amount of calcium in intracellular stores. This was confirmed independently using thrombin-induced changes of [Ca2+]i in a calcium-free medium and using chlorotetracycline as a marker of stored calcium. After preincubation with EPO thrombin-induced changes of [Ca2+]i were significantly lower in HT compared to NT (306.1 +/- 30.0 nM vs 407.7 +/- 35.7 nM, p less than 0.05). In a calcium-free medium after preincubation with EPO thrombin-induced changes of [Ca2+]i were significantly lower in HT compared to NT (54.7 +/- 11.8 nM vs 100.9 +/- 10.5 nM, p less than 0.05) indicating lower storage capacity in HT. It is concluded that elevated response to EPO may provide a powerful tool to evaluate diagnosis and underlying pathophysiological mechanisms in essential hypertension.

Topics: Adult; Blood Platelets; Calcium; Chlortetracycline; Erythropoietin; Female; Fura-2; Humans; Hypertension; Male; Middle Aged; Recombinant Proteins; Thrombin

High contents of Ca2+ was revealed in thrombocytes' intracellular compartments in patients with essential hypertension. In response to different stimuli, large amount of Ca2+ can be released in the platelets of the patients which makes a considerable contribution to generation of the Ca2+ signal and activation of thrombocytes in cardiovascular diseases.

Topics: Blood Platelets; Calcimycin; Calcium; Cell Compartmentation; Cell Membrane; Chlortetracycline; Egtazic Acid; Fluorescent Dyes; Humans; Hypertension; Intracellular Membranes

The investigation was aimed at analyzing membrane-bound calcium in platelets of patients with essential hypertension (EH) and of healthy persons. 55 men were examined. Of these, 38 presented with EH and 17 were healthy. Membrane-bound calcium determined with the help of the chlortetracycline fluorescent probe. The level of membrane-bound calcium in intracellular compartments was higher in patients with EH than in the control. The kinetic curves of the binding of the chlortetracycline fluorescent probe with cellular membranes allow one to reveal disturbances of calcium-dependent membrane processes in persons suffering from EH.

Topics: Adult; Blood Platelets; Calcium; Cell Compartmentation; Cell Membrane; Chlortetracycline; Chronic Disease; Fluorescent Dyes; Humans; Hypertension; Middle Aged

Topics: Animals; Binding Sites; Chlortetracycline; Dogs; Hypertension; Kidney; Male; Nephritis; Oxytetracycline; Rabbits; Rats; Tetracycline

Topics: Anti-Bacterial Agents; Antibiotics, Antitubercular; Chloramphenicol; Chlortetracycline; Erythromycin; Gastroenteritis; Hypertension; Hypokalemia; Pathology; Penicillins; Potassium Deficiency; Pregnancy; Proteinuria; Pyelonephritis