chlorophyll-a and Metabolic-Diseases

To investigate the effect of hyperoxia on intestinal metabolomics in mice.. Sixteen 8-week-old male C57BL/6 mice were randomly divided into hyperoxia group and control group, with 8 mice in each group. The hyperoxia group was exposed to 80% oxygen for 14 days. Mice were anesthetized and euthanized, and cecal contents were collected for untargeted metabolomics analysis by liquid chromatography-mass spectrometry (LC-MS) combined detection. Orthogonal partial least square discriminant analysis (OPLS-DA), volcano plot analysis, heat map analysis, and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to analyze the effects of hyperoxia on metabolism.. (1) OPLS-DA analysis showed that R. Hyperoxia significantly induces intestinal metabolic disorders. Hyperoxia enhances the metabolism of porphyrins and chlorophyll, inhibits the degradation of lysine, and delays the degradation of aromatic compounds such as benzene and o-cresol.

Topics: Animals; Benzene; Biomarkers; Chlorophyll; Hyperoxia; Lysine; Male; Metabolic Diseases; Mice; Mice, Inbred C57BL; Oxygen; Porphyrins