chlortetracycline and Fibrosarcoma

A virus was isolated from a radioresistant feline fibrosarcoma. It induced multi-nucleated giant-cell formation and lysis in a cell line derived from a canine fibro-sarcoma, which was used to characterize the virus. End-point titrations in these cells required 28 days. The virus was sensitive to ether and heat and was destroyed at pH 3. Replication was not inhibited by 5-bromodeoxyuridine. Electron microscopy revealed assembly by a budding process from the plasma membrane of infected cells. The average diameter of the virion was 106 nm. Intracisternal particles with an average diameter of 45 nm were present within infected cells. In two instances secondary monolayers of feline renal cells underwent morphological transformation after inoculation of the virus. The two strains of transformed cells are now in continuous culture and do not yield infectious virus.

Topics: Animals; Bromodeoxyuridine; Cat Diseases; Cats; Cell Line; Chickens; Chloroform; Chlortetracycline; Cytopathogenic Effect, Viral; Depression, Chemical; Dogs; Erythrocytes; Ethyl Ethers; Fibrosarcoma; Filtration; Guinea Pigs; Hemagglutination, Viral; Herpesviridae; Hot Temperature; Humans; Hydrogen-Ion Concentration; Kanamycin; Male; Microscopy, Electron; Oncogenic Viruses; Phosphotungstic Acid; Staining and Labeling; Virus Cultivation; Virus Replication