chlorophyll-a and Dehydration

Topics: Adaptation, Physiological; Chlorophyll; Dehydration; Droughts; Plant Leaves; Populus

Drought stress is one of most dramatic abiotic stresses, reduces crop yield significantly. Application of hormones proved as an effective drought stress ameliorating approach. 24-Epibrassinolide (EBL), an active by-product from brassinolide biosynthesis increases drought stress tolerance in plants significantly. EBL application enhances plant growth and development under drought stress by acting as signalling compound in different physiological processes. This article discussed potential role of 24-epibrassinolide application and drought tolerance in plants. Briefly, EBL sustains or improves plant growth and yield by enhancing carbon assimilation rate, maintaining a balance between ROS and antioxidants and also plays important role in solute accumulation and water relations. Furthermore, we also compared different EBL application methods and concluded that seed priming and foliar application are more productive as compared with root application method. In conclusion, EBL is very impressive phyto-hormone, which can ameliorate drought stress induced detrimental effects in plants.

Topics: Brassinosteroids; Chlorophyll; Dehydration; Oxidation-Reduction; Photosynthesis; Plant Development; Plant Physiological Phenomena; Plants; Steroids, Heterocyclic

Nowadays, the use of the growth regulator salicylic acid for improving a plant's resistance to environmental stresses such as drought is increasing. The present study investigated the effect of salicylic acid on the physiological traits, antioxidant enzymes, yield, and quality of

Topics: Allium; Antioxidants; Chlorophyll; Dehydration; Salicylic Acid; Water

Remote sensing of Solar-induced chlorophyll fluorescence (SIF) has been widely used in estimating Gross Primary Productivity (GPP) and detecting stress in terrestrial ecosystems. Water stress adversely impacts the growth, development, and productivity of a plant. Recently, the characterizing and understanding of the diurnal cycling of plant functioning and ecosystem processes has been explored using SIF. However, the diurnal response of SIF to different levels of water stress remains unclear. This study conducted field experiments on winter wheat by subjecting it to different levels of water stress including well-watered (CK) and, mild, moderate, and severe water stress (D1, D2, D3), and collected the spectral data using an automated SIF measurement system. The results observed the strong SIF-PAR (photosynthetically active radiation) correlations and that these relationships gradually decoupled with increasing water stress, which further decreased the accuracy of temporal upscaling of far-red SIF from an instantaneous to daily scale. To quantify the characteristics of diurnal far-red SIF, five indices including peak time, peak value, curve opening coefficient (leading coefficient of the parabola), and left/right slopes of the peak were proposed. The results demonstrated that diurnal far-red SIF was characterized by an earlier peak time, decreasing peak value, wider curve opening, and flattening right slope from the CK plot to the D3 plot. There were certain mechanisms linking the different indices, for example, between peak size and opening coefficient. Furthermore, the response of far-red SIF to water stress was most pronounced at noon. SIF/PAR exhibited a more significant response to varying water stress compared to far-red SIF, which mitigated the negative influence of PAR variations on diurnal SIF. These findings contribute to the monitoring of plant water dynamics at fine temporal scales.

Topics: Chlorophyll; Dehydration; Ecosystem; Fluorescence; Photosynthesis; Seasons; Triticum

Ledflex is a fluorometer adapted to measure chlorophyll fluorescence at the canopy level. It has been described in detail by Moya et al. (2019), Photosynthesis Research. https://doi.org/10.1007/s11120-019-00642-9 . We used this instrument to determine the effect of water stress on the fluorescence of a fescue field under extreme temperature and light conditions through a 12 days campaign during summer in a Mediterranean area. The fescue field formed part of a lysimeter station in "las Tiesas," near Albacete-Spain. In addition to the fluorescence data, the surface temperature was measured using infrared radiometers. Furthermore, "Airflex," a passive fluorometer measuring the filling-in of the atmospheric oxygen absorption band at 760 nm, was installed in an ultralight plane and flown during the most critical days of the campaign. We observed with the Ledflex fluorometer a considerable decrease of about 53% of the stationary chlorophyll fluorescence level at noon under water stress, which was well correlated with the surface temperature difference between the stressed and control plots. Airflex data also showed a decrease in far-red solar-induced fluorescence upon water stress in agreement with surface temperature data and active fluorescence measurements after correction for PS I contribution. Notwithstanding, the results from airborne remote sensing are not as precise as in situ active data.

Topics: Chlorophyll; Dehydration; Fluorescence; Humans; Photosynthesis; Sunlight

The aim of this study was to determine the drought stress resistance of three chickpea cultivars (Inci, Hasanbey and Seçkin) grown under water deficit conditions and to discuss the use of yield, crop water stress index and chlorophyll index values as drought stress tolerance indicators in breeding studies. Three drought stress levels, (full irrigation = no stress - I

Topics: Chlorophyll; Cicer; Dehydration; Droughts; Plant Breeding

Topics: Bread; Chlorophyll; Dehydration; Droughts; Fluorescence; Photosynthesis; Plant Breeding; Plant Leaves; Triticum

Relay intercropping of legumes with cereals is a useful technique for yield increment. Intercropping may affect the photosynthetic pigments, enzyme activity and yield of barley and chickpea under water stress. To investigate the effect of relay intercropping of barley with chickpea on pigment content, enzyme activity and yield under water stress, a field experiment was conducted during 2017 and 2018. The treatments included irrigation regimes (normal irrigation and cutting off irrigation at the milk development stage) as the main plot. Also, cropping systems as sub plot consisted of sole and relay intercropping of barley with chickpea in two sowing dates (December vs January). Under water stress, the early establishment of barley in December intercropped with chickpea in January (b1c2) enhanced the leaf chlorophyll content by 16% compared to sole cropping due to less competition with chickpea. Late sowing of chickpea enhanced the leaf carotenoid content of chickpea, catalase and peroxidase activities. Barley-chickpea intercropping enhanced the WUE and guaranteed a more efficient use of space (land equivalent ratio of more than 1) compared with sole crops. Under water stress, in b1c2 enhancement of total chlorophyll and water use efficiency caused to increase the grain yield of barley. In b1c2, barley and chickpea reacted to water stress with increasing total chlorophyll and enzyme activity, respectively. In this relay intercropping treatment, each crop occupied and used the growth resources from different ecological niches at different times, which is recommended in semi-arid areas.

Topics: Agriculture; Chlorophyll; Cicer; Dehydration; Edible Grain; Hordeum; Photosynthesis

Since drought stress is one of the key risks for the future of agriculture, exploring the molecular mechanisms of photosynthetic responses to water deficit stress is, therefore, fundamental. By using chlorophyll fluorescence imaging analysis, we evaluated the responses of photosystem II (PSII) photochemistry in young and mature leaves of

Topics: Anthocyanins; Arabidopsis; Chlorophyll; Dehydration; Hormesis; Photosynthesis; Photosystem II Protein Complex; Plant Leaves; Water

Topics: Antioxidants; Chlorophyll; Crops, Agricultural; Dehydration; Photosynthesis; Triticum; Zea mays

Due to the factor of water deficit, which has placed human food security at risk by causing a 20% annual reduction in agricultural products, addressing this growing peril necessitates the adoption of inventive strategies aimed at enhancing plant tolerance. One such promising approach is employing elicitors such as 24-epibrassinolide (EBR) and yeast extract, which are potent agents capable of triggering robust defense responses in plants. By employing these elicitors, crops can develop enhanced adaptive mechanisms to combat water deficit and improve their ability to withstand drought condition. This study investigates the impact of different levels of EBR (0, 5, 10 µm) and yeast extract (0 and 12 g/l) on enhancing the tolerance of cowpea to water deficit stress over two growing seasons.. The findings of this study demonstrate that, the combined application of EBR (especially 10 µm) and yeast extract (12 g/l) can increase seed yield (18%), 20-pod weight (16%), the number of pods per plant (18%), total chlorophyll content (90%), and decrease malondialdehyde content (45%) in cowpea, compared to plants grown under water deficit stress without these treatments. Upon implementing these treatments, impressive results were obtained, with the highest recorded values observed for the seed yield (1867.55 kg/ha), 20-pod weight (16.29 g), pods number per plant (9), and total chlorophyll content (19.88 mg g. The comprehensive exploration of the effects of EBR and yeast extract across various levels on cowpea plants facing water deficit stress presents a pivotal contribution to the agricultural domain. This research illuminates a promising trajectory for future agricultural practices and users seeking sustainable solutions to enhance crops tolerance. Overall, the implications drawn from this study contribute significantly towards advancing our understanding of plant responses to water deficit stress while providing actionable recommendations for optimizing crop production under challenging environmental conditions.

Topics: Chlorophyll; Dehydration; Humans; Plant Breeding; Plants; Vigna; Water

The growth and productivity of plants are enhanced by the use of thiourea (TU) under stressful conditions. When TU is applied as a rooting medium, it improves plant growth characteristics and other physiological parameters in stressed environment. A pot experiment was conducted in the botanical garden of the Government College University, Faisalabad 38000, Pakistan to examine the TU-mediated fluctuations in some crucial physio-biochemical parameters and the oxidative defense of potatoes under a restricted water supply. For this purpose, two potato cultivars (potato-SH-5 and potato-FD-73) were sown in pots containing 10 kg of soil. Water was regularly applied to the pots until germination. After 2 weeks of germination, drought stress with 65% field capacity was imposed, while the control was subjected to 100% field capacity. TU, as a rooting medium, was applied at the vegetative stage (0 (no application), 0.5, 0.75 mM). A substantial reduction in the total number of leaves, leaf area, tuber biomass (fresh and dry weight), photosynthetic pigments, membrane permeability, and leaf relative water content (RWC) was recorded in plants under drought stress conditions as compared to control plants. The damaging effects of water stress were more critical for cv. potato-FD-73 as compared to cv. potato-SH-5. In contrast, drought stress enhanced the malondialdehyde (MDA) and hydrogen peroxide (H

Topics: Antioxidants; Chlorophyll; Dehydration; Droughts; Hydrogen Peroxide; Oxidative Stress; Peroxidase; Peroxidases; Solanum tuberosum; Sugars; Superoxide Dismutase

Photosynthesis regulation is fundamental for the response to environmental dynamics, especially for bryophytes during their adaptation to terrestrial life. Alternative electron flow mediated by flavodiiron proteins (FLV) and cyclic electron flow (CEF) around photosystem I (PSI) play seminal roles in the response to abiotic stresses in mosses; nevertheless, their correlation and relative contribution to photoprotection of mosses exposed to combined stresses remain unclear. In the present study, the photosynthetic performance and recovery capacity of three moss species from different growth habitats were examined during heat and dehydration with fluctuating light. Our results showed that dehydration at 22 °C for 24 h caused little photodamage, and most of the parameters recovered to their original values after rehydration. In contrast, dehydration at 38 °C caused drastic injuries, especially to PSII, which was mainly caused by the inactivation of non-photochemical quenching (NPQ). Dehydration also induced a high accumulation of O

Topics: Bryophyta; Chlorophyll; Dehydration; Ecosystem; Electron Transport; Hot Temperature; Hydrogen Peroxide; Light; Photosynthesis; Photosystem I Protein Complex; Photosystem II Protein Complex; Plant Breeding; Plant Leaves; Temperature

Drought stress is among the most serious threats jeopardizing the economic yield of crop plants in Iran. In particular, in response to withholding irrigation, the reduction in performance and quality of a precious plant such as the olive tree is remarkable. Therefore, the selection of cultivars that are resistant or tolerant to drought has been recognized as one of the most effective long-term strategies for sustainably alleviating the adverse effects of this stress. In this view, our study evaluated the response of 8 olive cultivars including 4 elite native cultivars (Zard Aliabad, Roughani, Dezful, and Shengeh) and 4 foreign cultivars (Manzanilla, Sevillana, Konservolia, and Mission) to water shortage in the Dallaho Olive Research station of Sarpole-Zahab in Kermanshah province in 2020. Olive trees underwent 3 levels of irrigation treatment including 100% full irrigation (control), 75%, and 50% deficit irrigation.. Based on the results, 50% deficit irrigation decreased both growth and pomological traits, but determined the highest dry matter percentage. As the severity of drought stress increased, with an accumulation of sodium and malondialdehyde, an incremental increase in osmolytes was observed, as well as an enhancement of the activity of antioxidant enzymes (peroxidase and catalase). In contrast, full irrigation led to an increase in photosynthetic pigments, calcium, and potassium. Dezful and Konservolia cultivars revealed a significantly higher growth rate, correlated in the former to higher levels of chlorophyll, compatible compounds, total phenolic content, relative water content, potassium to sodium ratio, catalase, and peroxidase activities compared with other cultivars. Konservolia showed the best yield parameters under 75% and 100% irrigation regimes, correlated to higher chlorophyll, potassium, and total phenolic content (in particular at 75% ET).. Generally, the selection of more resilient or tolerant cultivars to sustain water scarcity stress is a widely operative solution to extend rainfed orchards in semi-arid environments. Our study showed that Dezful and Konservolia had the best adaptive mechanisms to cope with the detrimental effects of drought stress.

Topics: Antioxidants; Calcium; Catalase; Chlorophyll; Dehydration; Malondialdehyde; Olea; Peroxidases; Phenols; Potassium; Sodium; Water

Strawberry (Fragaria × ananassa Duch) plants are vulnerable to climatic change. The strawberry plants suffer from heat and water stress eventually, and the effects are reflected in the development and yields. In this investigation, potential chlorophyll-fluorescence-based indices were selected to detect the early heat and water stress in strawberry plants. The hyperspectral images were used to capture the fluorescence reflectance in the range of 500 nm-900 nm. From the hyperspectral cube, the region of interest (leaves) was identified, followed by the extraction of eight chlorophyll-fluorescence indices from the region of interest (leaves). These eight chlorophyll-fluorescence indices were analyzed deeply to identify the best indicators for our objective. The indices were used to develop machine-learning models to assess the performance of the indicators by accuracy assessment. The overall procedure is proposed as a new workflow for determining strawberry plants' early heat and water stress. The proposed workflow suggests that by including all eight indices, the random-forest classifier performs well, with an accuracy of 94%. With this combination of the potential indices, namely the red-edge vegetation stress index (RVSI), chlorophyll B (Chl-b), pigment-specific simple ratio for chlorophyll B (PSSR

Topics: Chlorophyll; Dehydration; Fluorescence; Fragaria; Hot Temperature

Drought stress severely limits plant growth and production in apple (Malus domestica Borkh.). To breed water-deficit-tolerant apple cultivars that maintain high yields under slight or moderate drought stress, it is important to uncover the mechanisms underlying the transcriptional regulation of chlorophyll metabolism in apple. To explore this mechanism, we generated transgenic 'Gala3' apple plants with overexpression or knockdown of MdWRKY17, which encodes a transcription factor whose expression is significantly induced by water deficit. Under moderate drought stress, we observed significantly higher chlorophyll contents and photosynthesis rates in overexpression transgenic plants than in controls, whereas these were dramatically lower in the knockdown lines. MdWRKY17 directly regulates MdSUFB expression, as demonstrated by in vitro and in vivo experiments. MdSUFB, a key component of the sulfur mobilization (SUF) system that assembles Fe-S clusters, is essential for inhibiting chlorophyll degradation and stabilizing electron transport during photosynthesis, leading to higher chlorophyll levels in transgenic apple plants overexpressing MdWRKY17. The activated MdMEK2-MdMPK6 cascade by water-deficit stress fine-tunes the MdWRKY17-MdSUFB pathway by phosphorylating MdWRKY17 under water-deficit stress. This fine-tuning of the MdWRKY17-MdSUFB regulatory pathway is important for balancing plant survival and yield losses (chlorophyll degradation and reduced photosynthesis) under slight or moderate drought stress. The phosphorylation by MdMEK2-MdMPK6 activates the MdWRKY17-MdSUFB pathway at S66 (identified by LC-MS), as demonstrated by in vitro and in vivo experiments. Our findings reveal that the MdMEK2-MdMPK6-MdWRKY17-MdSUFB pathway stabilizes chlorophyll levels under moderate drought stress, which could facilitate the breeding of apple varieties that maintain high yields under drought stress.

Topics: Chlorophyll; Dehydration; Droughts; Gene Expression Regulation, Plant; Malus; MAP Kinase Kinase 2; Metabolic Networks and Pathways; Mitogen-Activated Protein Kinases; Phosphorylation; Photosynthesis; Phylogeny; Plant Proteins; Plants, Genetically Modified; Transcription Factors

Cytokinins play a central role in delaying senescence, reducing oxidative damage and maintaining plant growth during drought. This study showed that the ectopic expression of ProRE-deleted MdIPT5b, a key enzyme involved in cytokinin metabolism, increased the drought tolerance of transgenic Malus domestica (apple) callus and Solanum lycopersicum (tomato) seedlings by maintaining cytokinin homeostasis, and thus maintaining redox balance. Under restricted watering regimes, the yields of transgenic tomato plants were enhanced. Heterodimers of C/S1 bZIP are involved in the cytokinin-mediated drought response. The heterodimers bind the ProRE of MdIPT5b promoter, thus directly suppressing gene transcription. Single C/S1 bZIP members could not independently function as suppressors. However, specific paired members (heterodimers of MdbZIP80 with MdbZIP2 or with MdbZIP39) effectively suppressed transcription. The α-helical structure is essential for the heterodimerization of C/S1 bZIP members and for synergistic transcriptional suppression. As negative regulators of drought tolerance, suppressing either MdbZIP2 or MdbZIP39 alone does not improve the expression of MdIPT5b and did not increase the drought tolerance of transgenic apple callus. However, this could be achieved when they were co-suppressed. The suppression of MdbZIP80 alone could improve MdIPT5b expression and increase the drought tolerance of transgenic apple callus. However, these effects were reversed in response to the cosuppression of MdbZIP80 and MdIPT5b. Similar results were also observed during delayed dark-induced senescence in apple leaves. In conclusion, the apple C/S1 bZIP network (involving MdbZIP2, MdbZIP39 and MdbZIP80) directly suppressed the expression of MdIPT5b, thus negatively modulating drought tolerance and dark-induced senescence in a functionally redundant manner.

Topics: Basic-Leucine Zipper Transcription Factors; Cellular Senescence; Chlorophyll; Cytokinins; Dehydration; Gene Expression Regulation, Plant; Homeostasis; Malus; Oxidation-Reduction; Plant Leaves; Plant Proteins; Plants, Genetically Modified; Real-Time Polymerase Chain Reaction; Seedlings; Solanum lycopersicum

Organic mulches are widely used in crop production systems. Due to their benefits in improving soil fertility, retention of soil moisture and weed control. Field experiments were conducted during wheat growing seasons of 2018-2019 and 2019-2020 to evaluate the effects of Jatropha leaves mulch on the growth of wheat varieties 'Wadan-17' (rainfed) and 'Pirsabaq-2013' (irrigated) under well irrigated and water stress conditions (non-irrigated maintaining 40% soil field capacity). Jatropha mulch was applied to the soil surface at 0, 1, 3 and 5 Mg ha. We noted a decrease in plant height, shoot and root fresh/dry weight, leaf area, leaf relative water content (LRWC), chlorophyll, and carotenoid content due to water stress. However, water stress caused an increase in leaf and root phenolics content, leaf soluble sugars and electrolytes leakage. We observed that Jatropha mulch maintained LRWC, plant height, shoot and root fresh/dry weight, leaf area and chlorophyll content under water stress. Moreover, water stress adverse effects on leaf soluble sugar content and electrolyte leakage were reversed to normal by Jatropha mulch.. Therefore, it may be concluded that Jatropha leaves mulch will minimize water stress adverse effects on wheat by maintaining soil moisture and plant water status.

Topics: Carbohydrate Metabolism; Carotenoids; Chlorophyll; Crop Production; Dehydration; Jatropha; Plant Leaves; Plant Roots; Soil; Triticum

Chlorophyll fluorescence (ChlF) is a powerful non-invasive technique for probing photosynthesis. Although proposed as a method for drought tolerance screening, ChlF has not yet been fully adopted in physiological breeding, mainly due to limitations in high-throughput field phenotyping capabilities. The light-induced fluorescence transient (LIFT) sensor has recently been shown to reliably provide active ChlF data for rapid and remote characterisation of plant photosynthetic performance. We used the LIFT sensor to quantify photosynthesis traits across time in a large panel of durum wheat genotypes subjected to a progressive drought in replicated field trials over two growing seasons. The photosynthetic performance was measured at the canopy level by means of the operating efficiency of Photosystem II (

Topics: Chlorophyll; Dehydration; Electron Transport; Genetic Association Studies; Genetic Variation; Photosynthesis; Photosystem II Protein Complex; Quantitative Light-Induced Fluorescence; Quantitative Trait, Heritable; Triticum

Nano-titanium dioxide (nTiO

Topics: Chlorophyll; Dehydration; Hydrogen Peroxide; Lipid Peroxidation; Nanoparticles; Nitric Oxide; Stress, Physiological; Superoxides; Titanium; Vicia faba

Climate change affecting on weather in many different ways especially drought and temperature rise. This will drastically down plant production, if not start looking for another source to increase water productivity to cope up with water stress conditions. In this study efforts were conducted to interpret the use of anti-transpirants to conserving irrigation water, aiding plant survival under dry conditions and protecting plant against drought stress.. Two field experiments were carried out during 2017 and 2018 successive growing summer seasons at the experimental farm of National Research Centre, Nubaria, El-Beheira Governorate, Egypt. Anti-transparent ,i.e., chitosan (100 and 150 mg L-1), calcium carbonate (5 and 10 g L-1), salicylic acid (200 and 300 mg L-1) were foliar sprayed on sunflower plants grown under two water levels (normal 100% and deficit 50%).. The results showed that water stress decreased growth criteria, photosynthetic pigments, osmoprotectants, yield components, oil and carbohydrate (%) as compared to 100% of irrigation requirements. Meanwhile, water deficit induced significant increases in (proline). Foliar treatments of sunflower plant with chitosan, calcium carbonate, salicylic acid increased growth criteria, yield components, photosynthetic pigments, total soluble sugars, proline and free amino acid as compared to control plant. Data also illustrated that, all used treatment improved seed yield, oil and carbohydrate% of sunflower plants.. Generally, it could be concluded that 10 g L-1 CaCO3 and 300 mg L-1 SA at 50% level of water irrigation could be recommended for sunflower plant grown under similar field conditions in order to get optimum yield and to save irrigation water.

Topics: Agricultural Irrigation; Calcium Carbonate; Carbohydrate Metabolism; Chitosan; Chlorophyll; Crops, Agricultural; Dehydration; Droughts; Egypt; Helianthus; Oxidative Stress; Photosynthesis; Proline; Salicylic Acid; Seasons; Water

As functional proteins, dehydrins are found in many maturing seeds and vegetable tissues under adverse environmental conditions. However, the regulation of dehydrin expression remains unclear.. In this study, a novel drought stress-related bHLH transcription factor, TabHLH49, was isolated from a wheat cDNA library treated with the drought and cold stress by using yeast one-hybrid system. TabHLH49 protein possesses a typical conserved bHLH domain and is a Myc-type bHLH transcription factor. TabHLH49 was detected in the nucleus of tobacco epidermal cells, and the amino acid sequences at the C-terminus (amino acids 323-362) is necessary for its transactivation activity. Real-time PCR analyses revealed the tissue-specific expression and drought stress-responsive expression of TabHLH49 in wheat. In addition, the verification in Y1H and electrophoretic mobility shift assays illustrated that TabHLH49 protein can bind and interact with the promoter of the wheat WZY2 dehydrin. Furthermore, the dual-luciferase assays showed that TabHLH49 can positively regulate the expression of WZY2 dehydrin. The transient expression and BSMV-mediated gene silencing of TabHLH49 also showed that TabHLH49 positively regulates the expression of WZY2 dehydrin and improves drought stress resistance in wheat.. These results provide direct evidences that TabHLH49 positively regulates expression level of dehydrin WZY2 gene and improves drought tolerance of wheat.

Topics: Chlorophyll; Dehydration; Gene Expression Regulation, Plant; Malondialdehyde; Plant Proteins; Real-Time Polymerase Chain Reaction; Transcription Factors; Transcriptome; Triticum; Two-Hybrid System Techniques

Sugarcane transgenic overexpressing EaGly III from Erianthus arundinaceus showed enhanced water deficit stress tolerance. Methylglyoxal (MG), an α-ketoaldehyde formed from either glycolysis or TCA cycle, is capable of causing total cellular damage via the generation of reactive oxygen species (ROS), advanced glycation end products (AGEs) and nucleic acid degradation. Glyoxalase pathway is a ubiquitous pathway known for detoxification of MG, involving key enzymes glyoxalase I (Gly I) and glyoxalase II (Gly II). Recently, a novel and an additional enzyme in glyoxalase pathway, viz., glyoxalase III (Gly III), has been discovered which possesses DJ-1/PfpI domain recognized for detoxifying MG in a single step process without requirement of any coenzyme. In the present study, a Gly III gene isolated from Erianthus arundinaceus, a wild relative of sugarcane, overexpressed in commercially cultivated sugarcane hybrid Co 86032 was assessed for drought tolerance. Morphometric observations revealed that transgenic sugarcane overexpressing EaGly III acquired drought tolerance trait. Oxidative damage caused by triggering generation of ROS has been determined to be low in transgenic plants as compared to wild type (WT). Transgenics resulted in higher relative water content, chlorophyll content, gas exchange parameters, photosynthetic efficiency, proline content and soluble sugars upon water deficit stress. In addition, higher and stable level of superoxide dismutase and peroxidase activities were observed along with minimal lipid peroxidation during drought stress signifying the tolerance mechanism exhibited by transgenic events. There was no significant structural change observed in the root anatomy of transgenic plants. Altogether, EaGly III gene could be considered as a potential candidate for conferring water deficit stress tolerance for sugarcane and other agricultural crops.

Topics: Aldehyde Oxidoreductases; Carotenoids; Cell Membrane; Chlorophyll; Dehydration; Droughts; Ectopic Gene Expression; Gene Expression Regulation, Plant; Plant Proteins; Plant Roots; Plants, Genetically Modified; Proline; Saccharum; Sugars

Drought stress is the most harmful one among other abiotic stresses with negative impacts on crop growth and development. Drought-hardening is a feasible and widely used method in tobacco seedlings cultivation. It has gained extensive interests due to its role in improving drought tolerance. This research aimed to investigate the role of drought-hardening and to unravel the multiple mechanisms underlying tobacco drought tolerance and adaptation.. This study was designed in which various drought-hardening treatments (CK (no drought-hardening), T1 (drought-hardening for 24 h), T2 (drought-hardening for 48 h), and T3 (drought-hardening for 72 h)) were applied to two tobacco varieties namely HongHuaDaJinYuan (H) and Yun Yan-100 (Y). The findings presented a complete framework of drought-hardening effect at physiological, biochemical, and gene expression levels of the two tobacco varieties under drought stress. The results showed that T2 and T3 significantly reduced the growth of the two varieties under drought stress. Similarly, among the various drought-hardening treatments, T3 improved both the enzymatic (POD, CAT, APX) and non-enzymatic (AsA) defense systems along with the elevated levels of proline and soluble sugar to mitigate the negative effects of oxidative damage and bringing osmoregulation in tobacco plants. Finally, the various drought-hardening treatments (T1, T2, and T3) showed differential regulation of genes expressed in the two varieties, while, particularly T3 drought-hardening treatment-induced drought tolerance via the expression of various stress-responsive genes by triggering the biosynthesis pathways of proline (P5CS1), polyamines (ADC2), ABA-dependent (SnRK2, AREB1), and independent pathways (DREB2B), and antioxidant defense-related genes (CAT, APX1, GR2) in response to drought stress.. Drought-hardening made significant contributions to drought tolerance and adaptation in two tobacco variety seedlings by reducing its growth and, on the other hand, by activating various defense mechanisms at biochemical and molecular levels. The findings of the study pointed out that drought-hardening is a fruitful strategy for conferring drought tolerance and adaptations in tobacco. It will be served as a useful method in the future to understand the drought tolerance and adaptation mechanisms of other plant species. Drought-hardening improved drought tolerance and adaptation of the two tobacco varieties. T1 indicates drought-hardening for 24 h, T2 indicates drought-hardening for 48 h, T3 indicates drought-hardening for 72 h.

Topics: Adaptation, Physiological; Ascorbic Acid; Chlorophyll; Crop Production; Dehydration; Fluorescence; Gene Expression Regulation, Plant; Glutathione; Hydrogen Peroxide; Malondialdehyde; Nicotiana; Oxidation-Reduction; Plant Leaves; Proline

Cuticular wax plays important role in protecting plants from drought stress. In Arabidopsis WRI4 improves drought tolerance by regulating the biosynthesis of fatty acids and cuticular wax. Cyperus esculentus (yellow nutsedge) is a tough weed found in tropical and temperate zones as well as in cooler regions. In the current study, we report the molecular cloning of a WRI4-like gene from Cyperus esculentus and its functional characterization in Arabidopsis.. Using RACE PCR, full-length WRI-like gene was amplified from yellow nutsedge. Phylogenetic analyses and amino acid comparison suggested it to be a WRI4-like gene. According to the tissue-specific expression data, the highest expression of WRI4-like gene was found in leaves, followed by roots and tuber. Transgenic Arabidopsis plants expressing nutsedge WRI4-like gene manifested improved drought stress tolerance. Transgenic lines showed significantly reduced stomatal conductance, transpiration rate, chlorophyll leaching, water loss and improved water use efficiency (WUE). In the absence of drought stress, expression of key genes for fatty acid biosynthesis was not significantly different between transgenic lines and WT while that of cuticular wax biosynthesis genes was significantly higher in transgenic lines than WT. The PEG-simulated drought stress significantly increased expression of key genes for fatty acid as well as wax biosynthesis in transgenic Arabidopsis lines but not in WT plants. Consistent with the gene expression data, cuticular wax load and deposition was significantly higher in stem and leaves of transgenic lines compared with WT under control as well as drought stress conditions.. WRI4-like gene from Cyperus esculentus improves drought tolerance in Arabidopsis probably by promoting cuticular wax biosynthesis and deposition. This in turn lowers chlorophyll leaching, stomatal conductance, transpiration rate, water loss and improves water use efficiency under drought stress conditions. Therefore, CeWRI4-like gene could be a good candidate for improving drought tolerance in crops.

Topics: Arabidopsis; Chlorophyll; Cyperus; Dehydration; Fatty Acids; Genes, Plant; Phylogeny; Plant Epidermis; Plant Leaves; Plant Proteins; Plant Transpiration; Plants, Genetically Modified; Real-Time Polymerase Chain Reaction; Waxes

Water deficiency up to a certain level and duration leads to a stress condition called drought. It is a multi-dimensional stress causing alteration in the physiological, morphological, biochemical, and molecular traits in plants resulting in improper plant growth and development. Drought is one of the major abiotic stresses responsible for loss of crops including muskmelon (Cucumis melo. L). Muskmelon genotype SC-15, which exhibits high drought resistance as reported in our earlier reports, was exposed to deficient water condition and studied for alteration in physiological, molecular and proteomic profile changes in the leaves. Drought stress results in reduced net photosynthetic rate (Pn), stomatal conductance (Gs) and transpiration (E) rate. With expanded severity of drought, declination recorded in content of total chlorophyll and carotenoid while enhancement observed in phenol content indicating generation of oxidative stress. In contrary, activities of catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX), and guaiacol (POD) were increased under drought stress. Peptide mass fingerprinting (PMF) showed that drought increased the relative abundance of 38 spots while decreases10 spots of protein. The identified proteins belong to protein synthesis, photosynthesis, nucleotide biosynthesis, stress response, transcription regulation, metabolism, energy and DNA binding. A drought-induced MADS-box transcription factor was identified. The present findings indicate that under drought muskmelon elevates the abundance of defense proteins and suppresses catabolic proteins. The data obtained exhibits possible mechanisms adopted by muskmelon to counter the impacts of drought induced stress.

Topics: Chlorophyll; Cucumis melo; Dehydration; Droughts; Electrophoresis, Gel, Two-Dimensional; Gene Expression Regulation, Plant; Photosynthesis; Plant Proteins; Plant Transpiration; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Transcriptome

Guzmania monostachia is an epiphytic tank bromeliad that displays the inducible CAM photosynthesis under stressful conditions and had the highest stomata density in the leaf apex, while the base portion has the highest density of trichomes, which are specialized structures used to acquire water and nutrients from the tank solution. In order to correlate the genetic factors behind these morpho-physiological characteristics along the leaf blade of G. monostachia, a comparative transcriptome analysis was performed to identify the functional enriched pathways and unigenes that could play a role in the apical, middle and basal leaf portions. A total of 653 million reads were used for de novo transcriptome assembly, resulting in 48,051 annotated unigenes. Analysis of differentially expressed genes (DEGs) among distinct leaf regions revealed that 806 DEGs were upregulated in the apex compared to the middle portion, while 9685 DEGs were upregulated in the apex and 9784 DEGs were upregulated in the middle portions compared to the base. Our outcomes correlated some DEGs and identified unigenes with their physiological functions, mainly suggesting that the leaf apex was related to the regulation of stomatal movement, production of chlorophyll, cellular response to stress, and H2O2 catabolic process. In contrast, the middle portion showed DEGs associated with the transport of amino acids. Furthermore, DEGs from the leaf base were mainly correlated with responses to nutrients and nitrogen compounds, regulation of potassium ion import, response to water deprivation, and trichome branching, indicating that, at least in part, this leaf portion can replace some of the root functions of terrestrial plants. Therefore, possibly candidate unigenes and enriched pathways presented here could be prospected in future experimental work, opening new possibilities to bioengineer non-inducible CAM plants and/or improve the fertilization use efficiency by increasing leaf nutrient acquisition of crop plants.

Topics: Bromelia; Bromeliaceae; Chlorophyll; Dehydration; Droughts; Gene Expression Profiling; Gene Expression Regulation, Plant; Photosynthesis; Plant Leaves; Transcriptome; Water

Little is known about mechanisms of sweet briar adaptation to dry habitats. The species is highly invasive and displaces native plants from dry lands of the southern hemisphere. This study evaluates physiological basis of Rosa rubiginosa L. adaptation to soil drought. We performed a pot soil drought experiment and assessed water relations, water use efficiency, gas exchange and photosynthetic apparatus activity. The study also measured the content of chlorophyll, soluble carbohydrates and proline and analyzed plant biomass growth. We hypothesized that the drought stress induced an effective mechanism enabling adaptation of young sweet briar roses to soil water deficit. The study identified several adaptation mechanisms of R. rubiginosa allowing the plant to survive soil drought. These included limiting transpiration and stomatal conductance, increasing the level of soluble sugars, reducing chlorophyll content, accumulating CO

Topics: Adaptation, Physiological; Chlorophyll; Dehydration; Droughts; Ecosystem; Photosynthesis; Plant Leaves; Rosa; Water

Understanding the relationship between exogenous melatonin and water deficit stress is crucial for achieving high yields and alleviating the effects of water deficit stress on soybean (Glycine max (L.) Merrill) plants in agriculture. This study investigated the effects of exogenous melatonin on soybean photosynthetic capacity under water deficit stress induced by polyethylene glycol (PEG) 6000. We conducted a potting experiment in 2018 using the soybean (Glycine max L. Merrill) cultivar Suinong 26. We identified the impacts of a concentration of PEG 6000 simulating drought (15%, w/v) and an appropriate melatonin concentration (100 μmol/L) on the growth of soybean seedlings and flowering stages in a preliminary test. We applied exogenous melatonin by foliar spraying and root application to determine the effects on leaf photosynthesis during water deficit stress. Our results indicated that 15% PEG 6000 had an obvious inhibitory effect on the growth of soybean seedlings and flowering stages, causing oxidative stress and damage due to reactive oxygen species (ROS) (H2O2 and O2·-) accumulation and potentially reducing air exchange parameters and photosystem II (PSII) efficiency. The application of exogenous melatonin significantly relieved the inhibitory effects of PEG 6000 stress on seedlings and flowering growth, and gas exchange parameters, potentially improved PSII efficiency, improved the leaf area index (LAI) and the accumulation of dry matter, slowed down oxidative stress and damage to leaves by increasing the activity of antioxidant enzymes (SOD, POD, and CAT), reduced the content of malondialdehyde (MDA), and ultimately improved soybean yield. Overall, the results of this study demonstrated that application of exogenous melatonin at the seedlings and flowering stages of soybean is effective in alleviating plant damage caused by water deficit stress and improving the drought resistance of soybean plants. In addition, the results showed that application of exogenous melatonin by root is superior to foliar spraying.

Topics: Antioxidants; Chlorophyll; Dehydration; Down-Regulation; Droughts; Glycine max; Lipid Peroxidation; Melatonin; Osmotic Pressure; Oxidative Stress; Photosynthesis; Preliminary Data; Seedlings; Stress, Physiological

Density and length of leaf pubescence are important factors of diversity in the response to water deficiency among wheat genotypes. Many studies evidence an important protective value of leaf hairiness in plants, especially under the conditions of drought, thermal loads and increased solar radiation. However, the physiological and adaptive roles of such traits in cereals, including cultivated plants, have not been sufficiently studied to date. The aim of this work was to study the association of morphological characteristics of leaves with parameters of gas exchange and chlorophyll fluorescence in wheat lines carrying a genetically different leaf hairiness. Isogenic and inter-varietal substitution wheat lines were used, carrying various combinations of dominant and recessive alleles of the known genes. A quantitative assessment of the pubescence was carried out in contrasting watering conditions to establish the physiological role of this trait in adaptation to drought. With the help of a portable system for studying the gas exchange and chlorophyll fluorescence, ten parameters of photosynthesis were studied, as well as morphological features of leaves and shoot biomass. It was found that gas exchange parameters are inversely proportional to the density and length of trichomes. In drought conditions, the trichome density increased and the length of trichomes decreased under the observed decrease in the level of gas exchange. A similar dependence was observed for the level of non-photochemical quenching of chlorophyll fluorescence. Under optimal conditions, the poorly haired cultivars exhibited a higher biomass than the densely haired. However, under water deficiency they significantly reduced the biomass and showed a low value of the tolerance index.

Topics: Chlorophyll; Dehydration; Photosynthesis; Plant Leaves; Quantitative Trait, Heritable; Triticum

Multiple dehydration/rehydration treatments improve the adaptation of Craterostigma plantagineum to desiccation by accumulating stress-inducible transcripts, proteins and metabolites. These molecules serve as stress imprints or memory and can lead to increased stress tolerance. It has been reported that repeated exposure to dehydration may generate stronger reactions during a subsequent dehydration treatment in plants. This stimulated us to address the question whether the desiccation tolerant resurrection plant Craterostigma plantagineum has a stress memory. The expression of four representative stress-related genes gradually increased during four repeated dehydration/rehydration treatments in C. plantagineum. These genes reflect a transcriptional memory and are trainable genes. In contrast, abundance of chlorophyll synthesis/degradation-related transcripts did not change during dehydration and remained at a similar level as in the untreated tissues during the recovery phase. During the four dehydration/rehydration treatments the level of ROS pathway-related transcripts, superoxide dismutase (SOD) activity, proline, and sucrose increased, whereas H

Topics: Adaptation, Physiological; Chlorophyll; Craterostigma; Dehydration; Gene Expression Regulation, Plant; Hydrogen Peroxide; Lipid Peroxidation; Proline; Reverse Transcriptase Polymerase Chain Reaction

Drought stress is a serious threat to agriculture and the environment. Brassinosteroids (BRs) increase tolerance to drought stress of plant. Autophagy plays important roles in plant responses to drought stress; however, there are few reports on autophagy in peach (Prunus persica). In total, 23 putative autophagy-related genes (ATGs) in peach were identified using ATGs from the Arabidopsis thaliana genome as query in BLASTx algorithm-based searches. Under drought stress, the photosynthetic abilities of peach leaves decreased, while antioxidant enzyme activities, autophagy and ATG expression increased. A correlation analysis showed that antioxidant enzyme activities are inversely correlated to the expression levels of the PpATGs. During drought, the PpATG8s and some PpATG18s had the strongest responses. To investigate enhanced drought-stress tolerance, peach was treated with water, 100 nM 24-epibrassinolide (EBR), 1 μM EBR, 10 μM EBR and 1 μM voriconazole. Exogenous EBR at 1 μM decreased the malondialdehyde (MDA) content under drought stress when compared with water-, 1 μM voriconazole-, 100 nM EBR- and 10 μM EBR-treated peach leaf. The 1-μM EBR application increased superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX) and glutathione peroxidase (GR) activities during drought stress. In addition, the expression levels of PpATGs were inhibited by EBR. Thus, the 1-μM EBR treatment alleviated drought-stress damage to peach leaves, decreased PpATG expression levels and reduced the number of autophagosomes.

Topics: Arabidopsis; Ascorbate Peroxidases; Autophagosomes; Autophagy; Brassinosteroids; Catalase; Chlorophyll; Dehydration; Gene Expression Regulation, Plant; Glutathione Peroxidase; Microscopy, Electron; Peroxidase; Photosynthesis; Plant Leaves; Plant Transpiration; Prunus persica; Steroids, Heterocyclic; Superoxide Dismutase

Nitrogen (N) allocation in leaves affects plant photosynthesis-N relationship and adaptation to environmental fluctuations. To reveal the role of leaf N allocation in water deficit stress acclimation in rice, the plants were grown in infertile soil supplying with low N (0.05 g N·kg

Topics: Acclimatization; Amino Acids; Catalase; Chlorophyll; Dehydration; Malondialdehyde; Nitrogen; Oryza; Photosynthesis; Plant Leaves; Proline

Drought is one of the major abiotic stress factors that affect grape growth and yield, which in turn negatively affects the grape and wine production industry. Developing effective approaches to improve grapevine tolerance to drought stress is a priority for viticulture. Strigolactones, a newly discovered class of carotenoid-derived phytohormones, have been found to participate in various physiological processes. Herein, the effect of strigolactones (SLs) on grape seedlings under drought stress was investigated. Two-year-old grape seedlings (Vitis vinifera L.) were sprayed with 3 doses of rac-GR24 (1 μM, 3 μM and 5 μM), a synthesized strigolactone, and then were subjected to 7% (w/v) polyethylene glycol (PEG-6000) to simulate the drought conditions. Synthetic GR24 treated plants showed higher tolerance to drought stress with regard to lower electrolyte leakage, stomatal opening, reactive oxygen species (ROS), and higher relative water content, chlorophyll content, photosynthesis rate and malondialdehyde (MDA) content. GR24 application also decreased the levels of indoleacetic acid (IAA) and zeatin riboside (ZR), while increasing the level of abscisic acid (ABA), both in the roots and leaves under drought stress. These results suggested that foliar application of GR24 could ameliorate the adverse effects of drought due to its regulation of stomatal closure through ABA or ROS, and modulation of chlorophyll components and photosynthesis, as well as activation of the antioxidant defense capacity. Cross-talk with other hormones, especially ABA, was also suggested to be one of the important mechanism during this process. This study contributes to our current understanding of GR24-induced drought tolerance in grapevines.

Topics: Abscisic Acid; Chlorophyll; Dehydration; Heterocyclic Compounds, 3-Ring; Lactones; Photosynthesis; Plant Growth Regulators; Plant Leaves; Plant Stomata; Vitis

Protective compounds such as non-enzymatic antioxidants, osmolytes and signal molecules have been applied to plants exposed to various environmental stresses to increase their stress tolerance. However, there are not enough records about the response of plants to alpha lipoic acid (ALA) application with antioxidant properties. Therefore, this study was designed to evaluate the function of exogenous ALA on the photosynthetic performance of maize seedlings grown in hydroponic conditions under drought stress. Three weeks old seedlings were treated with or without ALA (12 μM) and they were subjected to drought stress induced by 10% polyethylene glycol (PEG

Topics: Blotting, Western; Carbon Cycle; Chlorophyll; Dehydration; Gene Expression Regulation, Plant; Lipid Peroxidation; Photosystem II Protein Complex; Plant Leaves; Real-Time Polymerase Chain Reaction; Seedlings; Thioctic Acid; Zea mays

A 45-days long climatic chamber experiment was design to evaluate the effect of 400 and 700 ppm atmospheric CO

Topics: Antioxidants; Carbon Dioxide; Chenopodiaceae; Chlorophyll; Dehydration; Fatty Acids; Oxidation-Reduction; Photosynthesis; Salt Stress; Soil

Cotton GaTOP6B is involved in cellular endoreduplication and a positive response to drought stress via promoting plant leaf and root growth. Drought is deemed as one of adverse conditions that could cause substantial reductions in crop yields worldwide. Since cotton exhibits a moderate-tolerant phenotype under water-deficit conditions, the plant could therefore be used to characterize potential new genes regulating drought tolerance in crop plants. In this work, GaTOP6B, encoding DNA topoisomerase VI subunit B, was identified in Asian cotton (Gossypium arboreum). Virus-induced gene silencing (VIGS) and overexpression (OE) were used to investigate the biological function of GaTOP6B in G. arboreum and Arabidopsis thaliana under drought stress. The GaTOP6B-silencing plants showed a reduced ploidy level, and displayed a compromised tolerance phenotype including lowered relative water content (RWC), decreased proline content and antioxidative enzyme activity, and an increased malondialdehyde (MDA) content under drought stress. GaTOP6B-overexpressing Arabidopsis lines, however, had increased ploidy levels, and were more tolerant to drought treatment, associated with improved RWC maintenance, higher proline accumulation, and reduced stomatal aperture under drought stress. Transcriptome analysis showed that genes involved in the processes like cell cycle, transcription and signal transduction, were substantially up-regulated in GaTOP6B-overexpressing Arabidopsis, promoting plant growth and development. More specifically, under drought stress, the genes involved in the biosynthesis of secondary metabolites such as phenylpropanoid, starch and sucrose were selectively enhanced to improve tolerance in plants. Taken together, the results demonstrated that GaTOP6B could coordinately regulate plant leaf and root growth via cellular endoreduplication, and positively respond to drought stress. Thus, GaTOP6B could be a competent candidate gene for improvement of drought tolerance in crop species.

Topics: Arabidopsis; Archaeal Proteins; Chlorophyll; Dehydration; DNA Topoisomerases, Type II; Endoreduplication; Flow Cytometry; Genes, Plant; Gossypium; Malondialdehyde; Plant Leaves; Plant Proteins; Plant Roots; Plant Transpiration; Plants, Genetically Modified; Proline; Real-Time Polymerase Chain Reaction

Symplastic interconnections of plant cells via perforations in adjoining cell walls (plasmodesmata) enable long-distance transport of photoassimilates and signaling substances required for growth and development. The pathways and features of intercellular movement of assimilates are often examined with fluorescent tracers whose molecular dimensions are similar to natural metabolites produced in photosynthesis. Chlorophyll fluorescence was recently found to be a sensitive noninvasive indicator of long-distance intracellular transport of physiologically produced photometabolites in characean internodes. The present work shows that the chlorophyll microfluorometry has a potential for studying the cell-to-cell transport of reducing substances released by local illumination of one internode and detected as the fluorescence increase in the neighbor internode. The method provides temporal resolution in the time frame of seconds and can be used to evaluate permeability of plasmodesmata to natural components released by illuminated chloroplasts. The results show that approximately one third of the amount of photometabolites released into the streaming cytoplasm during a 30-s pulse of local light permeates across the nodal complex with the characteristic time of ~ 10 s. The intercellular transport was highly sensitive to moderate elevations of osmolarity in the bath solution (150 mM sorbitol), which contrasts to the view that only transnodal gradients in osmolarity (and internal hydrostatic pressure) have an appreciable influence on plasmodesmal conductance. The inhibition of cell-to-cell transport was reversible and specific; the sorbitol addition had no influence on photosynthetic electron transport and the velocity of cytoplasmic streaming. The conductance of transcellular pores increased in the presence of the actin inhibitor cytochalasin D but the cell-to-cell transport was eventually suppressed due to the deceleration and cessation of cytoplasmic streaming. The results show that the permeability of plasmodesmata to low-molecular photometabolites is subject to upregulation and downregulation.

Topics: Cations, Divalent; Chara; Chlorophyll; Cytochalasin D; Cytophotometry; Cytoplasmic Streaming; Dehydration; Fluorescence; Hydrogen-Ion Concentration; Ionophores; Metabolome; Osmosis; Photosynthesis; Protons

We vapor print conformal conjugated polymer electrodes directly onto living plants and use these electrodes to probe the health of actively growing specimens using bioimpedance spectroscopy. Vapor-printed polymer electrodes, unlike their adhesive thin-film counterparts, do not delaminate from microtextured living surfaces as the organism matures and do not observably attenuate the natural growth pattern and self-sustenance of the plants investigated here. On-demand, noninvasive bioimpedance spectroscopy performed with long-lasting vapor-printed polymer electrodes can reliably detect deep tissue damage caused by dehydration and ultraviolet A exposure throughout the life cycle of a plant.

Topics: Chlorophyll; Dehydration; Ecological Parameter Monitoring; Electric Impedance; Electrodes; Flowers; Models, Biological; Plant Leaves; Plants; Polymers; Surface Properties; Ultraviolet Rays; Volatilization; Water

Previous studies have elucidated the mechanisms, ecological implications and constraints on transportation or sharing of defence signals among interconnected ramets of clonal plants suffering from localised herbivore damage. To our knowledge, few studies have been conducted to provide insights into the ecological implications on transportation or sharing of stress signals for clonal plants subjected to water stress. As a chemical elicitor, ABA can induce resistance response in plants suffering from water stress. A pot experiment was conducted to explore transportation or sharing of stress signals among interconnected ramets by using clonal fragments of Centella asiaticas (L.) Urban with four successive ramets (oldest, old, mature and young) subjected to low water availability (20% soil moisture contents). Compared with control, foliar oxidative stress of the old, mature and young ramets significantly decreased, and antioxidant capacity was increased when exogenous ABA was applied to the oldest ramets. Meanwhile, foliar PSII activity and chlorophyll content of the old, mature and young ramets significantly increased. Compared with control, biomass accumulation and ratio of below-ground/aboveground biomass of whole clonal fragments were significantly increased by ABA application to the oldest ramets. However, similar patterns were not observed when exogenous ABA was applied to the young ramets. Our results show that transportation or sharing of stress signals among interconnected ramets improves systemic resistance of clonal networks to water stress, which is dependent on directionality of vascular flows. Compared with the old or mature ramets, the young ramets displayed stronger resistance response (such as higher antioxidant enzymes activities and proline content, lower O2•- production rate and malondialdehyde content) to water stress as well as higher PSII activity and chlorophyll content when exogenous ABA was applied to the oldest ramets. Thus, transportation or sharing of stress signals may favour young ramets that are most valuable for growth and fitness of clonal plant subjected to environmental stress. It is suggested that transportation or sharing of stress signals among interconnected ramets may confer clonal plants with considerable benefits in adapting to spatio-temporal heterogeneous habitats.

Topics: Biomass; Chlorophyll; Dehydration; Ecosystem; Humans; Soil

Some mosses are extremely tolerant of drought stress. Their high drought tolerance relies on their ability to effectively dissipate absorbed light energy to heat under dry conditions. The energy dissipation mechanism in a drought-tolerant moss, Bryum argenteum, has been investigated using low-temperature picosecond time-resolved fluorescence spectroscopy. The results are compared between moss thalli samples harvested in Antarctica and in Japan. Both samples show almost the same quenching properties, suggesting an identical drought tolerance mechanism for the same species with two completely different habitats. A global target analysis was applied to a large set of data on the fluorescence-quenching dynamics for the 430-nm (chlorophyll-a selective) and 460-nm (chlorophyll-b and carotenoid selective) excitations in the temperature region from 5 to 77 K. This analysis strongly suggested that the quencher is formed in the major peripheral antenna of photosystem II, whose emission spectrum is significantly broadened and red-shifted in its quenched form. Two emission components at around 717 and 725 nm were assigned to photosystem I (PS I). The former component at around 717 nm is mildly quenched and probably bound to the PS I core complex, while the latter at around 725 nm is probably bound to the light-harvesting complex. The dehydration treatment caused a blue shift of the PS I emission peak via reduction of the exciton energy flow to the pigment responsible for the 725 nm band.

Topics: Antarctic Regions; Bryopsida; Chlorophyll; Dehydration; Droughts; Energy Transfer; Japan; Light; Models, Biological; Photosystem I Protein Complex; Photosystem II Protein Complex; Spectrometry, Fluorescence; Temperature

Peanut is an important edible oil crop plant whose quality and yield are greatly affected by drought. The process and molecular mechanisms of recovery from drought are also critical to its productivity, but are currently poorly characterized. Here, we investigate the involvement of peanut AhGLK1 in recovery from drought, and in particular its relationship with AhPORA, which encodes a key enzyme in chlorophyll biosynthesis. We found that chlorophyll content, chlorophyll fluorescence, AhPORA protein level and genes related to chlorophyll biosynthesis and photosynthesis declined markedly under drought conditions, but all increased during recovery. Consistent with this, AhGLK1 expression decreased during water stress and increased when the stress was removed. When AhGLK1 was transformed into Arabidopsis glk1glk2 mutant, it increased the survival rate of the mutant during recovery from drought and fully rescued the mutant's pale-green phenotype. In addition, chlorophyll content and fluorescence, and the expression of genes related to chlorophyll biosynthesis and photosynthesis, were all increased. Bioinformatics analysis and experimental evidence suggested that AhGLK1 augments the expression of AhPORA by binding to its promoter. Our findings confirm that AhGLK1 plays a role as a transcription factor that upregulates expression of AhPORA during post-drought recovery, thereby stimulating chlorophyll biosynthesis and photosynthesis.

Topics: Amino Acid Sequence; Arachis; Base Sequence; Chlorophyll; Dehydration; Droughts; Gene Expression Regulation, Plant; Photosynthesis; Plant Leaves; Plant Proteins; Transcription Factors

Ascorbate-glutathione (ASA-GSH) cycle is a major pathway of H2O2 scavenging and an effective mechanism of detoxification in plants. The differences in photosynthesis, chlorophyll content (Chl), relative water content (RWC), antioxidants and antioxidative enzyme activities involved in ASA-GSH metabolism were measured between the flag leaves and spike bracts (glumes and lemmas) during grain filling under drought stress. The expression of APX1, GRC1, DHAR, MDHAR, GPX1, and GS3 in ASA-GSH cycle was also measured. Compared with the flag leaves, the spike bracts exhibited stable net photosynthetic rate (PN) and chlorophyll content (Chl), a lower accumulation of reactive oxygen species (ROS), and more enhanced percentages of antioxidant enzyme activities and key enzymes gene transcription levels involved in ASA-GSH metabolism during the grain-filling stage under drought conditions. This could be the reasonable explanation for the more stable photosynthetic capacity in spikes, and the glumes and lemmas senesced later than the flag leaves at the late grain-filling stage. Also, the function of ASA-GSH cycle could not be ignored in alleviating oxidative damage by scavenging more excess ROS in spikes under drought stress.

Topics: Ascorbic Acid; Carbohydrate Metabolism; Chlorophyll; Dehydration; Droughts; Glutathione; Oxidative Stress; Photosynthesis; Plant Leaves; Seasons; Seedlings; Triticum

NAC transcription factors (TFs) are important regulators of plant adaptation to abiotic stress. In this study, we functionally characterized an NAC TF, ShNAC1, from Solanum habrochaites. ShNAC1 was up-regulated by drought, cold, and salt stresses, and it displayed lower expression at the late stage of stress treatments than its orthologous gene in S. lycopersicum. Overexpression of ShNAC1 in tomato resulted in reduced cold, drought, and salt tolerance. Additionally, ShNAC1 displayed the highest expression in senescent leaf, and overexpressing ShNAC1 accelerated salt- and dark-induced leaf senescence. ShNAC1 was located in the nucleus without transactivation activity. RNA-seq analysis revealed that 81% (190 out of 234) differentially-expressed genes (DEGs) showed down-regulation in the transgenic line L2 compared with wild-type, suggesting that ShNAC1 may function as a transcriptional repressor. Among these down-regulated DEGs, many were involved in stress responses, such as SlHKT1;1, SlMAPKKK59, SlJA2, SlTIL, SlALDH2B1, etc. Noticeably, one ACS gene and three ACO genes involved in ethylene biosynthesis were up-regulated, while seven ERF genes in the ethylene signal transduction pathway were down-regulated in the transgenic lines, respectively. Our results suggested that ShNAC1 negatively regulates tolerance to abiotic stress in tomato probably by modulating the ethylene biosynthesis and signal transduction pathways.

Topics: Chlorophyll; Cold-Shock Response; Dehydration; Gene Expression Regulation, Plant; Genes, Plant; Plant Proteins; Salt Tolerance; Solanum; Trans-Activators

The impact of water-stress on chloroplast development was studied by applying polyethylene glycol 6000 to the roots of 5-day-old etiolated rice (Oryza sativa) seedlings that were subsequently illuminated up to 72 h. Chloroplast development in drought environment led to down-regulation of light-harvesting Chl-proteins. Photosynthetic proteins of Photosystem II (PSII) and oxygen evolving complex i.e., Cytb559, OEC16, OEC23 and OEC33 as well as those of PSI such as PSI-III, PSI-V, and PSI-VI, decreased in abundance. Consequently, due to reduced light absorption by antennae, the electron transport rates of PSII and PSI decreased by 55% and 25% respectively. Further, seedling development in stress condition led to a decline in the ratio of variable (Fv) to maximum (Fm) Chl a fluorescence, as well in the quantum yield of PSII photochemistry. Addition of Mg

Topics: Antioxidants; Chlorophyll; Chloroplasts; Dehydration; Droughts; Electron Transport; Fluorescence; Light; Light-Harvesting Protein Complexes; Oryza; Oxidation-Reduction; Oxidative Stress; Photosynthesis; Photosystem I Protein Complex; Photosystem II Protein Complex; Plant Roots; Seedlings; Thylakoids

Basic region/leucine zipper (bZIP) transcription factors perform as crucial regulators in ABA-mediated stress response in plants. Nevertheless, the functions for most bZIP family members in tomato remain to be deciphered.. Here we examined the functional characterization of SlbZIP1 under salt and drought stresses in tomato. Silencing of SlbZIP1 in tomato resulted in reduced expression of multiple ABA biosynthesis- and signal transduction-related genes in transgenic plants. In stress assays, SlbZIP1-RNAi transgenic plants exhibited reduced tolerance to salt and drought stresses compared with WT plants, as are evaluated by multiple physiological parameters associated with stress responses, such as decreased ABA, chlorophyll contents and CAT activity, and increased MDA content. In addition, RNA-seq analysis of transgenic plants revealed that the transcription levels of multiple genes encoding defense proteins related to responses to abiotic stress (e.g. endochitinase, peroxidases, and lipid transfer proteins) and biotic stress (e.g. pathogenesis-related proteins) were downregulated in SlbZIP1-RNAi plants, suggesting that SlbZIP1 plays a role in regulating the genes related to biotic and abiotic stress response.. Collectively, the data suggest that SlbZIP1 exerts an essential role in salt and drought stress tolerance through modulating an ABA-mediated pathway, and SlbZIP1 may hold potential applications in the engineering of salt- and drought-tolerant tomato cultivars.

Topics: Abscisic Acid; Basic-Leucine Zipper Transcription Factors; Catalase; Chlorophyll; Dehydration; Gene Expression Regulation, Plant; Gene Silencing; Genes, Plant; Malondialdehyde; Plant Proteins; Plants, Genetically Modified; Salt Stress; Solanum lycopersicum

The plant cuticle, composed of cutin and waxes, is a hydrophobic layer coating the aerial organs of terrestrial plants and playing a critical role in limiting water loss. While melatonin has been recently demonstrated to be involved in responses to drought stress in plants, its relationship with cuticle formation is not known. In the present work, we report the effects of melatonin on the formation of cuticle in tomato leaves subjected to water deficit. Preliminary analysis by light microscope showed that tomato leaves pretreated with exogenous melatonin might have thicker cutin than tomato leaves without melatonin pretreatment under water deficit condition. Chemical characterization showed that exogenous application of melatonin increased the level of cuticular waxes in tomato leaves under water deficit. Consistent with the change in cuticular waxes was the increased abundance of wax-associated gene transcripts. Further, assessment of water loss and chlorophyll leaching in tomato leaves revealed the association of cuticle deposition with reduced leaf permeability, which is important in restricting water loss in water deficit-stressed tomato plants. These results suggest a role for melatonin in regulating leaf cuticle formation and non-stomatal water loss in leaves.

Topics: Chlorophyll; Dehydration; Droughts; Gene Expression Regulation, Plant; Melatonin; Membrane Lipids; Permeability; Plant Leaves; Plant Proteins; Solanum lycopersicum; Water; Waxes

Grapes are one of the most important fruits because of their economic and nutritional benefits, and grapevines are widely cultivated in arid and semi-arid areas. Therefore, it is critical to study the mechanism by which grapevines respond to water stress. In this research, micro-morphological and metabolomic analyses were conducted to evaluate the effects of water stress on stomatal morphology and volatile compounds extracted from the leaves of grapevine plants. There were two treatments: well-watered plants (watered daily) and drought-stressed plants (no irrigation). Plant weights were recorded, and the well-watered plants were irrigated daily to replace the water lost to evapotranspiration. The water status of the grapevines was determined according to their relative water content. The changes in proline content, hydrogen peroxide content, lipid peroxidation and antioxidant activities, as well as those of photosynthetic parameters and chlorophyll fluorescence, were monitored as markers of water stress. The microscopic changes in stomatal behavior were observed using a scanning electron microscope. A total of 12 secondary volatile compounds, including aldehydes, ketones and alcohols, were detected in the grapevine leaves. Among them, (E)-2-hexenal and 3-hexenal showed a significant increase after water stress. Multivariate statistical analysis revealed that the levels of 3-hexenal and (E)-2-hexenal were closely related to the changes in proline, hydrogen peroxide (H

Topics: Aldehydes; Chlorophyll; Dehydration; Fatty Acids, Volatile; Metabolomics; Photosynthesis; Plant Leaves; Plant Stomata; Vitis

Mediterranean tomato landraces adapted to arid environments represent an option to counteract drought, and to address the complexity of responses to water deficit and recovery, which is a crucial component of plant adaptation mechanisms. We investigated physiological, biochemical and molecular responses of two Mediterranean tomato landraces, 'Locale di Salina' (Lc) and 'Pizzutello di Sciacca' (Pz) under two dehydration periods and intermediate rehydration in greenhouse pot experiments. Relationship between CO

Topics: Abscisic Acid; Carbon Dioxide; Chlorophyll; Chlorophyll A; Dehydration; Fluorescence; Mediterranean Region; Photosynthesis; Plant Growth Regulators; Plant Leaves; Plant Stomata; Poly(ADP-ribose) Polymerases; Polymerase Chain Reaction; Proline; Solanum lycopersicum

In the Mediterranean region, grapevines usually deal with drought during their summer growth season. Concurrently, grapevines are hosts to a large number of viruses from which grapevine leafroll associated virus-3 is one of the most widespread and provokes considerable economic losses in many vineyards. However, information concerning grapevine metabolic responses to the combination of drought and viral infection is scarce. Gas-chromatography coupled to mass-spectrometry based metabolite profiling was used in combination with growth analysis, viral loads and gas exchange data to perform an integrative study of the effects of individual and combined stress in two Majorcan grapevine varieties at two experimental years. Metabolic responses of both varieties to the combination of water stress and virus infection were specific and not predicted from the sum of single stress responses. Correlations between respiration, biomass and key metabolites highlight specific adjustments of respiratory and amino acid metabolism possibly underlying the maintenance of carbon balance and growth in grapevines under stress combination.

Topics: Cell Respiration; Chlorophyll; Closteroviridae; Dehydration; Plant Diseases; Plant Leaves; Vitis

In order to understand the effects of sugar metabolism on drought resistance in wheat, two wheat cultivars with different levels of drought resistance were used in this study. We investigated the accumulation pattern of water-soluble carbohydrates (WSC) and expression profiles of twelve fructan metabolism-related genes in peduncle (PED), penultimate (PEN), and lower internode (LOW) stem tissues under drought stress. LH7, a higher drought-resistance cultivar, contained a higher stem dry weight and higher content of WSC in PED, PEN, and LOW tissues, while XN979, a lower drought-resistance cultivar, contained lower values. The tissues from LOW internodes had the highest WSC content, while PED had the lowest. The mRNA levels of genes encoding fructan synthesis-related enzymes, sucrose: sucrose 1-fructosyltransferase (1-SST), sucrose: fructan 6-fructosyltransferase (6-SFT), and fructan: fructan 1- fructosyltransferase (1-FFT) showed higher expression levels at early time points following stress, whilst the genes encoding degradation-related enzymes, fructan exohydrolases (1-FEH), and invertase (INV), showed higher expression at a later time point. Compared with XN979, LH7 showed higher expression levels of genes encoding fructan synthesis-related enzymes at all growth stages, whilst the expression of 1-FEH-W3, 6-FEH, and INV3 were higher at a later stage; these expression levels would benefit fructan accumulation and remobilization at early and later stages, respectively. Drought stress induced most of fructan metabolism related genes expression level decreasing in LH7 PED, but enhancing in LH7 LOW part at early time points following stress. The results confirm that there are complex, coordinated expression patterns of fructan synthesis- and degradation-related genes in stems under drought stress. In summary, 1-SST-A2, 6-SFT, 1-FFT-A, 1-FEH-W3, 6-FEH, and INV3 play important roles in fructan accumulation. In addition, higher expression of genes related to fructan synthesis and degradation occurs during early and later stages of drought stress, respectively, enhancing the drought resistance of wheat cultivar LH7.

Topics: Carbohydrate Metabolism; Chlorophyll; Dehydration; Gene Expression Regulation, Plant; Metabolic Networks and Pathways; Plant Leaves; Plant Stems; Real-Time Polymerase Chain Reaction; Triticum

Genetic improvement is an important approach for crop improvement towards yield stability in stress-prone areas. Functional analysis of candidate stress response genes can provide key information to allow the selection and modification of improved crop varieties. In this study, the constitutive expression of a banana cDNA, MaRHD3 in Arabidopsis improved the ability of transgenic lines to adapt to drought conditions. Transgenic Arabidopsis plants expressing MaRHD3 had roots with enhanced branching and more root hairs when challenged with drought stress. The MaRHD3 plants had higher biomass accumulation, higher relative water content, higher chlorophyll content and an increase in activity of reactive oxygen species (ROS) scavenging enzymes; SOD, CAT, GR, POD and APX with reduced water loss rates compared to control plants. The analysis of oxidative damage indicated lower cell membrane damage in transgenic lines compared to control plants. These findings, together with data from higher expression of ABF-3 and higher ABA content of drought-stressed transgenic MaRHD3 expressing plants, support the involvement of the ABA signal pathway and ROS scavenging enzyme systems in MaRHD3 mediated drought tolerance.

Topics: Abscisic Acid; Arabidopsis; Chlorophyll; Dehydration; Musa; Plant Growth Regulators; Plant Proteins; Plant Roots; Plants, Genetically Modified; Reactive Oxygen Species; Real-Time Polymerase Chain Reaction

Bioactive compounds, vitamins, phenolic acids, flavonoids of A. tricolor are the sources of natural antioxidant that had a great importance for the food industry as these detoxify ROS in the human body. These natural antioxidants protect human from many diseases such as cancer, arthritis, emphysema, retinopathy, neuro-degenerative cardiovascular diseases, atherosclerosis and cataracts. Moreover, previous literature has shown that drought stress elevated bioactive compounds, vitamins, phenolics, flavonoids and antioxidant activity in many leafy vegetables. Hence, we study the nutritional and bioactive compounds, phenolic acids, flavonoids and antioxidant capacity of amaranth under drought stress for evaluation of the significant contribution of these compounds in the human diet.. The genotype VA3 was assessed at four drought stress levels that significantly affected nutritional and bioactive compounds, phenolic acids, flavonoids and antioxidant capacity. Protein, ash, energy, dietary fiber, Ca, K, Cu, S, Mg, Mn, Mo, Na, B content, total carotenoids, TFC, vitamin C, TPC, TAC (DPPH), betacarotene, TAC (ABTS. In A. tricolor, drought stress enhanced the quantitative and qualitative improvement of nutritional and bioactive compounds, phenolic acids, flavonoids and antioxidants. Hence, farmers of semi-arid and dry areas of the world could be able to grow amaranth as a substitute crop.

Topics: Amaranthus; Antioxidants; Ascorbic Acid; Bangladesh; Carotenoids; Chlorophyll; Dehydration; Droughts; Flavonoids; Genotype; Hydroxybenzoates; Minerals; Pigments, Biological; Plant Leaves; Polyphenols

The fraction of absorbed photosynthetically active radiation (FPAR) is a key variable in the model of vegetation productivity. Vegetation indices (VIs) that were derived from instantaneous remote-sensing data have been successfully used to estimate the FPAR of a day or a longer period. However, it has not yet been verified whether continuous VIs can be used to accurately estimate the diurnal dynamics of a vegetation canopy FPAR, which may fluctuate dramatically within a day. In this study, we measured the high temporal resolution spectral data (480 to 850 nm) and FPAR data of a maize canopy from the jointing stage to the tasseling stage under different irrigation and illumination conditions using two automatic observation systems. To estimate the FPAR, we developed regression models based on a quadratic function using 13 kinds of VIs. The results show the following: (1) Under nondrought conditions, although the illumination condition (sunny or cloudy) influenced the trend of the canopy diurnal FPAR, it had only a slight effect on the model accuracies of the FPAR-VIs. The maximum coefficients of determination (R²) of the FPAR-VIs models generated for the sunny nondrought data, the cloudy nondrought data, and all of the nondrought data were 0.895, 0.88, and 0.828, respectively. The VIs-including normalized difference vegetation index (NDVI), green NDVI (GNDVI), red-edge simple ratio (SR

Topics: Chlorophyll; Dehydration; Light; Photosynthesis; Plant Leaves; Soil; Zea mays

Water stress is one of the important abiotic environmental stresses that threaten the agricultural -products in the world. This experiment was carried out to determine the effect of water stress on physiological and biochemical characteristics of three commercial olive cultivars. A factorial pot experiment was conducted in the field conditions using completely randomized design in Gilan-Gharb city, Kermanshah province during 2015. One-year-old rooted cuttings of Zard, Amigdalolia and Konservolia olive cultivars were planted in 12-liter pots and subjected to three irrigation treatments. Irrigation treatments included control (100% of field capacity), 75% and 50% field capacity. Physiological and biochemical characteristics such as relative water content (RWC), electrolyte leakage (EL), calcium, potassium and sodium content, total phenol, malondialdehyde, peroxidases, catalase, a, b and total chlorophyll, proline and total carbohydrate were measured. Results showed that relative water content, K & Ca was reduced while sodium content increased by increasing water stress. Chlorophyll content was higher in Konservolia cultivar under water stress in comparison to the others. Water stress induced increasing in proline, total phenol and soluble carbohydrate in all cultivars. The highest total phenol and proline was recorded in Zard cultivar under water stress. Total carbohydrate increased significantly (P<0.05) in Konservolia in comparison to the others. Malondialdehyde content was increased as an index of oxidative stress by drought. The highest peroxidases and catalase activity were recorded under drought stress of 50% irrigation in Konservolia olive cultivar. Generally based on results Konservolia, Zard and Amigdalolia were more tolerant respectively.

Topics: Carbohydrates; Catalase; Chlorophyll; Dehydration; Electrolytes; Malondialdehyde; Olea; Peroxidases; Plant Leaves; Proline; Solubility; Stress, Physiological; Water

Drought stress during grain filling is the most yield-damaging to wheat. Pre-drought priming facilitated the wheat plants to sustain grain development against the post-anthesis drought stress by modulating the levels of growth hormones. Post-anthesis drought stress substantially reduces grain yield in wheat (Triticum aestivum L.) due to impaired grain development associated with imbalanced levels of growth hormones. To investigate whether pre-drought priming could sustain grain development in wheat by regulating favorable levels of growth hormones under post-anthesis drought conditions, the plants of a drought-sensitive (Yangmai-16) and drought-tolerant (Luhan-7) wheat cultivar were exposed to a moderate drought stress during tillering (Feekes 2 stage) for priming, and then, a subsequent severe drought stress was applied from 7 to 14 days after anthesis. The results showed that drought-stressed plants of both cultivars showed a decline in flag leaf water potential, chlorophyll contents, photosynthetic rate, grain size initiation, and grain filling as compared to well-watered plants; however, decline in these traits was less in pre-drought primed (PD) plants than in nonprimed (ND) plants. Under drought stress, the PD plants regulated higher concentrations of zeatin and zeatin riboside, indole-3-acetic acid, gibberellins, and lower abscisic acid content in grains, resulting in higher endosperm cell division and expansion, grain size initiation, grain-filling rate and duration, and finally higher grain dry weights as compared to ND plants. The PD plants of both cultivars showed higher potential to tolerate the post-anthesis drought stress, but more effect was displayed by drought-tolerant cultivar. From the achieved results, it was concluded that pre-drought priming facilitated the wheat plants to sustain higher grain development and yield against the most yield-damaging post-anthesis drought stress by modulating the levels of growth hormones.

Topics: Cell Division; Chlorophyll; Dehydration; Droughts; Endosperm; Photosynthesis; Plant Growth Regulators; Plant Leaves; Seeds; Triticum; Water

Tropospheric ozone (O

Topics: Biomass; China; Chlorophyll; Dehydration; Droughts; Light; Ozone; Photosynthesis; Plant Leaves; Populus; Stress, Physiological; Water

Boea hygrometrica (B. hygrometrica) can tolerate severe desiccation and resume photosynthetic activity rapidly upon water availability. However, little is known about the mechanisms by which B. hygrometrica adapts to dehydration and resumes competence upon rehydration. Here we determine how B. hygrometrica deals with oxidative stress, excessive excitation/electron pressures as well as photosynthetic apparatus modulation during dehydration/rehydration. By measuring ROS generation and scavenging efficiency, we found that B. hygrometrica possesses efficient strategies to maintain cellular redox homeostasis. Transmission electron microscopy (TEM) analysis revealed a remarkable alteration of chloroplast architecture and plastoglobules (PGs) accumulation during dehydration/rehydration. Pulse-amplitude modulated (PAM) chlorophyll fluorescence measurements, P700 redox assay as well as chlorophyll fluorescence emission spectra analysis on leaves of B. hygrometrica during dehydration/rehydration were also performed. Results showed that the photochemical activity of PSII as well as photoprotective energy dissipation in PSII undergo gradual inactivation/activation during dehydration/rehydration in B. hygrometrica; PSI activity is relatively induced upon water deficit, and dehydration leads to physical interaction between PSI and LHCII. Furthermore, blue-native polyacrylamide gel electrophoresis (BN-PAGE) and immunoblot analysis revealed that the protein abundance of light harvesting complexes decrease markedly along with internal water deficit to restrict light absorption and attenuate electron transfer, resulting in limited light excitation and repressed photosynthesis. In contrast, many thylakoid proteins remain at a basal level even after full dehydration. Taken together, our study demonstrated that efficient modulation of cellular redox homeostasis and photosynthetic activity confers desiccation tolerance in B. hygrometrica.

Topics: Antioxidants; Chlorophyll; Chloroplasts; Dehydration; Energy Metabolism; Homeostasis; Light-Harvesting Protein Complexes; Magnoliopsida; Photosynthesis; Photosystem II Protein Complex; Plant Cells; Thylakoids; Water

Despite the importance of growth [CO 2 ] and water availability for tree growth and survival, little information is available on how the interplay of these two factors can shape intraspecific patterns of functional variation in tree species, particularly for conifers. The main objective of the study was to test whether the range of realized drought tolerance within the species can be affected by elevated [CO 2 ].. Intraspecific variability in leaf gas exchange, growth rate and other leaf functional traits were studied in clones of maritime pine. A factorial experiment including water availability, growth [CO 2 ] and four different genotypes was conducted in growth rooms. A 'water deficit' treatment was imposed by applying a cycle of progressive soil water depletion and recovery at two levels of growth [CO 2 ]: 'ambient [CO 2 ]' (aCO 2 400 μmol mol -1 ) and 'elevated [CO 2 ]' (eCO 2 800 μmol mol -1 ).. eCO2 had a neutral effect on the impact of drought on growth and leaf gas exchange of the most drought-sensitive genotypes while it aggravated the impact of drought on the most drought-tolerant genotypes at aCO2. Thus, eCO2 attenuated genotypic differences in drought tolerance as compared with those observed at aCO2. Genotypic variation at both levels of growth [CO2] was found in specific leaf area and leaf nitrogen content but not in other physiological leaf traits such as intrinsic water use efficiency and leaf osmotic potential. eCO2 increased Δ 13 C but had no significant effect on δ 18 O. This effect did not interact with the impact of drought, which increased δ 18 O and decreased Δ 13 C. Nevertheless, correlations between Δ 13 C and δ 18 O indicated the non-stomatal component of water use efficiency in this species can be particularly sensitive to drought.. Evidence from this study suggests elevated [CO 2 ] can modify current ranges of drought tolerance within tree species.

Topics: Carbon Dioxide; Chlorophyll; Dehydration; Genotype; Nitrogen; Osmotic Pressure; Photosynthesis; Pinus; Plant Leaves

Drought stress is one of the major natural challenges in the main tea-producing regions of China. The tea plant (Camellia sinensis) is a traditional beverage plant whose growth status directly affects tea quality. Recent studies have revealed that microRNAs (miRNAs) play key functions in plant growth and development. Although some miRNAs have been identified in C. sinensis, little is known about their roles in the drought stress response of tea plants.. Physiological characterization of Camellia sinensis 'Tieguanyin' under drought stress showed that the malondialdehyde concentration and electrical conductivity of leaves of drought-stressed plants increased when the chlorophyll concentration decreased under severe drought stress. We sequenced four small-RNA (sRNA) libraries constructed from leaves of plants subjected to four different treatments, normal water supply (CK); mild drought stress (T1); moderate drought stress (T2) and severe drought stress (T3). A total of 299 known mature miRNA sequences and 46 novel miRNAs were identified. Gene Ontology enrichment analysis revealed that most of the differentially expressed-miRNA target genes were related to regulation of transcription. Kyoto Encyclopedia of Genes and Genomes analysis revealed that the most highly enriched pathways under drought stress were D-alanine metabolism, sulfur metabolism, and mineral absorption pathways. Real-time quantitative PCR (qPCR) was used to validate the expression patterns of 21 miRNAs (2 up-regulated and 19 down-regulated under drought stress). The observed co-regulation of the miR166 family and their targets ATHB-14-like and ATHB-15-like indicate the presence of negative feedback regulation in miRNA pathways.. Analyses of drought-responsive miRNAs in tea plants showed that most of differentially expressed-miRNA target genes were related to regulation of transcription. The results of study revealed that the expressions of phase-specific miRNAs vary with morphological, physiological, and biochemical changes. These findings will be useful for research on drought resistance and provide insights into the mechanisms of drought adaptation and resistance in C. sinensis.

Topics: Camellia sinensis; Chlorophyll; Dehydration; Gene Expression Regulation, Plant; Gene Ontology; Genes, Plant; MicroRNAs; Real-Time Polymerase Chain Reaction; Sequence Analysis, DNA

Current practice in neurosurgical needle insertion is limited by the straight trajectories inherent with rigid probes. One technique allowing curvilinear trajectories involves flexible bevel-tipped needles, which bend during insertion due to their asymmetry. In the brain, safety will require avoidance of the sharp tips often used in laboratory studies, in favor of a more rounded profile. Steering performance, on the other hand, requires maximal asymmetry. Design of safe bevel-tipped brain needles thus involves management of this tradeoff by adjusting needle gauge, bevel angle, and fillet (or tip) radius to arrive at a design that is suitably asymmetrical while producing strain, strain rate, and stress below the levels that would damage brain tissue.. The prototype needle selected was 1.66 mm in diameter, with bevel angle of 10° and fillet radius of 0.25 mm. Upon examination of postoperative CT and histological images, no differences in tissue trauma or hemorrhage were noted between the prototype needle and the Sedan needle.. The study indicates a general design technique for safe bevel-tipped brain needles based on comparison with relevant damage thresholds for strain, strain rate, and stress. The full potential of the technique awaits the determination of more exact safety thresholds.. Immunological responses after stings varied in bee and vespid venom-allergic patients. In patients under VIT, sIgE and sIgG

Topics: Adolescent; Adult; Aged; Aged, 80 and over; Alkaline Phosphatase; Allergens; Animals; Antibody Specificity; Arthropod Venoms; Asymptomatic Diseases; Biological Variation, Population; Blood Cells; Catalysis; CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; Cell Count; Chlorophyll; Chlorophyll A; Chlorophyta; Cross-Sectional Studies; Crystallography, X-Ray; Dehydration; Dietary Supplements; Electromagnetic Fields; Enzyme-Linked Immunosorbent Assay; Erythrocytes; Exercise; Exercise Therapy; Female; Goats; Hand Strength; Hemoglobins; Humans; Hypersensitivity; Immunoassay; Immunoglobulin E; Immunoglobulin G; Insect Bites and Stings; Lab-On-A-Chip Devices; Lichens; Light; Long-Term Care; Male; Mice; Microfluidic Analytical Techniques; Middle Aged; Muscle, Skeletal; Mutation; Osmotic Pressure; Paper; Peroxidases; Phenotype; Photosynthetic Reaction Center Complex Proteins; Polychaeta; Powders; Rabbits; Radio Frequency Identification Device; Rats; Rats, Sprague-Dawley; Sarcopenia; Seasons; Sensitivity and Specificity; Spectrophotometry, Ultraviolet; Streptavidin; Substrate Specificity; Textiles; Water; Whole-Body Irradiation; Young Adult

Topics: Ascorbate Peroxidases; Ascorbic Acid; Carotenoids; Catalase; Chlorophyll; Dehydration; Free Radical Scavengers; Glutathione Reductase; Lipid Peroxidation; Monophenol Monooxygenase; Osmoregulation; Osmotic Pressure; Oxidative Stress; Phenylalanine Ammonia-Lyase; Plant Proteins; Potassium; Sodium; Superoxide Dismutase; Triticum

The green algal genus Klebsormidium (Klebsormidiophyceae, Streptophyta) is a typical member of biological soil crusts (BSCs) worldwide. Ecophysiological studies focused so far on individual strains and thus gave only limited insight on the plasticity of this genus. In the present study, 21 Klebsormidium strains (K. dissectum, K. flaccidum, K. nitens, K. subtile) from temperate BSCs in Central European grassland and forest sites were investigated. Photosynthetic performance under desiccation and temperature stress was measured under identical controlled conditions. Photosynthesis decreased during desiccation within 335-505 min. After controlled rehydration, most isolates recovered, but with large variances between single strains and species. However, all K. dissectum strains had high recovery rates (>69%). All 21 Klebsormidium isolates exhibited the capability to grow under a wide temperature range. Except one strain, all others grew at 8.5 °C and four strains were even able to grow at 6.2 °C. Twenty out of 21 Klebsormidium isolates revealed an optimum growth temperature >17 °C, indicating psychrotrophic features. Growth rates at optimal temperatures varied between strains from 0.26 to 0.77 μ day

Topics: Adaptation, Physiological; Biodiversity; Chlorophyll; Chlorophyll A; Dehydration; Desiccation; DNA, Ribosomal; Ecology; Ecosystem; Forests; Germany; Grassland; Photosynthesis; Phylogeny; Soil; Streptophyta; Stress, Physiological; Temperature

Desiccation-tolerant plants (Craterostigma plantagineum and Lindernia brevidens) evolved a highly efficient strategies to prevent dehydration-induced irreversible damage. The protection system involves synthesis of LEA proteins, decrease of photosynthetic activity and activation of antioxidant systems. The regulation of these processes requires joint action of multiple proteins. Here, we present comparative analyses of accumulation of transcripts encoding components of the protection machinery, such as selected LEA proteins, enzymes of the chlorophyll degradation pathway and anthocyanin biosynthesis enzymes in total and polysomal RNA pools. The analyses revealed that desiccation-tolerant plants recruit mRNAs to ribosomes with higher efficiency than the desiccation-sensitive species L. subracemosa. Desiccation-tolerant species accumulated high amounts of LEA transcripts during dehydration and precisely controlled the amounts of chlorophyll keeping it at a level sufficient to activate photosynthesis after rehydration. In contrast, mRNA of L. subracemosa was prone to dehydration-induced degradation, decomposition of the photosynthetic apparatus and degradation of free chlorophyll. Thus, the results of the studies point to differences in the control of gene expression and degradation of chlorophyll in desiccation-tolerant versus desiccation-sensitive species when the plants were subjected to dehydration.

Topics: Adaptation, Physiological; Anthocyanins; Chlorophyll; Craterostigma; Dehydration; Droughts; Gene Expression Regulation, Plant; Genetic Phenomena; Magnoliopsida; Photosynthesis; Pigments, Biological; Plant Proteins; RNA Stability; RNA, Messenger; RNA, Plant; Species Specificity; Stress, Physiological; Water

The submergence-tolerance regulator, SUBMERGENCE1A (SUB1A), of rice (Oryza sativa L.) modulates gene regulation, metabolism and elongation growth during submergence. Its benefits continue during desubmergence through protection from reactive oxygen species and dehydration, but there is limited understanding of SUB1A's role in physiological recovery from the stress. Here, we investigated the contribution of SUB1A to desubmergence recovery using the two near-isogenic lines, submergence-sensitive M202 and tolerant M202(Sub1). No visible damage was detected in the two genotypes after 3 d of submergence, but the sublethal stress differentially altered photosynthetic parameters and accumulation of energy reserves. Submergence inhibited photosystem II photochemistry and stimulated breakdown of protein and accumulation of several amino acids in both genotypes at similar levels. Upon desubmergence, however, more rapid return to homeostasis of these factors was observed in M202(Sub1). Submergence considerably restrained non-photochemical quenching (NPQ) in M202, whereas the value was unaltered in M202(Sub1) during the stress. Upon reaeration, submerged plants encounter sudden exposure to higher light. A greater capability for NPQ-mediated photoprotection can benefit the rapid recovery of photosynthetic performance and energy reserve metabolism in M202(Sub1). Our findings illuminate the significant role of SUB1A in active physiological recovery upon desubmergence, a component of enhanced tolerance to submergence.

Topics: Adaptation, Physiological; Amino Acids; Ammonium Compounds; Carbon; Chlorophyll; Dehydration; Fluorescence; Gene Expression Regulation, Plant; Nitrates; Nitrogen; Oryza; Oxidative Stress; Oxygen; Photosynthesis; Plant Leaves; Plant Proteins; Stress, Physiological

Senescence can be delayed in transgenic plants overexpressing the enzyme isopentenyltransferase (IPT) due to stress-induced increased levels of endogenous cytokinins. This trait leads to sustained photosynthetic activity and improved tolerance to abiotic stress. The aim of this study was to generate and characterize transgenic plants of maize (Zea mays L.) transformed with the IPT gene sequence under the regulation of SARK promoter (protein kinase receptor-associated senescence). Three independent transgenic events and their segregating null controls were evaluated in two watering regimes (WW: well watered; WD: water deficit) imposed for two weeks around anthesis. Our results show that the WD treatment induced IPT expression with the concomitant increase in cytokinin levels, which prolonged the persistence of total green leaf area, and maintained normal photosynthetic rate and stomatal conductance. These trends were accompanied by a minor decrease in number of grains per plant, individual grain weight and plant grain yield as compared to WW plants. Plants expressing the IPT gene under WD had PGR, anthesis and silking dates and biomass levels similar to WW plants. Our results demonstrate that expression of the IPT gene under the regulation of the SARK promoter helps improve productivity under WD conditions in C4 plants like maize.

Topics: Abscisic Acid; Alkyl and Aryl Transferases; Biomass; Blotting, Southern; Chlorophyll; Cyclopentanes; Cytokinins; Dehydration; Edible Grain; Oxylipins; Photosynthesis; Plant Proteins; Plant Stomata; Plants, Genetically Modified; Polymerase Chain Reaction; Promoter Regions, Genetic; Soil; Stress, Physiological; Water; Zea mays

Topics: Antioxidants; Chlorophyll; Chloroplasts; Dehydration; Photosynthesis; Thylakoids; Triticum

Aloe vera L. is one of the most important medicinal plants in the world. In order to determine the effects of light intensity and water deficit stress on chlorophyll (Chl) fluorescence and pigments of A. vera, a split-plot in time experiment was laid out in a randomized complete block design with four replications in a research greenhouse. The factorial combination of three light intensities (50, 75 and 100% of sunlight) and four irrigation regimes (irrigation after depleting 20, 40, 60 and 80% of soil water content) were considered as main factors. Sampling time was considered as sub factor. The first, second and third samplings were performed 90, 180 and 270 days after imposing the treatments, respectively. The results demonstrated that the highest light intensity and the severe water stress decreased maximum fluorescence (Fm), variable fluorescence (Fv)/Fm, quantum yield of PSII photochemistry (ФPSII), Chl and photochemical quenching (qP) but increased non-photochemical quenching (NPQ), minimum fluorescence (F0) and Anthocyanin (Anth). Additionally, the highest Fm, Fv/Fm, ФPSII and qP and the lowest NPQ and F0 were observed when 50% of sunlight was blocked and irrigation was done after 40% soil water depletion. Irradiance of full sunlight and water deficit stress let to the photoinhibition of photosynthesis, as indicated by a reduced quantum yield of PSII, ФPSII, and qP, as well as higher NPQ. Thus, chlorophyll florescence measurements provide valuable physiological data. Close to half of total solar radiation and irrigation after depleting 40% of soil water content were selected as the most efficient treatments.

Topics: Agricultural Irrigation; Aloe; Analysis of Variance; Anthocyanins; Chlorophyll; Dehydration; Fluorescence; Light; Photochemical Processes

Drought is an increasingly important limitation on plant productivity worldwide. Understanding the mechanisms of drought tolerance in plants can lead to new strategies for developing drought-tolerant crops. Many moss species are able to survive desiccation-a more severe state of dehydration than drought. Research into the mechanisms and evolution of desiccation tolerance in basal land plants is of particular significance to both biology and agriculture. In this study, we conducted morphological, cytological, and physiological analyses of gametophytes of the highly desiccation-tolerant bryophyte Atrichum undulatum (Hedw.) P. Beauv during dehydration and rehydration. Our results suggested that the mechanisms underlying the dehydration-recovery cycle in A. undulatum gametophytes include maintenance of membrane stability, cellular structure protection, prevention of reactive oxygen species (ROS) generation, elimination of ROS, protection against ROS-induced damage, and repair of ROS-induced damage. Our data also indicate that this dehydration-recovery cycle consists not only of the physical removal and addition of water, but also involves a highly organized series of cytological, physiological, and biochemical changes. These attributes are similar to those reported for other drought- and desiccation-tolerant plant species. Our findings provide major insights into the mechanisms of dehydration-tolerance in the moss A. undulatum.

Topics: Bryophyta; Chlorophyll; Dehydration; Droughts; Fluorescence; Phenotype; Photosynthesis

Adaptations allowing plants to cope with drying are particularly relevant in the light of predicted climate change. Dehydration tolerance (DhT, also dehydration-tolerant) is one such adaptation enabling tissue to survive substantial drying. A great deal of work has been conducted on highly DhT species. However, bryophytes showing less intense and variable DhT are understudied, despite the potential for these species to provide an informative link between highly tolerant and sensitive species. In this study, we tested the degree to which DhT varies across populations and the sexes of a species expected to exhibit a moderate DhT phenotype.. To test predicted patterns of tolerance we assessed DhT in males and females of Marchantia inflexa from two distinct habitat types that differ in water availability. Both common garden and field-collected tissue was subjected to drying assays at multiple intensities and recovery was monitored by chlorophyll florescence. Verification studies were conducted to confirm the level of dehydration, the rate of drying and the associated changes in photosynthetic physiology.. We confirmed our expectation that M. inflexa is able to tolerate moderate dehydration. We also found that females exhibited higher DhT than males, but populations did not differ in DhT when cultured in a common garden. However, field-collected samples exhibited differences in DhT corresponding to environmental dryness, suggesting plasticity in DhT.. By studying a less extreme DhT phenotype we gained insight into how more sensitive (yet still tolerant) organisms cope with dehydration. Additionally, the identified sex-specific variation in DhT may explain ecological patterns such as female-biased sex ratios. Furthermore, plasticity in DhT has the potential to inform management practices aimed at increasing tolerance to drought conditions.

Topics: Adaptation, Physiological; Chlorophyll; Climate Change; Dehydration; Droughts; Ecosystem; Hepatophyta; Light; Photosynthesis; Water

Physiological, biochemical and morpho-anatomical traits that determine the phenotypic plasticity of plants under drought were tested in two Arundinoideae with contrasting habitats, growth traits and metabolism: the fast-growing Arundo donax, which also is a strong isoprene emitter, and the slow-growing Hakonechloa macra that does not invest on isoprene biosynthesis. In control conditions, A. donax displayed not only higher photosynthesis but also higher concentration of carotenoids and lower phenylpropanoid content than H. macra. In drought-stressed plants, photosynthesis was similarly inhibited in both species, but substantially recovered only in A. donax after rewatering. Decline of photochemical and biochemical parameters, increased concentration of CO2 inside leaves, and impairment of chloroplast ultrastructure were only observed in H. macra indicating damage of photosynthetic machinery under drought. It is suggested that volatile and non-volatile isoprenoids produced by A. donax efficiently preserve the chloroplasts from transient drought damage, while H. macra invests on phenylpropanoids that are less efficient in preserving photosynthesis but likely offer better antioxidant protection under prolonged stress.

Topics: Abscisic Acid; Apigenin; Butadienes; Carotenoids; Chlorophyll; Chloroplasts; Coumaric Acids; Dehydration; Droughts; Ecosystem; Hemiterpenes; Luteolin; Pentanes; Photosynthesis; Poaceae; Water

The health of several East Antarctic moss-beds is declining as liquid water availability is reduced due to recent environmental changes. Consequently, a noninvasive and spatially explicit method is needed to assess the vigour of mosses spread throughout rocky Antarctic landscapes. Here, we explore the possibility of using near-distance imaging spectroscopy for spatial assessment of moss-bed health. Turf chlorophyll a and b, water content and leaf density were selected as quantitative stress indicators. Reflectance of three dominant Antarctic mosses Bryum pseudotriquetrum, Ceratodon purpureus and Schistidium antarctici was measured during a drought-stress and recovery laboratory experiment and also with an imaging spectrometer outdoors on water-deficient (stressed) and well-watered (unstressed) moss test sites. The stress-indicating moss traits were derived from visible and near infrared turf reflectance using a nonlinear support vector regression. Laboratory estimates of chlorophyll content and leaf density were achieved with the lowest systematic/unsystematic root mean square errors of 38.0/235.2 nmol g(-1) DW and 0.8/1.6 leaves mm(-1) , respectively. Subsequent combination of these indicators retrieved from field hyperspectral images produced small-scale maps indicating relative moss vigour. Once applied and validated on remotely sensed airborne spectral images, this methodology could provide quantitative maps suitable for long-term monitoring of Antarctic moss-bed health.

Topics: Antarctic Regions; Bryophyta; Chlorophyll; Chlorophyll A; Dehydration; Droughts; Geography; Imaging, Three-Dimensional; Plant Leaves; Spectrum Analysis; Stress, Physiological; Water

To evaluate the ecological adaptation mechanism of Haloxylon ammodendron and H. persicum from Ningxia, the host of Cistanche deserticola, the chlorophyll fluorescence under dehydration and diurnal variation was determined by IMAGING-PAM method. The results showed that H. ammodendron had higher photosynthetic electron transport activity (Fv/Fm), photosynthetic efficiency (qP), and PS II electron transport activity (ETR) than H. persicum. After 48 h dehydration, the chlorophyll fluorescence and water-retaining property of H. ammodendron were significantly higher than those of H. persicum. The significant difference in diurnal variation between H. ammo- dendron and H. persicum was observed and a 'V' trend was exhibited. It suggested that H. ammodendron had a stronger ability to adapt to the environment and had wider distribution, while H. persicum was limited by water and light and had narrow distribution.

Topics: Adaptation, Physiological; Amaranthaceae; Chlorophyll; Circadian Rhythm; Dehydration; Electron Transport; Fluorescence; Light; Photosynthesis; Plant Leaves; Water

Production per amount of water used (water use efficiency, WUE) is closely correlated with drought tolerance. Although stomatal aperture can regulate WUE, the underlying molecular mechanisms are still unclear. Previous reports revealed that stomatal closure was inhibited in the calcium-sensing receptor (CAS) antisense line of Arabidopsis (CASas). Here it is shown that decreased drought tolerance and WUE of CASas was associated with higher stomatal conductance due to improper regulation of stomatal aperture, rather than any change of stomatal density. CASas plants also had a lower CO2 assimilation rate that was attributed to a lower photosynthetic electron transport rate, leading to higher chlorophyll fluorescence. Gene co-expression combined with analyses of chlorophyll content and transcription levels of photosynthesis-related genes indicate that CAS is involved in the formation of the photosynthetic electron transport system. These data suggest that CAS regulates transpiration and optimizes photosynthesis by playing important roles in stomatal movement and formation of photosynthetic electron transport, thereby regulating WUE and drought tolerance.

Topics: Arabidopsis; Arabidopsis Proteins; Calcium; Calcium-Binding Proteins; Carbon Dioxide; Chlorophyll; Dehydration; Droughts; Electron Transport; Gene Expression Regulation, Plant; Models, Biological; Photosynthesis; Plant Epidermis; Plant Leaves; Plant Stomata; Plant Transpiration; Time Factors; Water

The impact of water-deficit stress on leaf cuticular waxes and cutin monomers, and traits associated with cuticle permeability were examined in Shandong and Yukon ecotypes of Eutrema salsugineum (syn. Thellungiella salsuginea). Although Shandong exhibits glaucous leaves, and Yukon is non-glaucous, wax amounts on non-stressed Yukon leaves were 4.6-fold higher than on Shandong, due mainly to Yukon's eightfold higher wax fatty acids, especially the C22 and C24 acid homologues. Water deficit caused a 26.9% increase in total waxes on Shandong leaves, due mainly to increased C22 and C24 acids; and caused 10.2% more wax on Yukon, due mainly to an increase in wax alkanes. Total cutin monomers on non-stressed leaves of Yukon were 58.3% higher than on Shandong. Water deficit caused a 28.2% increase in total cutin monomers on Shandong, whereas total cutin monomers were not induced on Yukon. With or without stress, more abundant cuticle lipids were generally associated with lower water loss rates, lower chlorophyll efflux rates and an extended time before water deficit-induced wilting. In response to water deficit, Shandong showed elevated transcription of genes encoding elongase subunits, consistent with the higher stress induction of acids by Shandong. Yukon's higher induction of CER1 and CER3 transcripts may explain why alkanes increased most on Yukon after water deficit. Eutrema, with its diverse cuticle lipids and responsiveness, provides a valuable genetic resource for identifying new genes and alleles effecting cuticle metabolism, and lays groundwork for studies of the cuticle's role in extreme stress tolerance.

Topics: Brassicaceae; Chlorophyll; Dehydration; Ecotype; Flowers; Gene Expression Regulation, Plant; Lipids; Membrane Lipids; Permeability; Plant Epidermis; Plant Leaves; Reverse Transcriptase Polymerase Chain Reaction

Resurrection plants can survive dehydration to air-dry state, thus they are excellent models of understanding drought and dehydration tolerance mechanisms. Haberlea rhodopensis, a chlorophyll-retaining resurrection plant, can survive desiccation to relative water content below 10%. Leaves, detached from plants of sun and shade habitats, were moderately (∼50%) dehydrated in darkness. During desiccation, chlorophyll a fluorescence was detected by the recently innovated wireless Intelligent FluoroSensor (IFS) chlorophyll fluorometer, working with three different detectors: a pulse-amplitude-modulated (PAM) broadband channel and two channels to measure non-modulated red and far-red fluorescence. No change in area-based chlorophyll content of leaves was observed. The maximal quantum efficiency of photosystem II decreased gradually in both shade and sun leaves. Shade leaves could not increase antennae-based quenching, thus inactivated photosystem II took part in quenching of excess irradiation. Sun leaves seemed to be pre-adapted to quench excess light as they established an intensive increase in antennae-based non-photochemical quenching parallel to desiccation. The higher far-red to red antennae-based quenching may sign light-harvesting complex reorganization. Thus, compared to PAM, IFS chlorophyll fluorometer has additional benefits including (i) parallel estimation of changes in the Chl content and (ii) prediction of underlying processes of excitation energy quenching.

Topics: Chlorophyll; Chlorophyll A; Dehydration; Ecosystem; Fluorescence; Fluorometry; Light; Plant Leaves; Tracheophyta

Dioon edule seedling mortality is mostly attributed to dehydration by prolonged drought, even when they present xeromorphic characteristics like the adult plants. The effect of germination date (GD) and soil water deficit on seedling tolerance to water stress was assessed. The seedlings germinated and grown from mature seeds every month from December to April GD were selected to evaluate the leaf area, photosynthetic pigment content, crassulacean acid metabolism (CAM) activity, stomatal conductance (gs) and leaflet anatomy at soil water potential (Ψs) of 0.0 MPa (day 1), -0.1 MPa (day 40), -1.0 MPa (day 90), -1.5 MPa (day 130), and a control (0.0 MPa at day 130) to recognize differences due to leaf development. The seedlings shifted from C3 to CAM cycling when exposed to water stress at Ψs of -1.0 MPa, like adult plants. The March-April GD seedlings with undeveloped sclerified hypodermis and stomata, presented reduced leaf area, lower Chlorophyll a/b ratio, higher CAM activity and midday partial stomatal closure when reached Ψs of -1.0 MPa. These have higher probability of dehydration during severe drought (February-April) than those of the December-February GD with similar Ψs. Plants used for restoration purposes must have full leaf development to increase the survival.

Topics: Adaptation, Physiological; Carotenoids; Chlorophyll; Chlorophyll A; Dehydration; Germination; Photosynthesis; Plant Stomata; Seedlings; Soil; Time Factors; Water; Zamiaceae

Water stress (WS) slows growth and photosynthesis (A(n)), but most knowledge comes from short-time studies that do not account for longer term acclimation processes that are especially relevant in tree species. Using two Eucalyptus species that contrast in drought tolerance, we induced moderate and severe water deficits by withholding water until stomatal conductance (g(sw)) decreased to two pre-defined values for 24 d, WS was maintained at the target g(sw) for 29 d and then plants were re-watered. Additionally, we developed new equations to simulate the effect on mesophyll conductance (g(m)) of accounting for the resistance to refixation of CO(2). The diffusive limitations to CO(2), dominated by the stomata, were the most important constraints to A(n). Full recovery of A(n) was reached after re-watering, characterized by quick recovery of gm and even higher biochemical capacity, in contrast to the slower recovery of g(sw). The acclimation to long-term WS led to decreased mesophyll and biochemical limitations, in contrast to studies in which stress was imposed more rapidly. Finally, we provide evidence that higher gm under WS contributes to higher intrinsic water-use efficiency (iWUE) and reduces the leaf oxidative stress, highlighting the importance of gm as a target for breeding/genetic engineering.

Topics: Carbon Dioxide; Carbon Isotopes; Cell Respiration; Chlorophyll; Chloroplasts; Dehydration; Electron Transport; Eucalyptus; Fluorescence; Mesophyll Cells; Mitochondria; Photosynthesis; Plant Stomata; Plant Transpiration; Quantum Theory; Species Specificity; Steam; Time Factors; Water

One of the most striking attributes of clonal plants is their capacity for physiological integration, which enables movement of essential resources between connected ramets. This study investigated the capacity of physiological integration to buffer differences in resource availability experienced by ramets of the clonal wild strawberry plant, Fragaria vesca. Specifically, a study was made of the responses of connected and severed offspring ramets growing in environments with different water availability conditions (well watered or water stressed) and nitrogen forms (nitrate or ammonium).. The experimental design consisted of three factors, 'integration' (connected, severed) 'water status' (well watered, water stressed) and 'nitrogen form' (nitrate, ammonium), applied in a pot experiment. The effects of physiological integration were studied by analysing photochemical efficiency, leaf spectral reflectance, photosynthesis and carbon and nitrogen isotope discrimination, the last of which has been neglected in previous studies.. Physiological integration buffered the stress caused by water deprivation. As a consequence, survival was improved in water-stressed offspring ramets that remained connected to their parent plants. The nitrogen isotope composition (δ(15)N) values in the connected water-stressed ramets were similar to those in ramets in the ammonium treatment; however, δ(15)N values in connected well-watered ramets were similar to those in the nitrate treatment. The results also demonstrated the benefit of integration for offspring ramets in terms of photochemical activity and photosynthesis.. This is the first study in which carbon and nitrogen isotopic discrimination has been used to detect physiological integration in clonal plants. The results for nitrogen isotope composition represent the first evidence of preferential transport of a specific form of nitrogen to compensate for stressful conditions experienced by a member clone. Water consumption was lower in plants supplied with ammonium than in plants supplied with nitrate, and therefore preferential transport of ammonium from parents to water-stressed offspring could potentially optimize the water use of the whole clone.

Topics: Analysis of Variance; Biological Transport; Biomass; Carbon Isotopes; Chlorophyll; Clone Cells; Dehydration; Fluorescence; Fragaria; Gases; Ions; Nitrogen; Nitrogen Isotopes; Photosynthesis; Plant Leaves; Reproduction, Asexual; Salts; Solutions; Time Factors

Recent studies have shown that tolerance to abiotic stress, including water stress, is improved by grafting. In a previous work, we took advantage of the natural variability of Capsicum spp. and selected accessions tolerant and sensitive to water stress as rootstocks. The behavior of commercial cultivar 'Verset' seedlings grafted onto the selected rootstocks at two levels of water stress provoked by adding 3.5 and 7% PEG (polyethylene glycol) was examined over 14 days. The objective was to identify the physiological traits responsible for the tolerance provided by the rootstock in order to determine if the tolerance is based on the maintenance of the water relations under water stress or through the activation of protective mechanisms. To achieve this goal, various physiological parameters were measured, including: water relations; proline accumulation; gas exchange; chlorophyll fluorescence; nitrate reductase activity; and antioxidant capacity. Our results indicate that the effect of water stress on the measured parameters depends on the duration and intensity of the stress level, as well as the rootstock used. Under control conditions (0% PEG) all plant combinations showed similar values for all measured parameters. In general terms, PEG provoked a strong decrease in the gas exchange parameters in the cultivar grafted onto the sensitive accessions, as also observed in the ungrafted plants. This effect was related to lower relative water content in the plants, provoked by an inefficient osmotic adjustment that was dependent on reduced proline accumulation. At the end of the experiment, chronic photoinhibition was observed in these plants. However, the plants grafted onto the tolerant rootstocks, despite the reduction in photosynthetic rate, maintained the protective capacity of the photosynthetic machinery mediated by osmotic adjustment (based on higher proline content). In addition, water stress limited uptake and further NO3(-) transfer to the leaves. Increased nitrate reductase activity in the roots was observed, mainly in plants grafted onto the sensitive rootstocks, as well as the ungrafted plants, and this was associated with the lessened flux to the leaves. This study suggests that PEG-induced water stress can be partially alleviated by using tolerant accessions as rootstocks.

Topics: Antioxidants; Capsicum; Chlorophyll; Dehydration; Lipid Peroxidation; Nitrate Reductase; Osmotic Pressure; Phenotype; Photosynthesis; Plant Leaves; Plant Proteins; Plant Roots; Polyethylene Glycols; Proline; Seedlings; Stress, Physiological; Time Factors; Water

Arabidopsis AtHB7 and AtHB12 transcription factors (TFs) belong to the homeodomain-leucine zipper subfamily I (HD-Zip I) and present 62% amino acid identity. These TFs have been associated with the control of plant development and abiotic stress responses; however, at present it is not completely understood how AtHB7 and AtHB12 regulate these processes.. By using different expression analysis approaches, we found that AtHB12 is expressed at higher levels during early Arabidopsis thaliana development whereas AtHB7 during later developmental stages. Moreover, by analysing gene expression in single and double Arabidopsis mutants and in transgenic plants ectopically expressing these TFs, we discovered a complex mechanism dependent on the plant developmental stage and in which AtHB7 and AtHB12 affect the expression of each other. Phenotypic analysis of transgenic plants revealed that AtHB12 induces root elongation and leaf development in young plants under standard growth conditions, and seed production in water-stressed plants. In contrast, AtHB7 promotes leaf development, chlorophyll levels and photosynthesis and reduces stomatal conductance in mature plants. Moreover AtHB7 delays senescence processes in standard growth conditions.. We demonstrate that AtHB7 and AtHB12 have overlapping yet specific roles in several processes related to development and water stress responses. The analysis of mutant and transgenic plants indicated that the expression of AtHB7 and AtHB12 is regulated in a coordinated manner, depending on the plant developmental stage and the environmental conditions. The results suggested that AtHB7 and AtHB12 evolved divergently to fine tune processes associated with development and responses to mild water stress.

Topics: Arabidopsis; Arabidopsis Proteins; Chlorophyll; Dehydration; Evolution, Molecular; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Genes, Duplicate; Glucuronidase; Homeodomain Proteins; Models, Biological; Photosynthesis; Plant Development; Plant Leaves; Plant Roots; Plant Stomata; Promoter Regions, Genetic; Seedlings; Seeds; Sequence Alignment; Stress, Physiological; Water

Resurrection plants usually grow in specific or extreme habitats and have the capacity to survive almost complete water loss. We characterized the physiological and biochemical responses of Paraisometrum mileense to extreme desiccation and found that it is a resurrection plant. We profiled the changes in lipid molecular species during dehydration and rehydration in P. mileense, and compared these with corresponding changes in the desiccation-sensitive plant Arabidopsis thaliana. One day of desiccation was lethal for A. thaliana but not for P. mileense. After desiccation and subsequent rewatering, A. thaliana showed dramatic lipid degradation accompanied by large increases in levels of phosphatidic acid (PA) and diacylglycerol (DAG). In contrast, desiccation and rewatering of P. mileense significantly decreased the level of monogalactosyldiacylglycerol and increased the unsaturation of membrane lipids, without changing the level of extraplastidic lipids. Lethal desiccation in P. mileense caused massive lipid degradation, whereas the PA content remained at a low level similar to that of fresh leaves. Neither damage nor repair processes, nor increases in PA, occurred during non-lethal desiccation in P. mileense. The activity of phospholipase D, the main source of PA, was much lower in P. mileense than in A. thaliana under control conditions, or after either dehydration or rehydration. It was demonstrated that low rates of phospholipase D-mediated PA formation in P. mileense might limit its ability to degrade lipids to PA, thereby maintaining membrane integrity following desiccation.

Topics: Arabidopsis; Chlorophyll; Craterostigma; Dehydration; Desiccation; Diglycerides; Malondialdehyde; Membrane Lipids; Phosphatidic Acids; Phospholipase D; Plant Leaves; Proline; Species Specificity; Stress, Physiological; Sucrose; Time Factors; Water

Different explants of fenugreek, T. foenum-graecum L. (Var. RMt-303), were compared for their callus induction and subsequent shoot regeneration capabilities on Murashige and Skoog media supplemented with different phytohormones in varying concentration. The highest percentage of callus induction frequency was observed in 1 ppm benzylaminopurine (BAP). Maximum shoots were induced on media supplemented with 0.5 ppm BAP using leaf and stem tissues as explants. However, root tissues showed only callusing with no subsequent shooting. Cotyledonary node responded better than hypocotyls in terms of shoot induction on media supplemented with thidiazuron (0.1 ppm). The callus was subjected to drought stress as simulated by reduced water potential of growth media due to addition of mannitol. Calli could withstand -2 MPa water potential till 30 days indicating that the drought stress tolerance mechanisms are functional in this variety. Chlorophyll a and b and total chlorophyll, proline and total phenolic contents, total peroxidase and catalase activities increased under stress conditions suggesting the tolerance of callus to drought stress. However, ascorbate peroxidase, guaiacol peroxidase activities were found to decrease slightly. Malondialdehyde and H2O2 contents were found to decrease while only a slight disturbance was found in membrane stability index. These results underline the mechanisms that are crucial for drought stress tolerance in fenugreek.

Topics: Adaptation, Physiological; Catalase; Chlorophyll; Culture Media; Dehydration; Droughts; Mannitol; Organoids; Oxidative Stress; Peroxidases; Phenols; Phenylurea Compounds; Plant Cells; Plant Leaves; Plant Proteins; Plant Shoots; Plants, Medicinal; Proline; Regeneration; Stress, Physiological; Thiadiazoles; Trigonella

Photosynthetic responses to persisting mild water stress were compared between the wild type (WT) and the respiratory complex I mutant CMSII of Nicotiana sylvestris. In both genotypes, plants kept at 80% leaf-RWC (WT80 and CMSII80) had lower photosynthetic activity and stomatal/mesophyll conductances compared to well-watered controls. While the stomatal conductance and the chloroplastic CO2 molar ratio were similar in WT80 and CMSII80 leaves, net photosynthesis was higher in CMSII80. Carboxylation efficiency was lowest in WT80 leaves both, on the basis of the same internal and chloroplastic CO2 molar ratio. Photosynthetic and fluorescence parameters indicate that WT80 leaves were only affected in the presence of oxygen. Photorespiration, as estimated by electron flux to oxygen, increased slightly in CMSII80 and WT80 leaves in accordance with increased glycerate contents but maximum photorespiration at low chloroplastic CO2 was markedly lowest in WT80 leaves. This suggests that carbon assimilation of WT80 leaves is impaired by limited photorespiratory activity. The results are discussed with respect to a possible pre-acclimation of complex I deficient leaves in CMSII to drive photosynthesis and photorespiration at low CO2 partial pressure.

Topics: Acclimatization; Carbon; Carbon Dioxide; Chlorophyll; Chloroplasts; Dehydration; Genotype; Light; Mitochondria; Mutation; Nicotiana; Oxygen; Photosynthesis; Photosystem II Protein Complex; Plant Leaves; Plant Stomata; Plant Transpiration; Water

In recent years, many studies have focused on the limiting role of mesophyll conductance (gm ) to photosynthesis (An ) under water stress, but no studies have examined the effect of drought on gm through the forest canopy. We investigated limitations to An on leaves at different heights in a mixed adult stand of sessile oak (Quercus petraea) and beech (Fagus sylvatica) trees during a moderately dry summer. Moderate drought decreased An of top and lowest beech canopy leaves much more than in leaves located in the mid canopy; whereas in oak, An of the lower canopy was decreased more than in sunlit leaves. The decrease of An was probably not due to leaf-level biochemistry given that VCmax was generally unaffected by drought. The reduction in An was instead associated with reduction in stomatal and mesophyll conductances. Drought-induced increases in stomatal limitations were largest in leaves from the top canopy, whereas drought-induced increases in mesophyll limitations were largest in leaves from the lowest canopy. Sensitivity analysis highlighted the need to decompose the canopy into different leaf layers and to incorporate the limitation imposed by gm when assessing the impact of drought on the gas exchange of tree canopies.

Topics: Carbon Dioxide; Chlorophyll; Dehydration; Droughts; Fagus; Fluorescence; Mesophyll Cells; Nitrogen; Photosynthesis; Plant Leaves; Plant Stomata; Quercus; Rain; Regression Analysis; Seasons; Temperature; Trees; Vapor Pressure; Water

Barley displays a great genetic diversity, constituting a valuable source to delineate the responses of contrasted genotypes to environmental constraints. Here, we investigated the level of oxidative stress and the participation of antioxidant systems in two barley genotypes: Express, a variety known to be sensitive to drought, and Saïda, an Algerian landrace selected for its tolerance to water deficit. Soil-grown 15-day-old plants were subjected to water deficit for 8 days and then rewatered. We observed that upon water stress Express exhibits compared to Saïda accelerated wilting and a higher level of oxidative stress evaluated by HPLC measurements of lipid peroxidation and by imaging techniques. In parallel, Express plants also display lower levels of catalase and superoxide dismutase activity. No great difference was observed regarding peroxiredoxins and methionine sulfoxide reductases, enzymes detoxifying peroxides and repairing oxidized proteins, respectively. In contrast, upon water stress and recovery, much higher contents and oxidation ratios of glutathione and ascorbate were measured in Express compared to Saïda. Express also shows during water deficit greater increases in the pools of lipophilic antioxidants like xantophyll carotenoids and α-tocopherol. Altogether, these data show that the differential behavior of the two genotypes involves distinct responses regarding antioxidant mechanisms. Indeed, the drought sensitivity of Express compared with Saïda is associated with oxidative damage and a lower enzymatic ROS-scavenging capacity, but in parallel with a much stronger enhancement of most mechanisms involving low-molecular weight antioxidant compounds.

Topics: Antioxidants; Ascorbic Acid; Carotenoids; Catalase; Chlorophyll; Dehydration; Droughts; Free Radical Scavengers; Genotype; Glutathione; Hordeum; Hydrogen Peroxide; Lipid Peroxidation; Oxidative Stress; Phenotype; Plant Leaves; Species Specificity; Stress, Physiological; Superoxide Dismutase; Tocopherols; Water

Organic pollutants effects on lichens have not been addressed. Rehydration is critical for lichens, a burst of free radicals involving NO occurs. Repeated dehydrations with organic pollutants could increase oxidative damage. Our aim is to learn the effects of cumene hydroperoxide (CP) during lichen rehydration using Ramalina farinacea (L.) Ach., its photobiont Trebouxia spp. and Asterochloris erici. Confocal imaging shows intracellular ROS and NO production within myco and phycobionts, being the chloroplast the main source of free radicals. CP increases ROS, NO and lipid peroxidation and reduces chlorophyll autofluorescence, although photosynthesis remains unaffected. Concomitant NO inhibition provokes a generalized increase of ROS and a decrease in photosynthesis. Our results suggest that CP induces a compensatory hormetic response in Ramalina farinacea that could reduce the lichen's antioxidant resources after repeated desiccation-rehydration cycles. NO is important in the protection from CP.

Topics: Air Pollutants; Benzene Derivatives; Chlorophyll; Dehydration; Desiccation; Lichens; Nitrogen Oxides; Oxidation-Reduction; Oxidative Stress; Photosynthesis

Chlorophyll fluorescence induction is widely applied to investigate plant growth conditions by calculating the ratio of its intensity at oxidized and reduced states. We examined the applicability of a time-resolved profile of chlorophyll fluorescence induction with the aid of multivariate analysis to monitor the leaf water stress. Principal component (PC) analysis of time-resolved images of chlorophyll fluorescence induction and their score images were reconstructed. Control leaves (non-stressed leaves) and water-stressed leaves could be classified by normalized PC3 score images. This technique has the potential to monitor the water stress condition of plants by using a simple device.

Topics: Araceae; Chlorophyll; Dehydration; Humidity; Image Processing, Computer-Assisted; Plant Leaves; Principal Component Analysis; Spectrometry, Fluorescence

It is unclear to what extent seasonal water stress impacts on plant productivity over Amazonia. Using new Greenhouse gases Observing SATellite (GOSAT) satellite measurements of sun-induced chlorophyll fluorescence, we show that midday fluorescence varies with water availability, both of which decrease in the dry season over Amazonian regions with substantial dry season length, suggesting a parallel decrease in gross primary production (GPP). Using additional SeaWinds Scatterometer onboard QuikSCAT satellite measurements of canopy water content, we found a concomitant decrease in daily storage of canopy water content within branches and leaves during the dry season, supporting our conclusion. A large part (r(2) = 0.75) of the variance in observed monthly midday fluorescence from GOSAT is explained by water stress over moderately stressed evergreen forests over Amazonia, which is reproduced by model simulations that include a full physiological representation of photosynthesis and fluorescence. The strong relationship between GOSAT and model fluorescence (r(2) = 0.79) was obtained using a fixed leaf area index, indicating that GPP changes are more related to environmental conditions than chlorophyll contents. When the dry season extended to drought in 2010 over Amazonia, midday basin-wide GPP was reduced by 15 per cent compared with 2009.

Topics: Chlorophyll; Dehydration; Environmental Monitoring; Fluorescence; Models, Biological; Photosynthesis; Plant Leaves; Seasons; South America; Spacecraft; Sunlight; Trees; Tropical Climate

Herbivore attack leads to resource conflicts between plant defensive strategies. Photoassimilates are required for defensive compounds and carbon storage below ground and may therefore be depleted or enriched in the roots of herbivore-defoliated plants. The potential role of belowground tissues as mediators of induced tolerance-defense trade-offs is unknown. We evaluated signaling and carbohydrate dynamics in the roots of Nicotiana attenuata following Manduca sexta attack. Experimental and natural genetic variability was exploited to link the observed metabolite patterns to plant tolerance and resistance. Leaf-herbivore attack decreased sugar and starch concentrations in the roots and reduced regrowth from the rootstock and flower production in the glasshouse and the field. Leaf-derived jasmonates were identified as major regulators of this root-mediated resource-based trade-off: lower jasmonate levels were associated with decreased defense, increased carbohydrate levels and improved regrowth from the rootstock. Application and transport inhibition experiments, in combination with silencing of the sucrose non-fermenting (SNF) -related kinase GAL83, indicated that auxins may act as additional signals that regulate regrowth patterns. In conclusion, our study shows that the ability to mobilize defenses has a hidden resource-based cost below ground that constrains defoliation tolerance. Jasmonate- and auxin-dependent mechanisms may lead to divergent defensive plant strategies against herbivores in nature.

Topics: Animals; Carbon; Chlorophyll; Cyclopentanes; Dehydration; Ecotype; Herbivory; Indoleacetic Acids; Manduca; Nicotiana; Oxylipins; Plant Leaves; Plant Proteins; Plant Roots; Signal Transduction

Long-lived shade leaves of avocado had extremely low rates of photosynthesis. Gas exchange measurements of photosynthesis were of limited use, so we resorted to Chl fluorescence imaging (CFI) and spot measurements to evaluate photosynthetic electron transport rates (ETRs) and non-photochemical quenching (NPQ). Imaging revealed a remarkable transient heterogeneity of NPQ during photosynthetic induction in these hypostomatous, heterobaric leaves, but was adequately integrated by spot measurements, despite long-lasting artifacts from repeated saturating flashes during assays. Major veins (mid-vein, first- and second-order veins) defined areas of more static large-scale heterogeneous NPQ, with more dynamic small-scale heterogeneity most strongly expressed in mesophyll cells between third- and fourth-order veins. Both responded to external CO2 concentration ([CO2]), occlusion of stomata with Vaseline™, leaf dehydration and relative humidity (RH). We interpreted these responses in terms of independent behavior of stomata in adjacent areoles that was largely expressed through CO2-limited photosynthesis. Heterogeneity was most pronounced and prolonged in the absence of net CO2 fixation in 100 p.p.m. [CO2] when respiratory and photorespiratory CO2 cycling constrained the inferred ETR to ~75% of values in 400 or 700 p.p.m. [CO2]. Likewise, sustained higher NPQ under Vaseline™, after dehydration or at low RH, also restricted ETR to ~75% of control values. Low NPQ in chloroplast-containing cells adjacent to major veins but remote from stomata suggested internal sources of high [CO2] in these tissues.

Topics: Carbon Dioxide; Chlorophyll; Dehydration; Electron Transport; Humidity; Light; Persea; Petrolatum; Photosynthesis; Plant Leaves; Plant Stomata; Plant Transpiration; Water; Xanthophylls

Cyclic electron flow (CEF) plays an important role in photoprotection for angiosperms under environmental stresses. However, ferns are more sensitive to drought and their water transport systems are not as efficient as those of angiosperms, it is unclear whether CEF also contributes to photoprotection in these plants. Using Microsorum punctatum and Paraleptochillus decurrens, we studied the electron fluxes through both photosystem I (PSI) and photosystem II (PSII) under water stress and their leaf anatomies. Our goal was to determine if CEF functions in the photoprotection of these ferns and, if so, whether CEF stimulation is related to leaf anatomy. Compared with P. decurrens, M. punctatum had thicker leaves and cuticles and higher water storage capacity, but lower stomatal density and slower rate of water loss. During induced drought, the decrease in leaf water potential (Ψ(leaf) ) was more pronounced in P. decurrens than in M. punctatum. For both species, the decline in Ψ(leaf) was associated with a lower effective PSII quantum yield, photochemical quantum yield of PSI and electron transport rate (ETR), whereas increases were found in the quantum yield of regulated energy dissipation, CEF and CEF/ETR(II) ratio. Values for CEF and the CEF/ETR(II) ratio peaked in M. punctatum at a light intensity of 500-600 µmol m(-2) s(-1) vs only 150-200 µmol m(-2) s(-1) in P. decurrens. Therefore, our results indicate that the stimulation of CEF in tropical ferns contributes to their photoprotection under water stress, and is related to their respective drought tolerance and leaf anatomy.

Topics: Biological Transport; Chlorophyll; Dehydration; Droughts; Electron Transport; Light; Photosynthesis; Photosystem I Protein Complex; Photosystem II Protein Complex; Plant Epidermis; Plant Leaves; Plant Stomata; Plant Transpiration; Polypodiaceae; Species Specificity; Stress, Physiological; Water

C3HC4-type RING zinc finger proteins are known to be essential in the regulation of plant processes, including responses to abiotic stress. Here, we identify, clone and examine the first C3HC4-type RING zinc finger protein (BrRZFP1) from Brassica rapa under stress conditions. Phylogenetic analysis of BrRZFP1 revealed strong sequence similarity to C3HC4-type zinc finger proteins from Arabidopsis that are induced by abiotic stresses. Diverse environmental stresses, including salt and cold, were found to induce BrRZFP1 transcripts greater than eightfold in B. rapa. Additional strong induction was shown of the stress hormone abscisic acid, together suggesting that BrRZFP1 could play a role as a general stress modulator. Similar profiles of induction for each of these stresses was found in both root and shoot tissues, although at much higher levels in roots. Constitutive expression of BrRZFP1 in Nicotiana tabacum was conducted to further analyse how changes in gene expression levels would affect plant stress responses. BrRZFP1 overexpression conferred increased tolerance to cold, salt and dehydration stresses. This was observed in several assays examining growth status throughout development, including increased germination, fresh weight and length of shoots and roots, as well as enhanced chlorophyll retention. These results suggest that the transcription factor BrRZFP1 is an important determinant of stress response in plants and that changes in its expression level in plants could increase stress tolerance.

Topics: Abscisic Acid; Adaptation, Physiological; Agrobacterium tumefaciens; Amino Acid Sequence; Base Sequence; Brassica rapa; Chlorophyll; Cloning, Molecular; Cold Temperature; Dehydration; Gene Expression Regulation, Plant; Genes, Plant; Germination; Molecular Sequence Data; Nicotiana; Phylogeny; Plant Proteins; Plant Roots; Plant Shoots; Plants, Genetically Modified; Recombinant Fusion Proteins; RING Finger Domains; Salt-Tolerant Plants; Seeds; Sodium Chloride; Stress, Physiological; Transcription Factors; Transcription, Genetic

Fremont cottonwood seedlings are vulnerable to water stress from rapid water-table decline during river recession in spring. Water stress is usually cited as the reason for reduced establishment, but interactions of water stress with microclimate extremes are more likely the causes of mortality. We assessed photosynthetic responses of Fremont cottonwood seedlings to water, light and heat stresses, which commonly co-occur in habitats where seedlings establish. Under moderate temperature and light conditions, water stress did not affect photosynthetic function. However, stomatal closure during water stress predisposed Fremont cottonwood leaves to light and heat stress, resulting in greatly reduced photosynthesis beginning at 31 °C versus at 41 °C for well-watered plants. Ontogenetic shifts in leaf orientation from horizontal to vertical, which occur as seedlings mature, reduce heat and light stress, especially during water stress. When compared with naturally occurring microclimate extremes, seedling stress responses suggest that reduced assimilation and photoprotection are common for Fremont cottonwood seedlings on exposed point bars where they establish. These reductions in photosynthesis likely have negative impacts on growth and may predispose young (<90-day-old) seedlings to early mortality during rapid water-table declines. Interactions with heat and light stress are more important in these effects than water stress alone.

Topics: Carbon Dioxide; Cell Respiration; Chlorophyll; Dehydration; Fluorescence; Hot Temperature; Light; Photosynthesis; Plant Leaves; Populus; Seasons; Seedlings; Water

Late Embryogenesis Abundant (LEA) proteins participate in plant stress responses and contribute to the acquisition of desiccation tolerance. In this report Rab28 LEA gene has been over-expressed in maize plants under a constitutive maize promoter. The expression of Rab28 transcripts led to the accumulation and stability of Rab28 protein in the transgenic plants. Native Rab28 protein is localized to nucleoli in wild type maize embryo cells; here we find by whole-mount immunocytochemistry that in root cells of Rab28 transgenic and wild-type plants the protein is also associated to nucleolar structures. Transgenic plants were tested for stress tolerance and resulted in sustained growth under polyethyleneglycol (PEG)-mediated dehydration compared to wild-type controls. Under osmotic stress transgenic seedlings showed increased leaf and root areas, higher relative water content (RWC), reduced chlorophyll loss and lower Malondialdehyde (MDA) production in relation to wild-type plants. Moreover, transgenic seeds exhibited higher germination rates than wild-type seeds under water deficit. Overall, our results highlight the presence of transgenic Rab28 protein in nucleolar structures and point to the potential of group 5 LEA Rab28 gene as candidate to enhance stress tolerance in maize plants.

Topics: Cell Nucleolus; Chlorophyll; Dehydration; Gene Expression; Gene Expression Regulation, Plant; Osmotic Pressure; Phenotype; Plant Leaves; Plant Proteins; Plant Roots; Plants, Genetically Modified; Promoter Regions, Genetic; Seedlings; Seeds; Water; Zea mays

Nitric oxide (NO) is a component of the repertoire of signals implicated in plant responses to environmental stimuli. In the present study, we investigated the effects of exogenous application of NO-releasing donor sodium nitroprusside (SNP) and nitric oxide synthase inhibitor N(G)-nitro-L-arginine-methyl ester (L-NAME) on dehydration and drought tolerance of Poncirus trifoliata. The endogenous NO level was enhanced by SNP pretreatment, but decreased by L-NAME, in the hydroponic or potted plants with or without stresses. Under dehydration, leaves from the SNP-treated hydroponic seedlings displayed less water loss, lower electrolyte leakage and reactive oxygen species accumulation, higher antioxidant enzyme activities and smaller stomatal apertures as compared with the control (treated with water). In addition, pretreatment of the potted plants with SNP resulted in lower electrolyte leakage, higher chlorophyll content, smaller stomatal conductance and larger photosynthetic rate relative to the control. By contrast, the inhibitor treatment changed these physiological attributes or phenotypes in an opposite way. These results indicate that NO in the form of SNP enhanced dehydration and drought tolerance, whereas the inhibitor makes the leaves or plants more sensitive to the stresses. The stress tolerance by NO might be ascribed to a combinatory effect of modulation of stomatal response and activation of the antioxidant enzymes. Taken together, NO is involved in dehydration and drought tolerance of P. trifoliata, implying that manipulation of this signal molecule may provide a practical approach to combat the environmental stresses.

Topics: Antioxidants; Catalase; Chlorophyll; Dehydration; Droughts; Electrolytes; Enzyme Inhibitors; Hydroponics; NG-Nitroarginine Methyl Ester; Nitric Oxide; Nitric Oxide Donors; Nitroprusside; Photosynthesis; Plant Stomata; Poncirus; Reactive Oxygen Species; Seedlings; Stress, Physiological; Superoxide Dismutase

Using chlorophyll (chl) fluorescence imaging, we studied the effect of mild (MiDS), moderate (MoDS) and severe (SDS) drought stress on photosystem II (PSII) photochemistry of 4-week-old Arabidopsis thaliana. Spatio-temporal heterogeneity in all chl fluorescence parameters was maintained throughout water stress. After exposure to drought stress, maximum quantum yield of PSII photochemistry (F(v)/F(m)) and quantum efficiency of PSII photochemistry (Φ(PSΙΙ)) decreased less in the proximal (base) than in the distal (tip) leaf. The chl fluorescence parameter F(v) /F(m) decreased less after MoDS than MiDS. Under MoDS, the antioxidant mechanism of A. thaliana leaves seemed to be sufficient in scavenging reactive oxygen species, as evident by the decreased lipid peroxidation, the more excitation energy dissipated by non-photochemical quenching (NPQ) and decreased excitation pressure (1-q(p)). Arabidopsis leaves appear to function normally under MoDS, but do not seem to have particular metabolic tolerance mechanisms under MiDS and SDS, as revealed by the level of lipid peroxidation and decreased quantum yield for dissipation after down-regulation in PSII (Φ(NPQ)), indicating that energy dissipation by down-regulation did not function and electron transport (ETR) was depressed. The simultaneous increased quantum yield of non-regulated energy dissipation (Φ(NO)) indicated that both the photochemical energy conversion and protective regulatory mechanism were insufficient. The non-uniform photosynthetic pattern under drought stress may reflect different zones of leaf anatomy and mesophyll development. The data demonstrate that the effect of different degrees of drought stress on A. thaliana leaves show spatio-temporal heterogeneity, implying that common single time point or single point leaf analyses are inadequate.

Topics: Antioxidants; Arabidopsis; Chlorophyll; Dehydration; Droughts; Electron Transport; Fluorescence; Lipid Peroxidation; Oxidative Stress; Photosystem II Protein Complex; Plant Leaves; Reactive Oxygen Species

Isoprenoid compounds synthesised in the plastids are involved in plant response to water deficit. The functionality of the biosynthetic pathway of these compounds under drought stress has been analysed at the physiological and molecular levels in two related species of tomato (Solanum chilense and Solanum lycopersicum) that differ in their tolerance to abiotic challenge. Expression analysis of the genes encoding enzymes of these pathways (DXS, IPI, GGPPS, PSY1, NCED and HPT1) in plants at different RWC values shows significant differences for only GGPPS and HPT1, with higher expression in the tolerant S. chilense. Chlorophyll, carotenoids, α-tocopherol and ABA content was also determined in both species under different drought conditions. In agreement with HPT1 transcriptional activity, higher α-tocopherol content was observed in S. chilense than in S. lycopersicum, which correlates with a lower degree of lipoperoxidation in the former species. These results suggest that, in addition to lower stomatal conductance, α-tocopherol biosynthesis is part of the adaptation mechanisms of S. chilense to adverse environmental conditions.

Topics: Abscisic Acid; Adaptation, Physiological; alpha-Tocopherol; Carotenoids; Chile; Chlorophyll; Dehydration; Droughts; Gene Expression Regulation, Plant; Genes, Plant; Lipid Peroxidation; Plant Stomata; Plastids; Solanum; Solanum lycopersicum; Terpenes; Transcription, Genetic

Drought stress is one of the most important factors that limit crop productivity worldwide. In order to obtain tomato plants with enhanced drought tolerance, we inserted the transcription factor gene ATHB-7 into the tomato genome. This gene was demonstrated earlier to be up-regulated during drought stress in Arabidopsis thaliana thus acting as a negative regulator of growth. We compared the performance of wild type and transgenic tomato line DTL-20, carrying ATHB-7 gene, under well-irrigated and water limited conditions. We found that transgenic plants had reduced stomatal density and stomatal pore size and exhibited an enhanced resistance to soil water deficit. We used the transgenic plants to investigate the potential of chlorophyll fluorescence to report drought tolerance in a simulated high-throughput screening procedure. Wild type and transgenic tomato plants were exposed to drought stress lasting 18 days. The stress was then terminated by rehydration after which recovery was studied for another 2 days. Plant growth, leaf water potential, and chlorophyll fluorescence were measured during the entire experimental period. We found that water potential in wild type and drought tolerant transgenic plants diverged around day 11 of induced drought stress. The chlorophyll fluorescence parameters: the non-photochemical quenching, effective quantum efficiency of PSII, and the maximum quantum yield of PSII photochemistry yielded a good contrast between wild type and transgenic plants from day 7, day 12, and day 14 of induced stress, respectively. We propose that chlorophyll fluorescence emission reports well on the level of water stress and, thus, can be used to identify elevated drought tolerance in high-throughput screens for selection of resistant genotypes.

Topics: Adaptation, Physiological; Chlorophyll; Dehydration; Droughts; Gene Expression Regulation, Plant; Gene Transfer Techniques; Genes, Plant; Genetic Variation; Plant Leaves; Plant Stomata; Plants, Genetically Modified; Solanum lycopersicum; Stress, Physiological; Transcription, Genetic

The relationships among photosynthetic acclimation, proline (Pro), soluble sugar (SS), and anthocyanin (An) accumulation in Arabidopsis thaliana leaves to the onset of drought stress (OnDS), mild (MiDS) and moderate drought stress (MoDS), were evaluated. As leaf water content (LWC) decreased, metabolic concentrations (Pro, SS, and An) increased and were negatively and significantly correlated with LWC. Thus, these metabolites may have an important role in the acclimation process to drought stress (DS). No correlations among Pro, SS and An accumulation with the quantum efficiency of PSII photochemistry (Φ(PSII)) and the excitation pressure (1-q(P)) were observed under DS. This implies that, while metabolites increased in a drought-dependent way, PSII activity did not decrease in the same pattern. Our results indicated that, under MoDS, A. thaliana leaves were able to maintain oxidative compounds such as malondialdeyde, an end product of lipid peroxidation, within the range of control leaves, and to cope with oxidative damage, as was evident by the decreased excitation pressure (1-q(P)) and similar (ns difference) Φ(PSII) to that of control leaves. In addition, a statistically significant increased accumulation of Pro, SS and An was recorded only under MoDS compared to controls. The better PSII functioning of MoDS Arabidopsis leaves may reflect the greater capacity of these leaves to undertake key metabolic adjustments, including increased Pro, SS and An accumulation, to maintain a higher antioxidant protection and a better balance between light capture and energy use.

Topics: Acclimatization; Anthocyanins; Arabidopsis; Carbohydrate Metabolism; Chlorophyll; Dehydration; Droughts; Fluorescence; Light; Lipid Peroxidation; Malondialdehyde; Oxidation-Reduction; Photosynthesis; Photosystem II Protein Complex; Plant Leaves; Proline; Quantum Theory; Soil; Solubility; Stress, Physiological; Thermodynamics; Water

The effect of magnetic field (MF) treatments of maize (Zea mays L.) var. Ganga Safed 2 seeds on the growth, leaf water status, photosynthesis and antioxidant enzyme system under soil water stress was investigated under greenhouse conditions. The seeds were exposed to static MFs of 100 and 200 mT for 2 and 1 h, respectively. The treated seeds were sown in sand beds for seven days and transplanted in pots that were maintained at -0.03, -0.2 and -0.4 MPa soil water potentials under greenhouse conditions. MF exposure of seeds significantly enhanced all growth parameters, compared to the control seedlings. The significant increase in root parameters in seedlings from magnetically-exposed seeds resulted in maintenance of better leaf water status in terms of increase in leaf water potential, turgor potential and relative water content. Photosynthesis, stomatal conductance and chlorophyll content increased in plants from treated seeds, compared to control under irrigated and mild stress condition. Leaves from plants of magnetically-treated seeds showed decreased levels of hydrogen peroxide and antioxidant defense system enzymes (peroxidases, catalase and superoxide dismutase) under moisture stress conditions, when compared with untreated controls. Mild stress of -0.2 MPa induced a stimulating effect on functional root parameters, especially in 200 mT treated seedlings which can be exploited profitably for rain fed conditions. Our results suggested that MF treatment (100 mT for 2 h and 200 for 1 h) of maize seeds enhanced the seedling growth, leaf water status, photosynthesis rate and lowered the antioxidant defense system of seedlings under soil water stress. Thus, pre sowing static magnetic field treatment of seeds can be effectively used for improving growth under water stress.

Topics: Catalase; Chlorophyll; Dehydration; Hydrogen Peroxide; Magnetic Fields; Peroxidases; Photosynthesis; Plant Leaves; Rain; Seedlings; Seeds; Soil; Superoxide Dismutase; Water; Zea mays

Large amounts of nitrogen (N) fertilizers are used in the production of oilseed rape. However, as low-input methods of crop management are introduced crops will need to withstand temporary N deficiency. In temperate areas, oilseed rape will also be affected by frequent drought periods. Here we evaluated the physiological and metabolic impact of nitrate limitation on the oilseed rape response to water deprivation. Different amounts of N fertilizer were applied to plants at the vegetative stage, which were then deprived of water and rehydrated. Both water and N depletion accelerated leaf senescence and reduced leaf development. N-deprived plants exhibited less pronounced symptoms of wilting during drought, probably because leaves were smaller and stomata were partially closed. Efficiency of proline production, a major stress-induced diversion of nitrogen metabolism, was assessed at different positions along the whole plant axis and related to leaf developmental stage and water status indices. Proline accumulation, preferentially in younger leaves, accounted for 25-85% of the free amino acid pool. This was mainly due to a better capacity for proline synthesis in fully N-supplied plants whether they were subjected to drought or not, as deduced from the expression patterns of the proline metabolism BnP5CS and BnPDH genes. Although less proline accumulated in the oldest leaves, a significant amount was transported from senescing to emerging leaves. Moreover, during rehydration proline was readily recycled. Our results therefore suggest that proline plays a significant role in leaf N remobilization and in N use efficiency in oilseed rape.

Topics: Biological Transport; Brassica napus; Chlorophyll; Dehydration; Gene Expression Regulation, Plant; Nitrogen; Phenotype; Phloem; Plant Leaves; Plant Proteins; Plant Roots; Plant Stomata; Plant Transpiration; Proline; RNA, Plant; Seedlings; Stress, Physiological; Water

The evergreen holm oak Quercus ilex L. is the most representative tree in Mediterranean forests. Accurate estimation of the limiting factors of photosynthesis for Q. ilex and the prediction of ecosystem water-use efficiency by mechanistic models can be achieved only by establishing whether this species shows heterogenic stomatal aperture, and, if so, the circumstances in which this occurs. Here, we collected gas-exchange and chlorophyll fluorescence data in Q. ilex leaves from a nursery to measure the effects of stomatal oscillations on PSII quantum yield (Φ(PSII)) under water stress. Stomatal conductance (g(s)) was used as an integrative indicator of the degree of water stress. Images of chlorophyll fluorescence showed heterogeneous Φ(PSII) when g(s) was <50 mmol H(2)O m(-2) s(-1), representative of severe drought and corresponding to a container capacity <45%. Stomatal patchiness was related to a coefficient of variation (CV) of Φ(PSII) values >2.5%. A parallel study in the forest confirmed heterogeneous Φ(PSII) values in leaves in response to declining water availability. Three kinds of Q. ilex individuals were distinguished: those resprouting after a clear-cut (resprouts, R); intact individuals growing in the same clear-cut area as resprouts (controls, C); and intact individuals in a nearby, undisturbed area (forest controls, CF). Patchiness increased in C and CF in response to increasing drought from early May to late July, whereas in R, Φ(PSII) values were maintained as a result of their improved water relations since the pre-existing roots were associated with a smaller aerial biomass. Patchiness was related to a % CV of Φ(PSII) values >4 and associated in the summer with mean g(s) values of 30 mmol H(2)O m(-2) s(-1). Under milder drought in spring, Φ(PSII) patchiness was less strictly related to g(s) variations, pointing to biochemical limitants of photosynthesis. The occurrence of heterogenic photosynthesis caused by patchy stomatal closure in Q. ilex during severe drought should be taken into account in ecosystem modelling in which harsher water stress conditions associated with climate change are predicted.

Topics: Chlorophyll; Climate; Dehydration; Droughts; Fluorescence; Gases; Mediterranean Region; Photosynthesis; Photosystem II Protein Complex; Plant Stomata; Quercus; Trees

Root chicory (Cichorium intybus var. sativum) is a cash crop cultivated for inulin production in Western Europe. This plant can be exposed to severe water stress during the last 3 months of its 6-month growing period. The aim of this study was to quantify the effect of a progressive decline in water availability on plant growth, photosynthesis, and sugar metabolism and to determine its impact on inulin production. Water stress drastically decreased fresh and dry root weight, leaf number, total leaf area, and stomatal conductance. Stressed plants, however, increased their water-use efficiency and leaf soluble sugar concentration, decreased the shoot-to-root ratio and lowered their osmotic potential. Despite a decrease in photosynthetic pigments, the photosynthesis light phase remained unaffected under water stress. Water stress increased sucrose phosphate synthase activity in the leaves but not in the roots. Water stress inhibited sucrose:sucrose 1-fructosyltransferase and fructan:fructan 1 fructosyltransferase after 19 weeks of culture and slightly increased fructan 1-exohydrolase activity. The root inulin concentration, expressed on a dry-weight basis, and the mean degree of polymerization of the inulin chain remained unaffected by water stress. Root chicory displayed resistance to water stress, but that resistance was obtained at the expense of growth, which in turn led to a significant decrease in inulin production.

Topics: Adaptation, Physiological; Carbohydrate Metabolism; Carbohydrates; Chlorophyll; Cichorium intybus; Dehydration; Droughts; Glucosyltransferases; Hexosyltransferases; Inulin; Photosynthesis; Plant Leaves; Plant Proteins; Plant Roots; Plant Shoots; Plant Stomata; Plant Transpiration; Water

In this study, we find and characterize the sources of tolerance to drought amongst an oat (Avena sativa L.) germplasm collection of 174 landraces and cultivars. We used multivariate analysis, non-supervised principal component analyses (PCA) and supervised discriminant function analyses (DFA) to suggest the key mechanism/s responsible for coping with drought stress. Following initial assessment of drought symptoms and area under the drought progress curve, a subset of 14 accessions were selected for further analysis. The collection was assessed for relative water content (RWC), cell membrane stability, stomatal conductance (g (1)), leaf temperature, water use efficiency (WUE), lipid peroxidation, lipoxygenase activity, chlorophyll levels and antioxidant capacity during a drought time course experiment. Without the use of multivariate approaches, it proved difficult to unequivocally link drought tolerance to specific physiological processes in the different resistant oat accessions. These approaches allowed the ranking of many supposed drought tolerance traits in the order of degree of importance within this crop, thereby highlighting those with a causal relationship to drought stress tolerance. Analyses of the loading vectors used to derive the PCA and DFA models indicated that two traits involved in water relations, temperature and RWC together with the area of drought curves, were important indicators of drought tolerance. However, other parameters involved in water use such as g (1) and WUE were less able to discriminate between the accessions. These observations validate our approach which should be seen as representing a cost-effective initial screen that could be subsequently employed to target drought tolerance in segregating populations.

Topics: Antioxidants; Avena; Cell Membrane; Chlorophyll; Dehydration; Droughts; Lipid Peroxidation; Lipoxygenases; Multifactorial Inheritance; Multivariate Analysis; Plant Leaves; Plant Stomata; Principal Component Analysis; Stress, Physiological; Temperature

The olive tree (Olea europaea L.) is often exposed to severe water stress during the summer season. In this study, we determined the changes in total phenol content, oleuropein and hydroxytyrosol in the leaves of four olive cultivars ('Gaidourelia', 'Kalamon', 'Koroneiki' and 'Megaritiki') grown under water deficit conditions for two months. Furthermore, we investigated the photosynthetic performance in terms of gas exchange and chlorophyll a fluorescence, as well as malondialdehyde content and antioxidant activity. One-year-old self-rooted plants were subjected to three irrigation treatments that received a water amount equivalent to 100% (Control, C), 66% (Field Capacity 66%, FC(66)) and 33% (Field Capacity 33%, FC(33)) of field capacity. Measurements were conducted 30 and 60 days after the initiation of the experiment. Net CO(2) assimilation rate, stomatal conductance and F(v)/F(m) ratio decreased only in FC(33) plants. Photosynthetic rate was reduced mainly due to stomatal closure, but damage to PSII also contributed to this decrease. Water stress induced the accumulation of phenolic compounds, especially oleuropein, suggesting their role as antioxidants. Total phenol content increased in FC(33) treatment and oleuropein presented a slight increase in FC(66) and a sharper one in FC(33) treatment. Hydroxytyrosol showed a gradual decrease as water stress progressed. Malondialdehyde (MDA) content increased due to water stress, mostly after 60 days, while antioxidant activity increased for all cultivars in the FC(33) treatment. 'Gaidourelia' could be considered as the most tolerant among the tested cultivars, showing higher phenolic concentration and antioxidant activity and lower lipid peroxidation and photochemical damage after two months of water stress. The results indicated that water stress affected olive tree physiological and biochemical parameters and magnitude of this effect depended on genotype, the degree of water limitation and duration of treatment. However, the severity as well as the duration of water stress might exceed antioxidant capacity, since MDA levels and subsequent oxidative damage increased after two months of water deficit.

Topics: Agricultural Irrigation; Antioxidants; Chlorophyll; Chlorophyll A; Dehydration; Genotype; Iridoid Glucosides; Iridoids; Lipid Peroxidation; Malondialdehyde; Olea; Oxidative Stress; Phenols; Phenylethyl Alcohol; Photosynthesis; Plant Leaves; Plant Roots; Plant Transpiration; Pyrans; Stress, Physiological

Dehydration responsive element binding proteins (DREB) are members of a larger family of transcription factors, many of which have been reported to contribute to plant responses to abiotic stresses in several species. While, little is known about their role in potato (Solanum tuberosum). This report describes the cloning and characterization of a DREB transcription factor cDNA, StDREB2, isolated from potato (cv Nicola) plants submitted to salt treatment. Based on a multiple sequence alignment, this protein was classified into the A-5 group of DREB subfamily. Expression studies revealed that StDREB2 was induced in leaves, roots and stems upon various abiotic stresses and in response to exogenous treatment with abscisic acid (ABA). In agreement with this expression pattern, over-expression of StDREB2 in transgenic potato plants resulted in enhanced tolerance to salt stress. These data suggest that the isolated StDREB2 encodes a functional protein involved in plant response to different abiotic stresses. An electrophoretic mobility shift assay (EMSA) indicated that the StDREB2 protein bound specifically to the DRE core element (ACCGAGA) in vitro. Moreover, Semi quantitative RT-PCR analysis revealed that the transcript level of a putative target gene i.e. δ(1)-pyrroline-5-carboxylate synthase (P5CS) was up-regulated in transgenic plants submitted to salt stress conditions. A concomitant increase in proline accumulation was also observed under these conditions. Taking together, all these data suggest that StDREB2 takes part in the processes underlying plant responses to abiotic stresses probably via the regulation of ABA hormone signaling and through a mechanism allowing proline synthesis.

Topics: Abscisic Acid; Amino Acid Sequence; Chlorophyll; Cold Temperature; Dehydration; Gene Expression Regulation, Plant; Molecular Sequence Data; Organ Specificity; Ornithine-Oxo-Acid Transaminase; Phylogeny; Plant Components, Aerial; Plant Growth Regulators; Plant Proteins; Plant Roots; Plants, Genetically Modified; Proline; Protein Binding; Salt Tolerance; Sequence Alignment; Signal Transduction; Solanum tuberosum; Stress, Physiological; Transcription Factors

The Populus sucrose (Suc) transporter 4 (PtaSUT4), like its orthologs in other plant taxa, is tonoplast localized and thought to mediate Suc export from the vacuole into the cytosol. In source leaves of Populus, SUT4 is the predominantly expressed gene family member, with transcript levels several times higher than those of plasma membrane SUTs. A hypothesis is advanced that SUT4-mediated tonoplast sucrose fluxes contribute to the regulation of osmotic gradients between cellular compartments, with the potential to mediate both sink provisioning and drought tolerance in Populus. Here, we describe the effects of PtaSUT4-RNA interference (RNAi) on sucrose levels and raffinose family oligosaccharides (RFO) induction, photosynthesis, and water uptake, retention and loss during acute and chronic drought stresses. Under normal water-replete growing conditions, SUT4-RNAi plants had generally higher shoot water contents than wild-type plants. In response to soil drying during a short-term, acute drought, RNAi plants exhibited reduced rates of water uptake and delayed wilting relative to wild-type plants. SUT4-RNAi plants had larger leaf areas and lower photosynthesis rates than wild-type plants under well-watered, but not under chronic water-limiting conditions. Moreover, the magnitude of shoot water content, height growth, and photosynthesis responses to contrasting soil moisture regimes was greater in RNAi than wild-type plants. The concentrations of stress-responsive RFOs increased in wild-type plants but were unaffected in SUT4-RNAi plants under chronically dry conditions. We discuss a model in which the subcellular compartmentalization of sucrose mediated by PtaSUT4 is regulated in response to both sink demand and plant water status in Populus.

Topics: Biomass; Chlorophyll; Dehydration; Droughts; Gases; Membrane Transport Proteins; Photosynthesis; Plant Leaves; Plant Proteins; Plant Stems; Plants, Genetically Modified; Populus; Raffinose; RNA Interference; Soil; Sucrose; Vacuoles; Water

Drought is the major environmental factor limiting crop productivity worldwide. We hypothesized that it is possible to enhance drought tolerance by delaying stress-induced senescence through the stress-induced synthesis of cytokinins in crop-plants. We generated transgenic rice (Oryza sativa) plants expressing an isopentenyltransferase (IPT) gene driven by P(SARK) , a stress- and maturation-induced promoter. Plants were tested for drought tolerance at two yield-sensitive developmental stages: pre- and post-anthesis. Under both treatments, the transgenic rice plants exhibited delayed response to stress with significantly higher grain yield (GY) when compared to wild-type plants. Gene expression analysis revealed a significant shift in expression of hormone-associated genes in the transgenic plants. During water-stress (WS), P(SARK)::IPT plants displayed increased expression of brassinosteroid-related genes and repression of jasmonate-related genes. Changes in hormone homeostasis were associated with resource(s) mobilization during stress. The transgenic plants displayed differential expression of genes encoding enzymes associated with hormone synthesis and hormone-regulated pathways. These changes and associated hormonal crosstalk resulted in the modification of source/sink relationships and a stronger sink capacity of the P(SARK)::IPT plants during WS. As a result, the transgenic plants had higher GY with improved quality (nutrients and starch content).

Topics: Alkyl and Aryl Transferases; Biomass; Chlorophyll; Cytokinins; Dehydration; Droughts; Edible Grain; Gene Expression Profiling; Gene Expression Regulation, Plant; Homeostasis; Oryza; Plant Growth Regulators; Plant Leaves; Plant Proteins; Plants, Genetically Modified; Promoter Regions, Genetic; Seedlings; Starch; Sucrose; Time Factors

Vitamin E has been found to be associated with an important antioxidant property in mammals and plants. In photosynthetic organisms, the enzyme 4-hydroxyphenylpyruvate dioxygenase (HPPD; E.C. 1.13.11.27) plays an important role in the vitamin E biosynthetic pathway. The full-length cDNA encoding HPPD was isolated from Lactuca sativa L. by rapid amplification of cDNA ends (RACE). The cDNA, designated as LsHPPD, was 1743 base pairs (bp) long containing an open reading frame (ORF) of 1338 bp encoding a protein of 446 amino acids. Sequence analysis indicated that LsHPPD shared high identity with HPPD from Medicago truncatula L. Real-time fluorescent quantitative PCR (qPCR) analysis revealed that LsHPPD was preferentially expressed in mature leaves compared with other tissues and that the LsHPPD expression was sensitive to high light and drought stress treatments. Transient expression of LsHPPD via agroinfiltration resulted in 12-fold increase in LsHPPD mRNA expression level and 4-fold enhancement in α-tocopherol content compared with the negative control. A decrease in chlorophyll content and inhibition of photosystem II were observed during stress treatments and agroinfiltration.

Topics: 4-Hydroxyphenylpyruvate Dioxygenase; Amino Acid Sequence; Base Sequence; Chlorophyll; Cloning, Molecular; Dehydration; DNA, Complementary; Gene Expression Regulation, Plant; Lactuca; Light; Molecular Sequence Data; Phylogeny; Plant Leaves; Plant Roots; Plant Stems; Real-Time Polymerase Chain Reaction; RNA, Plant; Sequence Alignment; Sequence Analysis, DNA; Stress, Physiological; Vitamin E

Paper mulberry (Broussonetia papyrifera L. Vent) is well known for its bark fibers, which are used for making paper, cloth, rope, etc. It was found that, in addition to its well-documented role in the enhancement of plant salt tolerance, overexpression of the Na+/H+ antiporter (AtNHX5) gene in paper mulberry plants showed high drought tolerance. After exposure to water deficiency and salt stress, the wild-type (WT) plants all died, while the AtNHX5-overexpressing plants remained alive under high salt stress, and had a higher survival rate (>66%) under drought stress. Measurements of ion levels indicated that Na+ and K+ contents were all higher in AtNHX5-overexpressing leaves than in WT leaves in high saline conditions. The AtNHX5 plants had higher leaf water content and leaf chlorophyll contents, accumulated more proline and soluble sugars, and had less membrane damage than the WT plants under water deficiency and high saline conditions. Taken together, the results indicate that the AtNHX5 gene could enhance the tolerance of paper mulberry plants to multiple environmental stresses by promoting the accumulation of more effective osmolytes (ions, soluble sugars, proline) to counter the osmotic stress caused by abiotic factors.

Topics: Arabidopsis; Arabidopsis Proteins; Broussonetia; Carbohydrates; Chlorophyll; Dehydration; Droughts; Gene Expression; Plant Leaves; Plants, Genetically Modified; Potassium; Proline; Salt Tolerance; Sodium; Sodium-Hydrogen Exchangers

Proline accumulation is responsible for stress adaptation in many plants. To distinguish the involvement of two proline synthetic pathways, the virus induced gene silencing (VIGS) system that silenced the expression of genes encoding Δ(1)-pyrroline-5-carboxylate synthetase (P5CS; EC:1.5.1.12) and ornithine-δ-aminotransferase (OAT; EC 2.6.1.13) was performed, separately or concomitantly, in four-week-old Nicotiana benthamiana. Leaf discs of VIGS-treated tobacco were subjected to the treatment of drought, abscisic acid (ABA), or polyethylene glycol (PEG). The treated leaf discs were then collected for the determination of mRNA, chlorophyll, proline and polyamine level. Under drought stress or PEG treatment, most proline accumulation was inhibited in P5CS-silenced plants and only a small portion was inhibited in OAT-silenced plants under drought stress and no inhibition was observed under PEG treatment. Under ABA treatment, proline accumulation was inhibited completely in P5CS-silenced plants but unaffected in OAT-silenced plants. The degradation of chlorophyll was enhanced in P5CS-silenced plants but retarded in OAT-silenced plants under PEG treatment. Under ABA treatment, the degradation of chlorophyll was unaffected in both P5CS-silenced and OAT-silenced plants. The increase of polyamine level was unaffected in P5CS-silenced plants but increased in OAT-silenced plants under PEG treatment. Under ABA treatment, the increase of polyamine level was unaffected in P5CS-silenced plants but the polyamine level was increased later in OAT-silenced plants. Therefore, P5CS plays a major role in proline accumulation under drought, PEG, or ABA treatment, while OAT plays a minor role in drought or PEG treatment and does not participate in ABA treatment. OAT appears to have a close relationship with the regulation of polyamine levels in PEG and ABA treatments.

Topics: 1-Pyrroline-5-Carboxylate Dehydrogenase; Abscisic Acid; Biosynthetic Pathways; Chlorophyll; Cloning, Molecular; Dehydration; Droughts; Gene Expression Regulation, Plant; Gene Silencing; Genes, Plant; Genetic Vectors; Glutamate-5-Semialdehyde Dehydrogenase; Multienzyme Complexes; Nicotiana; Ornithine-Oxo-Acid Transaminase; Phosphotransferases (Alcohol Group Acceptor); Plant Leaves; Polyamines; Polyethylene Glycols; Proline; RNA, Messenger; Stress, Physiological; Tobacco Mosaic Virus

Functional characterization of water deficit stress responsive genes is important to understand their role in stress tolerance. RNAi-based silencing of gene of interest and studying the stress response of knockdown plants under stress can be one of the potential options for assessing functional significance of these genes. Several genes showing higher transcript expression under water deficit stress were cloned earlier from a stress adapted crop species, groundnut. In this study, a few selected gene homologs have been characterized in Nicotiana tabacum and Arabidopsis. Using post transcriptional gene silencing (PTGS) based RNAi approach we developed N. tabacum knockdown lines for three of the genes namely alcohol dehydrogenase (ADH), trans caffeoyl coA-3-O-methyl transferase (CcoAOMT) and flavonol-3-O-glucosyl transferase (F3OGT). By quantitative RT-PCR we demonstrated that the RNAi lines showed significant reduction in target gene transcripts. We followed a stress imposition protocol that allows the plants to experience initial gradual acclimation stress and subsequently severe stress for a definite period. The RNAi knockdown lines generated against ADH and F3OGT, when subjected to water deficit stress showed susceptible symptoms signifying the relevance of these genes under stress. Knockdown of CcoAOMT showed higher chlorophyll degradation and less cell viability upon stress compared to control plants. Further, the Arabidopsis mutant lines clearly showed susceptibility to salinity and water deficit stresses validating relevance of these three genes under abiotic stresses.

Topics: Acclimatization; Adaptation, Physiological; Alcohol Dehydrogenase; Arabidopsis; Arachis; Cell Survival; Chlorophyll; Cloning, Molecular; Dehydration; Desiccation; Down-Regulation; Gene Expression; Gene Expression Regulation, Plant; Genes, Plant; Glucosyltransferases; Methyltransferases; Mutation; Nicotiana; RNA Interference; Salinity; Sequence Homology; Stress, Physiological

Two soybean cultivars, one conventional and a glyphosate-tolerant (transgenic), were submitted to the water stress and the chlorophyll a fluorescence induced by UV light was monitored daily during 16 days. In this work, 40 pots in total, 20 per cultivar were used in the investigation. Each cultivar was divided in two groups, the control group and the group submitted to the water stress. The stress response of the cultivars was monitored by red to far-red fluorescence ratio. The data indicate that the water stress induced the earliest changes on the fluorescence ratio and chlorophyll content for the conventional cultivar. In addition, a comparative analysis of the fluorescence ratios of the cultivars reveals that conventional plants have higher chlorophyll content than transgenic ones. This result might be useful in the development of methodologies able to distinguish conventional to transgenic apart.

Topics: Chlorophyll; Chlorophyll A; Dehydration; Fluorescence; Glycine max; Plants, Genetically Modified; Water

*Heterosis increases yield potential and improves adaptation to stress in maize (Zea mays); however, the underlying mechanisms remain elusive. *A set of tropical inbred lines and their hybrids were grown in the field for 2 yr under three different water regimes. First-year plant water use was evaluated by measuring instantaneous traits (stomatal conductance (g(s)) and steady-state chlorophyll fluorescence (F(s))) in individual leaves together with time-integrative traits, which included mineral accumulation in the whole leaves of plants and oxygen isotope enrichment above source water (Delta(18)O) and carbon isotope discrimination (Delta(13)C) in the same pooled leaves and in mature kernels. Second-year water use was evaluated by measuring leaf temperature, g(s) and relative water content (RWC). *Within each growing condition, hybrids showed higher F(s), mineral accumulation, RWC, and lower leaf temperature, Delta(18)O and Delta(13)C than inbred lines. Therefore, hybrids had a better water status than inbred lines, regardless of the water conditions. Differences in grain yield across growing conditions were explained by differences in water-use traits, with hybrids and inbred lines following a common pattern. Within each growing condition, most variations in grain yield, between hybrids and inbred lines, were also explained by differences in plant water-use traits. *Heterosis in tropical maize seems to be mediated by improved water use, irrespective of the water conditions during growth.

Topics: Air; Biomass; Chlorophyll; Crops, Agricultural; Dehydration; Environment; Hybrid Vigor; Hybridization, Genetic; Inbreeding; Nitrogen; Quantitative Trait, Heritable; Temperature; Water; Zea mays

Using cryo-SEM with EDX fundamental structural and mechanical properties of the moss Ceratodon purpureus (Hedw.) Brid. were studied in relation to tolerance of freezing temperatures. In contrast to more complex plants, no ice accumulated within the moss during the freezing event. External ice induced desiccation with the response being a function of cell type; water-filled hydroid cells cavitated and were embolized at -4 °C while parenchyma cells of the inner cortex exhibited cytorrhysis, decreasing to ∼ 20% of their original volume at a nadir temperature of -20 °C. Chlorophyll fluorescence showed that these winter acclimated mosses displayed no evidence of damage after thawing from -20 °C while GCMS showed that sugar concentrations were not sufficient to confer this level of freezing tolerance. In addition, differential scanning calorimetry showed internal ice nucleation occurred in hydrated moss at ∼-12 °C while desiccated moss showed no evidence of freezing with lowering of nadir temperature to -20 °C. Therefore the rapid dehydration of the moss provides an elegantly simple solution to the problem of freezing; remove that which freezes.

Topics: Adaptation, Physiological; Australia; Bryopsida; Chlorophyll; Cryoelectron Microscopy; Dehydration; Freezing; Ice; Mesophyll Cells; Temperature; Water

Strategies such as foliar application of antitranspirants have the potential to regulate transpiration, but often, the limitation of CO(2) exchange as a result of reduced stomatal conductance can impair this beneficial effect. Elevated ambient [CO(2)] could significantly improve CO(2) diffusion while effectively reducing transpiration. In this experiment, we examined the response of sweet pepper (Capsicum annuum L.) to the foliar application of antitranspirant (AT) under two [CO(2)] (380 and 2000 micromol mol(-1)) and two drought intensities (4 or 8d without irrigation). The results showed that stomatal conductance and transpiration were reduced, while AT impaired photosynthesis at standard, but not at elevated [CO(2)] of fully irrigated plants. This effect was already apparent after 4d of drought. Drought had a minor impact on chlorophyll fluorescence (F(v)/F(m)). Additionally, root respiration was increased at elevated [CO(2)] but, after 8d of drought, it was higher for plants treated with AT than for non-sprayed plants. Leaf water potential was affected more by drought at ambient compared to elevated [CO(2)], and, especially after 8d of drought, AT minimized the reductions in leaf water potential. Leaf concentrations of proline and starch were affected by both [CO(2)] and AT, especially after 8d of drought. Moreover, increasing [CO(2)] promoted the accumulation of starch, but led to decreases in the tissue concentrations of the soluble organic osmolytes, and hence diminished osmotic adjustment after 8d of water withholding, relative to ambient [CO(2)]. This study indicates that, in addition to the reported beneficial effect of elevated [CO(2)] on drought stress, AT could significantly improve drought tolerance in sweet pepper plants.

Topics: Amino Acids; Capsicum; Carbohydrate Metabolism; Carbon Dioxide; Cell Respiration; Chlorophyll; Dehydration; Fluorescence; Photosynthesis; Plant Leaves; Plant Roots; Plant Transpiration; Solubility; Starch; Water

Cassava (Manihot esculenta Crantz) sheds its leaves during growth, especially within the tropical dry season. With the production of SAG12-IPT transgenic cassava we want to test the level of leaf retention and altered cytokinin metabolism of transgenic plants via the autoregulatory senescence inhibition system. After confirmation of transgene expression by molecular analysis and phenotype examination in greenhouse plants, two transgenic plant lines, 529-28 and 529-48, were chosen for further investigation. Detached mature leaves of 529-28 plants retained high levels of chlorophyll compared with wild-type leaves after dark-induced senescence treatment. Line 529-28 showed significant drought tolerance as indicated by stay-green capacity after drought stress treatment. Field experiments proved that leaf senescence syndrome was significantly delayed in 529-28 plants in comparison with wild-type and 529-48 plants. Physiological and agronomical characterizations of these plants also revealed that the induced expression of IPT had effects on photosynthesis, sugar allocation and nitrogen partitioning. Importantly, the 529-28 plants accumulated a high level of trans-zeatin-type cytokinins particularly of corresponding storage O-glucosides to maintain cytokinin homeostasis. Our study proves the feasibility of prolonging the leaf life of woody cassava and also sheds light on the control of cytokinin homeostasis in cassava leaves.

Topics: Alkyl and Aryl Transferases; Cellular Senescence; Chlorophyll; Cytokinins; Dehydration; Droughts; Gene Expression Regulation, Plant; Manihot; Plant Leaves; Plants, Genetically Modified

Drought and salinity induce water deficit, but may also have distinct effects on plant metabolism. To compare their impact on leaf senescence in relation to ABA and ethylene synthesis, young plants of Atriplex halimus L. were exposed to iso-osmotic concentrations of NaCl (160mM) or PEG (15%) in nutrient solution. Plant growth and development were more affected by PEG than by NaCl. Stressed plants remained able to reduce their osmotic potential, but the nature of accumulated organic osmocompatible solutes varied according to the stressing agent. Glycinebetaine accumulated to a greater extent in salt-treated plants than in water-stressed plants. Sodium chloride induced the accumulation of non-reducing sucrose, while PEG-treated plants mainly accumulated reducing glucose and fructose. Abscisic acid (ABA) accumulated in response to salt, while ethylene was synthesized mainly by PEG-treated plants and was involved in the induction of early senescence processes characterized by synthesis of reactive oxygen species, peroxidation of membrane lipids and a decrease in chlorophyll content. ABA sensitivity of stressed tissues was markedly different in response to salt and in response to non-ionic osmotic stress, and exogenous ABA (50μM) had contrasting effects on most physiological parameters depending on the stressing agent. Exogenous ABA induced a decrease in root and shoot growth and sucrose content, and an increase in reactive oxygen species content in salt-stressed plants. In contrast, exogenous ABA increased growth in PEG-treated plants in relation to an improvement of water use efficiency resulting from a more efficient stomatal control. Exogenous ABA increased ethylene synthesis in salt-treated plants, but had only marginal impact on PEG-treated ones. The xero-halophyte A. halimus thus responds in a contrasting way to salt and water stress, through accumulation of distinct osmocompatible solutes and hormonal compounds such as ethylene and ABA could play distinct roles in stress-induced senescence processes.

Topics: Abscisic Acid; Atriplex; Biomass; Cellular Senescence; Chlorophyll; Dehydration; Ethylenes; Hexoses; Hydrogen Peroxide; Malondialdehyde; Osmolar Concentration; Oxidative Stress; Plant Leaves; Plant Shoots; Plant Stomata; Salinity; Superoxides

As there is limited information on the mechanisms of vegetative desiccation tolerance in pteridophytes, we undertook a comprehensive anatomical, ultrastructural, physiological and biochemical study on the fern Mohria caffrorum. Our data show that this species is desiccation-tolerant during the dry season, and desiccation-sensitive in the rainy season. This system allows the verification of protection mechanisms by comparison of tolerant and sensitive tissues of the same species at the same developmental age. Tolerant fronds acquire protection mechanisms during drying that are mostly similar to those reported for angiosperms. These include: (i) chlorophyll masking by abaxial scales and frond curling; (ii) increased antioxidant capacity that is maintained in dry tissues; (iii) mechanical stabilization of vacuoles in the dry state; (iv) de novo production of heat stable proteins (at least one identified as a putative chaperonin); (v) accumulation of protective carbohydrates (sucrose, raffinose family oligosaccharides and cyclitols). This study has implications for the biotechnological production of drought-tolerant crops, and allows speculation on the evolution of vegetative desiccation tolerance.

Topics: Adaptation, Physiological; Antioxidants; Carbohydrates; Chlorophyll; Dehydration; Droughts; Ecosystem; Ferns; Plant Leaves; Seasons; South Africa; Water

Peanut genotypes from the US mini-core collection were analysed for changes in leaf proteins during reproductive stage growth under water-deficit stress. One- and two-dimensional gel electrophoresis (1- and 2-DGE) was performed on soluble protein extracts of selected tolerant and susceptible genotypes. A total of 102 protein bands/spots were analysed by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and by quadrupole time-of-flight tandem mass spectrometry (Q-TOF MS/MS) analysis. Forty-nine non-redundant proteins were identified, implicating a variety of stress response mechanisms in peanut. Lipoxygenase and 1l-myo-inositol-1-phosphate synthase, which aid in inter- and intracellular stress signalling, were more abundant in tolerant genotypes under water-deficit stress. Acetyl-CoA carboxylase, a key enzyme of lipid biosynthesis, increased in relative abundance along with a corresponding increase in epicuticular wax content in the tolerant genotype, suggesting an additional mechanism for water conservation and stress tolerance. Additionally, there was a marked decrease in the abundance of several photosynthetic proteins in the tolerant genotype, along with a concomitant decrease in net photosynthesis in response to water-deficit stress. Differential regulation of leaf proteins involved in a variety of cellular functions (e.g. cell wall strengthening, signal transduction, energy metabolism, cellular detoxification and gene regulation) indicates that these molecules could affect the molecular mechanism of water-deficit stress tolerance in peanut.

Topics: Acetyl-CoA Carboxylase; Arachis; Chlorophyll; Dehydration; Electrophoresis, Gel, Two-Dimensional; Gene Expression Regulation, Plant; Genotype; Myo-Inositol-1-Phosphate Synthase; Photosynthesis; Plant Proteins; Plant Transpiration; Proteome; Proteomics; RNA, Plant; Stress, Physiological; Tandem Mass Spectrometry; Water

We tested the hypothesis that Eucalyptus globulus Labill. genotypes that are more resistant to dry environments might also exhibit higher cold tolerances than drought-sensitive plants. The effect of low temperatures was evaluated in acclimated and unacclimated ramets of a drought-resistant clone (CN5) and a drought-sensitive clone (ST51) of E. globulus. We studied the plants' response via leaf gas exchanges, leaf water and osmotic potentials, concentrations of soluble sugars, several antioxidant enzymes and leaf electrolyte leakage. Progressively lowering air temperatures (from 24/16 to 10/-2 degrees C, day/night) led to acclimation of both clones. Acclimated ramets exhibited higher photosynthetic rates, stomatal conductances and lower membrane relative injuries when compared to unacclimated ramets. Moreover, low temperatures led to significant increases of soluble sugars and antioxidant enzymes activity (glutathione reductase, ascorbate peroxidase and superoxide dismutases) of both clones in comparison to plants grown at control temperature (24/16 degrees C). On the other hand, none of the clones, either acclimated or not, exhibited signs of photoinhibition under low temperatures and moderate light. The main differences in the responses to low temperatures between the two clones resulted mainly from differences in carbon metabolism, including a higher accumulation of soluble sugars in the drought-resistant clone CN5 as well as a higher capacity for osmotic regulation, as compared to the drought-sensitive clone ST51. Although membrane injury data suggested that both clones had the same inherent freezing tolerance before and after cold acclimation, the results also support the hypothesis that the drought-resistant clone had a greater cold tolerance at intermediate levels of acclimation than the drought-sensitive clone. A higher capacity to acclimate in a short period can allow a clone to maintain an undamaged leaf surface area along sudden frost events, increasing growth capacity. Moreover, it can enhance survival chances in frost-prone sites expanding the plantation range with more adaptive clones.

Topics: Acclimatization; Antioxidants; Carbohydrate Metabolism; Chlorophyll; Cold Temperature; Dehydration; Droughts; Electrolytes; Eucalyptus; Freezing; Osmosis; Photosynthesis; Plant Leaves; Plant Stomata; Stress, Physiological; Trees; Water

Refugia of mixed beech forest persist in the central mountains of the Iberian Peninsula at the south-western limit of European beech (Fagus sylvatica L.) distribution. The lack of beech regeneration is a concern in this region that has experienced reduced rainfall and higher temperatures over the past 30 years. Beech is considered especially susceptible to climate change because of its conservative shade-tolerant growth strategy; hence seedling responses to drought stress in gaps and in the understory are of particular interest. During the summer of 2007, a watering treatment raised the soil water content by up to 5% in gap and understory plots of beech seedlings in a mixed beech forest. Root-collar diameter was increased by our watering treatment in understory seedlings. Neither drought-avoidance through stomatal closure nor physiological drought-tolerance mechanisms were able to mitigate the effects of water stress in the understory seedlings, whereas osmotic adjustment enhanced the ability of the gap seedlings to tolerate water stress. Overall, high photosynthetic rates in the gaps, despite the photoinhibitory effects of high radiation, allowed gap seedlings to survive and grow better than the understory seedlings irrespective of water availability. Our results indicate that further intensification of summer drought, predicted for the Iberian Peninsula, will hinder the establishment of a beech seedling bank in the understory because of the conflicting seedling trait responses to simultaneously withstand water stress and to tolerate shade.

Topics: Adaptation, Physiological; Carbon Dioxide; Chlorophyll; Dehydration; Droughts; Fagus; Light; Photosynthesis; Plant Stomata; Seasons; Seedlings; Stress, Physiological; Trees; Water

The paper reports the effects of selenium (Se) supply on growth and some physiological traits of wheat (Triticum aestivum L. cv Shijiazhuang NO. 8) seedlings exposed to drought stress. The growth and physiological responses of seedlings were different depending on the Se concentration. The higher (3.0 mg Se kg(-1)) and lower amount used (0.5 mg Se kg(-1)) did not significantly affect on biomass accumulation. Treatments with 1.0 and 2.0 mg Se kg(-1) promoted biomass accumulation of wheat seedlings. Treatments at 1.0, 2.0, and 3.0 mg Se kg(-1) significantly increased root activity, proline content, peroxidase (POD), and catalase (CAT) activities, carotenoids (Car) content, chlorophyll content, and reduced malondialdehyde (MDA) content of wheat seedlings. Lower Se treatment did not significantly effect on chlorophyll content and MDA content, although it also increased some antioxidant index (proline and Car content, POD and CAT activities) in wheat seedlings. These results suggest that optimal Se supply is favorable for growth of wheat seedlings during drought condition.

Topics: Biomass; Carotenoids; Catalase; Chlorophyll; Coloring Agents; Dehydration; Lipid Peroxidation; Lipid Peroxides; Peroxidase; Plant Leaves; Plant Proteins; Plant Roots; Proline; Seedlings; Selenium; Sodium Selenite; Tetrazolium Salts; Trace Elements; Triticum

Peanut, found to be relatively drought tolerant crop, has been the choice of study to characterize the genes expressed under gradual water deficit stress. Nearly 700 genes were identified to be enriched in subtractive cDNA library from gradual process of drought stress adaptation. Further, expression of the drought inducible genes related to various signaling components and gene sets involved in protecting cellular function has been described based on dot blot experiments. Fifty genes (25 regulators and 25 functional related genes) selected based on dot blot experiments were tested for their stress responsiveness using northern blot analysis and confirmed their nature of differential regulation under different field capacity of drought stress treatments. ESTs generated from this subtracted cDNA library offered a rich source of stress-related genes including signaling components. Additional 50% uncharacterized sequences are noteworthy. Insights gained from this study would provide the foundation for further studies to understand the question of how peanut plants are able to adapt to naturally occurring harsh drought conditions. At present functional validation cannot be deemed in peanut, hence as a proof of concept seven orthologues of drought induced genes of peanut have been silenced in heterologous N. benthamiana system, using virus induced gene silencing method. These results point out the functional importance for HSP70 gene and key regulators such as Jumonji in drought stress response.

Topics: Arachis; Base Sequence; Chlorophyll; Dehydration; DNA; DNA, Complementary; Droughts; Expressed Sequence Tags; Gene Library; Gene Silencing; Genes, Plant; HSP70 Heat-Shock Proteins; Molecular Sequence Data; Nicotiana; Plant Leaves

The gender of dimorphic plant species is often affected by ecophysiological variables. Differences have been interpreted as a response of the sexes to meet specific resource demands associated with reproduction. This study investigated whether sex-specific variations in ecophysiological traits in response to water availability determine the performance of each sex in different habitats, and therefore promote extreme spatial segregation of the sexes in the subdioecious plant, Honckenya peploides. Twenty-seven plants of each sex were individually potted in dune sand and assigned randomly to one of three water treatments. Well-watered plants were watered daily to field capacity, whereas plants in the moderate and high-water stress treatments received 40% and 20%, respectively, of the water given to well-watered plants. Photochemical efficiency, leaf spectral properties and components of relative growth rate (leaf area ratio and net assimilation rate) were measured. Photochemical efficiencies integrated over time were higher in male than in female plants. Water deficit decreased maximum quantum yield in female plants more rapidly than in male plants, but female plants (unlike male plants) had recovered to initial values by the end of the experiment. Maximum quantum yield in male plants was more affected by water stress than in female plants, indicating that male plants were more susceptible to photoinhibition. The two sexes did not differ in growth rate, but male plants invested a higher proportion of their biomass in leaves, had a higher leaf area per unit biomass and lower net assimilation rate relative to female plants. Female plants had a higher water content and succulence than male plants. Differences in stomatal density between the sexes depended on water availability. The results suggest that the two sexes of H. peploides have different strategies for coping with water stress. The study also provides evidence of sex differences in allocation traits. We conclude that between-sex differences in ecophysiological and allocation traits may contribute to explain habitat-related between-sex differences in performance and, therefore, the spatial segregation of the sexes.

Topics: Adaptation, Physiological; Caryophyllaceae; Chlorophyll; Dehydration; Fluorescence; Light; Photosynthesis; Plant Leaves; Time Factors; Water

Kinetic studies of protein dephosphorylation in barley thylakoid membranes revealed accelerated dephosphorylation of photosystem II (PSII) proteins, and meanwhile rapidly induced phosphorylation of a light-harvesting complex (LHCII) b4, CP29 under water stress. Inhibition of dephosphorylation aggravates stress damages and hampers photosystem recovery after rewatering. This increased dephosphorylation is catalyzed by both intrinsic and extrinsic membrane protein phosphatase. Water stress did not cause any thylakoid destacking, and the lateral migration from granum membranes to stroma-exposed lamellae was only found to CP29, but not other PSII proteins. Activation of plastid proteases and release of TLP40, an inhibitor of the membrane phosphatases, were also enhanced during water stress. Phosphorylation of CP29 may facilitate disassociation of LHCII from PSII complex, disassembly of the LHCII trimer and its subsequent degradation, while general dephosphorylation of PSII proteins may be involved in repair cycle of PSII proteins and stress-response-signaling.

Topics: Chlorophyll; Dehydration; Enzyme Activation; Hordeum; Immunophilins; Intracellular Membranes; Light-Harvesting Protein Complexes; Peptide Hydrolases; Phosphoric Monoester Hydrolases; Phosphorylation; Photosynthesis; Photosystem II Protein Complex; Plant Proteins; Seedlings; Thylakoids

This study investigated oxidative stress and the antioxidant response to boron (B) of chickpea cultivars differing in their tolerance to drought. Three-week-old chickpea seedlings were subjected to 0.05 (control), 1.6 or 6.4 mm B in the form of boric acid (H(3)BO(3)) for 7 days. At the end of the treatment period, shoot length, dry weight, chlorophyll fluorescence, B concentration, malondialdehyte content and the antioxidant enzymes superoxide dismutase (SOD), peroxidase (POX), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) were measured. The 1.6 mm B treatment did not cause significant changes in shoot length of cultivars, although shoot length increased in the drought-tolerant Gökce and decreased in the drought-sensitive Küsmen after 6.4 mm B treatment. Dry weights of both cultivars decreased with 6.4 mm B treatment. Chlorophyll fluorescence (Fv/Fm) did not change in Gökce at either B level. Nor did it change in Küsmen with 1.6 mm B but Fv/Fm decreased with 6.4 mm B. Boron concentration in the shoots of both cultivars increased significantly with increasing levels of applied B. Significant increases in total SOD activity were observed in shoots of both cultivars given 1.6 and 6.4 mm B. Shoot extracts exhibited five activity bands, two of which were identified as MnSOD and Cu/ZnSOD. In comparison to the control group, all enzyme activities (except APX and SOD) decreased with 1.6 mm B stress. GR activity decreased, while activities of CAT, POX and APX did not change with 6.4 mm B in Küsmen. On the other hand, activities of CAT, APX and SOD increased in Gökce at both B levels. In addition, lipid peroxidation was higher in Küsmen than in Gökce, indicating more damage by B to membrane lipids in the former cultivar. These results suggest that (i) Gökce is tolerant and Küsmen is sensitive to B, and (ii) B tolerance of Gökce might be closely related to increased capacity of the antioxidative system (total SOD, CAT and APX) to scavenge reactive oxygen species and thus suppress lipid peroxidation under B stress. To the best of our knowledge, this is the first report on the antioxidant response of chickpea seedlings to B toxicity.

Topics: Antioxidants; Ascorbate Peroxidases; Biological Transport; Boric Acids; Boron; Catalase; Chlorophyll; Cicer; Dehydration; Glutathione Reductase; Lipid Peroxidation; Oxidative Stress; Peroxidase; Peroxidases; Photosynthesis; Plant Proteins; Plant Shoots; Superoxide Dismutase

Cuttings of Populus przewalskii and P. cathayana, which originated from high and low altitudes in southwest China, were used to examine the effect of water stress on the morphological, physiological and biochemical traits of plants in a greenhouse for one growing season. The dry mass accumulation and allocation, gas exchanges, extent of peroxidation damage, osmotic adjustment and antioxidative defenses, and amounts of pigments were measured to characterize the differences in peroxidation damage and protective mechanisms of two poplar species that contrast in drought tolerance. Under water stress, poplars showed a series of biochemical adjustments and morphological changes as follows: a decrease in leaf relative water content, gas exchanges, plant growth and dry mass accumulation; an increase in relative allocation to roots; an increase in the osmolyte contents (e.g. total amino acids). Additionally, water deficit induced an increase in peroxidation damage [as indicated by an increase in electrolyte leakage, malondialdehyde (MDA), carbonyl (C = O ) and hydrogen peroxide (H(2) O(2) ) content], enhanced activities or contents of antioxidants (e.g. ascorbate peroxidase, guaiacol peroxidase, glutathione redutase and ascorbic acid) and reduced amounts of leaf pigments (e.g. chlorophyll and carotenoid). Furthermore, there were significant differences in the extent of morphological and biochemical changes between the two poplar species. Compared with P. cathayana, P. przewalskii responded to water stress by allocating relatively more to root dry mass, possessing a higher net photosynthesis rate, and having more efficient protective mechanisms, such as more osmolyte accumulation, stronger antioxidant activities and lower chlorophyll/carotenoid ratio. Thus, P. przewalskii suffered less damage as deduced from lower levels of electrolyte leakage, MDA, C=O and H(2) O(2) content. Therefore, P. przewalskii originating from high altitude could possess more efficient protective mechanisms than P. cathayana, which is from low-altitude habitats.

Topics: Altitude; Amino Acids; Ascorbate Peroxidases; Ascorbic Acid; Biomass; Carbohydrates; Carotenoids; China; Chlorophyll; Dehydration; Ecosystem; Electrolytes; Gases; Glutathione Peroxidase; Hydrogen Peroxide; Malondialdehyde; Peroxidase; Plant Leaves; Populus; Solubility; Species Specificity; Water

Measurement of stable isotopes in plant dry matter is a useful phenotypic tool for speeding up breeding advance in C(3) crops exposed to different water regimes. However, the situation in C(4) crops is far from resolved, since their photosynthetic metabolism precludes (at least in maize) the use of carbon isotope discrimination. This paper investigates the use of oxygen isotope enrichment (Delta(18)O) as a new secondary trait for yield potential and drought resistance in maize (Zea mays L). A set of tropical maize hybrids developed by the International Maize and Wheat Improvement Center was grown under three contrasting water regimes in field conditions. Water regimes clearly affected plant growth and yield. In accordance with the current theory, a decrease in water input was translated into large decreases in stomatal conductance and increases in leaf temperature together with concomitant (18)O enrichment of plant matter (leaves and kernels). In addition, kernel Delta(18)O correlated negatively with grain yield under well-watered and intermediate water stress conditions, while it correlated positively under severe water stress conditions. Therefore, genotypes showing lower kernel Delta(18)O under well-watered and intermediate water stress had higher yields in these environments, while the opposite trend was found under severe water stress conditions. This illustrates the usefulness of Delta(18)O for selecting the genotypes best suited to differing water conditions.

Topics: Adaptation, Physiological; Chlorophyll; Dehydration; Droughts; Fluorescence; Genotype; Oxygen; Oxygen Isotopes; Photosynthesis; Plant Leaves; Plant Stomata; Quantitative Trait, Heritable; Temperature; Water; Zea mays

Drought stress has multiple effects on the photosynthetic system. Here, we show that a decrease of the relative contribution of the I-P phase, DeltaV(IP) = -V(I) = (F(M)-F(I))/(F(M)- F(o)), to the fluorescence transient OJIP is observed in 10 drought-stressed barley and 9 chickpea varieties. The extent of the I-P loss in the barley varieties depended on their drought tolerance. The relative loss of the I-P phase seems to be related to a loss of photosystem (PS) I reaction centers as determined by 820-nm transmission measurements. In the second part of this study, the interaction of drought and heat stress in two barley varieties (the drought tolerant variety Aït Baha and the drought sensitive variety Lannaceur) was studied using a new approach. Heat stress was induced by exposing the plant leaves to temperatures of 25-45 degrees C and the inactivation of the O(2)-evolving complex (OEC) was followed measuring chlorophyll a (Chl a) fluorescence using a protocol consisting of two 5-ms pulses spaced 2.3 ms apart. In active reaction centers, the dark interval is long enough to allow the OEC to recover from the first pulse; whereas in heat-inactivated reaction centers it is not. In the latter category of reaction centers, no further fluorescence rise is induced by the second pulse. Lannaceur, under well-watered conditions, was more heat tolerant than Aït Baha. However, this difference was lost following drought stress. Drought stress considerably increased the thermostability of PS II of both varieties.

Topics: Chlorophyll; Chlorophyll A; Dehydration; Fluorescence; Hordeum; Hot Temperature; Photosystem I Protein Complex; Photosystem II Protein Complex

In order to explore the relevant molecular genetic mechanisms of photosynthetic rate (PR) and chlorophyll content (CC) in rice (Oryza sativa L.), we conducted a series of related experiments using a population of recombinant inbred lines (Zhenshan97B x IRAT109). We found a significant correlation between CC and PR (R= 0.19**) in well-watered conditions, but no significant correlation during water stress (r= 0.08). We detected 13 main quantitative trait loci (QTLs) located on chromosomes 1, 2, 3, 4, 5, 6, and 10, which were associated with CC, including six QTLs located on chromosomes 1, 2, 3, 4, and 5 during water stress, and seven QTLs located on chromosomes 2, 3, 4, 6, and 10 in well-watered conditions. These QTLs explained 47.39% of phenotypic variation during water stress and 56.19% in well-watered conditions. We detected four main QTLs associated with PR; three of them (qPR2, qPR10, qPR11) were located on chromosomes 2, 10, and 11 during water stress, and one (qPR10) was located on chromosome 10 in well-watered conditions. These QTLs explained 34.37% and 18.41% of the phenotypic variation in water stress and well-watered conditions, respectively. In total, CC was largely controlled by main QTLs, and PR was mainly controlled by epistatic QTL pairs.

Topics: Chlorophyll; Dehydration; Gene Expression Regulation, Plant; Oryza; Photosynthesis; Quantitative Trait Loci

The mechanisms involved in desiccation tolerance of lichens and their photobionts are still poorly understood. To better understand these mechanisms we have studied dehydration rate and desiccation time in Trebouxia, the most abundant chlorophytic photobiont in lichen. Our findings indicate that the drying rate has a profound effect on the recovery of photosynthetic activity of algae after rehydration, greater than the effects of desiccation duration. The basal fluorescence (F'(o)) values in desiccated algae were significantly higher after rapid dehydration, than after slow dehydration, suggesting higher levels of light energy dissipation in slow-dried algae. Higher values of PSII electron transport were recovered after rehydration of slow-dried Trebouxia erici compared to rapid-dried algae. The main component of non-photochemical quenching after slow dehydration was energy dependent (q (E)), whereas after fast dehydration it was photoinhibition (q (I)). Although q (E) seems to play a role during desiccation recovery, no significant variations were detected in the xanthophyll cycle components. Desiccation did not affect PSI functionality. Classical antioxidant activities like superoxide dismutase or peroxidase decreased during desiccation and early recovery. Dehydrins were detected in the lichen-forming algae T. erici and were constitutively expressed. There is probably a minimal period required to develop strategies which will facilitate transition to the desiccated state in this algae. In this process, the xanthophyll cycle and classical antioxidant mechanisms play a very limited role, if any. However, our results indicate that there is an alternative mechanism of light energy dissipation during desiccation, where activation is dependent on a sufficiently slow dehydration rate.

Topics: Antioxidants; Blotting, Western; Carotenoids; Cell Membrane; Chlorophyll; Dehydration; Desiccation; Electrolytes; Electrophoresis, Polyacrylamide Gel; Fluorescence; Kinetics; Lichens; Light; Models, Biological; Oxidation-Reduction; Photosystem I Protein Complex; Plant Proteins; Time Factors; Xanthophylls

The effects on morphological and physiological characters of durum wheat (Triticum durum Desf.) plants issued from seeds upon irradiation with low doses of cobalt gamma rays (i.e., 0, 10, 20 and 30 Gy), were studied. The study is carried out in the Experimental Research Station of Ecole Supérieure d'Agriculture du Kef (North West Tunisia) in 2008/2009. In Petri dishes, the 20 Gy dose caused an increase of the speed and Germination Capacity (GC) of the seeds as compared to non irradiated ones. Plants from these treated seeds maintained on Knops' culture medium (culture medium used to study plant growth in test tubes), improved root system in terms of length, volume and weight when compared to the plants issued from the non treated seeds. This irradiation dose (20 Gy) also improved in a significant way the above ground system growth of the plants. Under glass house conditions with a water stress, the plants issued from seeds treated with 20 Gy, had higher water content and membrane stability as compared of those from the non irradiated ones. Furthermore, seed irradiation with this dose had a positive effect on the chlorophyll content and maximum quantum yield of the irradiated plants. These results suggest that ionizing irradiation may be considered as an alternative in improving root growth of the plant and therefore controlling drought.

Topics: Cell Membrane; Chlorophyll; Dehydration; Gamma Rays; Germination; Photosynthesis; Plant Leaves; Plant Roots; Seeds; Triticum; Water

Phytohormones play critical roles in regulating plant responses to stress. We investigated the effects of water stress induced by adding 12% (w/v) polyethylene glycol to the root medium on the levels of abscisic acid (ABA), indole-3-acid (IAA), zeatin (ZT), and gibberellin(3) (GA(3)) in maize leaves. The results suggested that water stress had significant effects on the four hormone levels. There was a transient increase in the IAA content during the initial stage of adaptation to water stress in maize leaves, but it dropped sharply thereafter in response to water stress. ABA content increased dramatically in maize leaves after 24 h of exposure to water stress, and then the high levels of ABA were maintained to the end. The contents of ZT and GA(3) rapidly declined in maize leaves subjected to water stress. The effects of water stress on chlorophyll content, electrolyte leakage and malondialdehyde levels in maize leaves were also studied. The variation of cell damage was negatively correlated with ZT and GA(3) levels in maize leaves under water stress. Thus, we explored the roles of ZT and GA(3) on the growth of maize seedlings under water stress by exogenous application. It is possible that both ZT and GA(3) were effective in protecting maize seedlings from water stress, which would be of great importance for the improvement of drought tolerance in maize by genetic manipulation.

Topics: Chlorophyll; Dehydration; Electrolytes; Gibberellins; Malondialdehyde; Plant Growth Regulators; Plant Leaves; Seedlings; Water; Zea mays; Zeatin

To study the effect of water stress on the growth and accumulation of active components of Isatis indigotica.. By pot culture experiment,the characteristic indexes of water requirement, chlorophyll content, transpiration rate, photosynthesis, biomass and effective ingredient indirubin were studied under normal and water stress conditions, respectively.. With the increase of the extent and duration of water stress, the quantity of water requirement, chlorophyll content, respiration rate, photosynthesis and biomass of I. indigotica were decreased, while water use efficiency increased.. It is a critical period of water requirement in July, high water content in soil and extreme water stress are not propitious to the accumulation of indirubin, appropriate water stress can stimulate indirubin accumulatio. The harvest of leaves of I. indigotica is optimal in July, while the harvest of root is appropriate in October. Yield and quantity of I. indigotica are improved when the field capacity is between 45% and 70%.

Topics: Biomass; Cell Respiration; Chlorophyll; Dehydration; Indoles; Isatis; Photosynthesis; Plant Leaves; Plant Roots; Plants, Medicinal

In the present investigation, two watering treatments, viz., 100% and 60% field capacity (FC) were used to understand the effects of water deficit on early growth, biomass allocation, pigment and biochemical constituents and proline metabolism of five varieties of Sunflower (Helianthus annuus L.) plants. We found that there was a significant difference in early growth, dry matter accumulation, pigment, biochemical constituents and proline metabolism among the five varieties. The root length, shoot length, total leaf area, fresh and dry weight, chlorophyll a, b, total chlorophyll and carotenoid were significantly reduced under water stress treatments. Water stress increased the proline, free amino acid and glycinebetaine contents along with increased activity of gamma-glutamyl kinase but the activity of proline oxidase reduced as a consequence of water stress.

Topics: Betaine; Carotenoids; Chlorophyll; Dehydration; Disasters; Helianthus; Proline; Random Allocation

While increasing temperatures and altered soil moisture arising from climate change in the next 50 years are projected to decrease yield of food crops, elevated CO2 concentration ([CO2]) is predicted to enhance yield and offset these detrimental factors. However, C4 photosynthesis is usually saturated at current [CO2] and theoretically should not be stimulated under elevated [CO2]. Nevertheless, some controlled environment studies have reported direct stimulation of C4 photosynthesis and productivity, as well as physiological acclimation, under elevated [CO2]. To test if these effects occur in the open air and within the Corn Belt, maize (Zea mays) was grown in ambient [CO2] (376 micromol mol(-1)) and elevated [CO2] (550 micromol mol(-1)) using Free-Air Concentration Enrichment technology. The 2004 season had ideal growing conditions in which the crop did not experience water stress. In the absence of water stress, growth at elevated [CO2] did not stimulate photosynthesis, biomass, or yield. Nor was there any CO2 effect on the activity of key photosynthetic enzymes, or metabolic markers of carbon and nitrogen status. Stomatal conductance was lower (-34%) and soil moisture was higher (up to 31%), consistent with reduced crop water use. The results provide unique field evidence that photosynthesis and production of maize may be unaffected by rising [CO2] in the absence of drought. This suggests that rising [CO2] may not provide the full dividend to North American maize production anticipated in projections of future global food supply.

Topics: Agriculture; Amino Acids; Biomass; Carbohydrate Metabolism; Carbon Dioxide; Chlorophyll; Dehydration; Forecasting; Greenhouse Effect; Light; Nitrogen; Photosynthesis; Plant Leaves; Soil; Water; Zea mays

Protein composition and native state of chlorophylls were analyzed in two wheat (Triticum durum L.) genotypes with different tolerance to drought, Barakatli-95 (drought-tolerant) and Garagylchyg-2 (drought-sensitive), during water deficit. It is shown that the plants subjected to water deficit appear to have a slight increase in alpha- and beta-subunits of CF1 ATP-synthase complex (57.5 and 55 kD, respectively) in Barakatli-95 and their lower content in Garagylchyg-2. Steady-state levels of the core antenna of PS II (CP47 and CP43) and light-harvesting Chl a/b-apoproteins (LHC) II in the 29.5-24 kD region remained more or less unchanged in both wheat genotypes. The synthesis of 36 kD protein and content of low-molecular-weight polypeptides (21.5, 16.5, and 14 kD) were noticeably increased in the tolerant genotype Barakatli-95. Drought caused significant changes in the carotenoid region of the spectrum (400-500 nm) in drought-sensitive genotype Garagylchyg-2 (especially in the content of pigments of the violaxanthin cycle). A shift of the main band from 740-742 to 738 nm is observed in the fluorescence spectra (77 K) of chloroplasts from both genotypes under water deficiency, and there is a stimulation of the ratio of fluorescence band intensity F687/F740.

Topics: Adaptation, Biological; Chlorophyll; Chlorophyll A; Dehydration; Electrophoresis, Polyacrylamide Gel; Genotype; Light-Harvesting Protein Complexes; Photosynthetic Reaction Center Complex Proteins; Photosystem I Protein Complex; Photosystem II Protein Complex; Plant Proteins; Rosaniline Dyes; Spectrometry, Fluorescence; Spectrophotometry; Thylakoids; Triticum

The ability of partial dehydration and abscisic acid pretreatments to increase desiccation tolerance in the cyanobacterial lichen Peltigera polydactylon was tested. *. Net photosynthesis and respiration were measured using infrared gas analysis during a drying and rehydration cycle. At the same time, the efficiency of photosystem two was measured using chlorophyll fluorescence, and the concentrations of chlorophyll a were spectrophotometrically assayed. Heat production was also measured during a shorter drying and rehydration cycle using differential dark microcalorimetry. *. Pretreating lichens by dehydrating them to a relative water content of approx. 0.65 for 3 d, followed by storing thalli hydrated for 1 d in the light, significantly improved their ability to recover net photosynthesis during rehydration after desiccation for 15 but not 30 d. Abscisic acid pretreatment could substitute for partial dehydration. The improved rates of photosynthesis during the rehydration of pretreated material were not accompanied by preservation of photosystem two activity or chlorophyll a concentrations compared with untreated lichens. Partial dehydration and ABA pretreatments appeared to have little direct effect on the desiccation tolerance of the mycobiont, because the bursts of respiration and heat production that occurred during rehydration were similar in control and pretreated lichens. *. Results indicate that the photobiont of P. polydactylon possesses inducible tolerance mechanisms that reduce desiccation-induced damage to carbon fixation, and will therefore improve the supply of carbohydrates to the whole thallus following stress. In this lichen, ABA is involved in signal transduction pathways that increase tolerance of the photobiont.

Topics: Abscisic Acid; Chlorophyll; Chlorophyll A; Dehydration; Hot Temperature; Lichens; Oxidative Stress; Oxygen Consumption; Photosynthesis; Photosystem II Protein Complex; Time Factors; Water

Water stress is by far the leading environmental stress limiting crop yields worldwide. Genetic engineering techniques hold great promise for developing crop cultivars with high tolerance to water stress. In this study, the Brassica oleracea var. acephala BoRS1 gene was transferred into tobacco through Agrobacterium-mediated leaf disc transformation. The transgenic status and transgene expression of the transgenic plants was confirmed by polymerase chain reaction (PCR) analysis, Southern hybridization and semi-quantitative one step RT-PCR analysis respectively. Subsequently, the growth status under water stress, and physiological responses to water stress of transgenic tobacco were studied. The results showed that the transgenic plants exhibited better growth status under water stress condition compared to the untransformed control plants. In physiological assessment of water tolerance, transgenic plants showed more dry matter accumulation and maintained significantly higher levels of leaf chlorophyll content along with increasing levels of water stress than the untransformed control plants. This study shows that BoRS1 is a candidate gene in the engineering of crops for enhanced water stress tolerance.

Topics: Agrobacterium tumefaciens; Biological Assay; Blotting, Southern; Brassica; Chlorophyll; Dehydration; Germination; Heat-Shock Proteins; Nicotiana; Plant Proteins; Plants, Genetically Modified; Plasmids; Polymerase Chain Reaction; Transformation, Genetic

The influence of water deprivation on potted apple trees (Malus domestica Borkh.) was investigated. Biochemical responses including ascorbic acid, glutathione (GSH), tocopherols, chlorophylls, carotenoids, free amino acids, and soluble carbohydrates were measured in leaves of diploid 'Elstar' and triploid 'Jonagold Wilmuta' subjected to progressive drought. Leaf water potential was chosen to be the primary indicator of water stress in tested plants. Time courses of measured biochemical parameters showed that mild drought did not significantly affect the chosen stress indicators. Moderate drought increased the concentrations of ascorbic acid, total GSH, beta-carotene, zeaxanthin and alpha-tocopherol, indicating the adaptation to oxidative stress in apple trees. Moderate drought also increased concentration of soluble carbohydrates, mostly due to increased sorbitol concentration. Severe drought negatively affected vitality of apple trees, and caused a decrease in sorbitol concentration. Severe drought also caused decreases in ascorbic acid, total GSH, beta-carotene, alpha-tocopherol and chlorophyll concentrations, which, together with the increase in oxidised GSH concentration, indicated severe damage due to oxidative stress. Severe drought increased free amino acid concentration, which was probably the result of increased proteolysis. Zeaxanthin concentration remained high even in leaves of apple trees subjected to severe drought stress. The results were similar for both tested apple cultivars.

Topics: Adaptation, Physiological; Amino Acids; Antioxidants; Ascorbic Acid; Carbohydrate Metabolism; Carotenoids; Chlorophyll; Dehydration; Diploidy; Malus; Plant Leaves; Polyploidy; Tocopherols; Water

To assess genotypic variation in drought response of silver birch (Betula pendula Roth), we studied the plasticity of 16 physiological traits in response to a 12-14-week summer drought imposed on four clones in two consecutive years. In a common garden experiment, 1-year-old clonal trees from regions with low (550 mm year(-1)) to high rainfall (1270 mm year(-1)) were grown in 45-l pots, and leaf gas exchange parameters, leaf water potentials, leaf osmotic potentials and leaf carbon isotope signatures were repeatedly measured. There were no clonal differences in leaf water potential, but stomatal conductance (gs), net photosynthesis at ambient carbon dioxide concentration, photosynthetic water-use efficiency, leaf carbon isotope composition (delta13C) and leaf osmotic potentials at saturation (Pi0) and at incipient plasmolysis (Pip) were markedly influenced by genotype, especially gs and osmotic adjustment. Genotypes of low-rainfall origin displayed larger osmotic adjustment than genotypes of high-rainfall origin, although their Pi0 and Pip values were similar or higher with ample water supply. Genotypes of low-rainfall origin had higher gs than genotypes of high-rainfall origin under both ample and limited water supply, indicating a higher water consumption that might increase competitiveness in drought-prone habitats. Although most parameters tested were significantly influenced by genotype and treatment, the genotype x treatment interactions were not significant. The genotypes differed in plasticity of the tested parameters and in their apparent adaptation to drought; however, among genotypes, physiological plasticity and drought adaptation were not related to each other. Reduction of gs was the first and most plastic response to drought in all genotypes, and allowed the maintenance of high predawn leaf water potentials during the drought. None of the clones exhibited non-stomatal limitation of photosynthesis. Leaf gs, photosynthetic capacity, magnitude of osmotic adjustment and delta13C were all markedly lower in 2000 than in 1999, indicating root limitation in the containers in the second year.

Topics: Betula; Carbon; Chlorophyll; Dehydration; Genetic Variation; Phenotype; Photosynthesis; Plant Leaves; Trees

Physiological responses to water stress in hybrid poplar (INRA 7171-B4, Populus tremula L. x P. alba L.) lines transformed to overexpress a pine cytosolic glutamine synthetase (GS1) gene were compared with those of non-transgenic plants. Before, during and after a drought treatment, net photosynthetic rates (Anet) were higher in transgenic than in non-transgenic plants. Stomatal conductance (gs) was higher in transgenic than in non-transgenic plants before, but not after exposure to drought. Before drought treatment, a sudden reduction in photosynthetic photon flux caused a greater burst of CO2 efflux in transgenic than non-transgenic plants, indicating greater photorespiratory activity. Drought caused greater reductions in photochemical quenching, photosystem II (PSII) antennae transfer efficiency (Fv'/Fm') and light-adapted PSII yield (PhiPSII) in non-transgenic than in transgenic plants, especially at low irradiances. Antennae-based thermal dissipation was higher in transgenic plants than in non-transgenic plants both during the imposition of drought and 1 or 3 days after the relief of drought. Under severe water stress and subsequently, transgenic plants maintained a higher expression of glutamine synthetase, glutamate synthase and Rubisco and higher concentrations of chlorophyll and glycine than non-transgenic plants. These findings indicate that overexpression of pine cytosolic GS1 enhanced sustained photosynthetic electron transport capacity during severe stomatal limitation. The data also suggest that ectopic expression of cytosolic GS increases photorespiratory activity, and that this serves as a protective sink for electrons from photosynthetic reaction centers.

Topics: Chlorophyll; Dehydration; Gene Expression Regulation, Enzymologic; Glutamate-Ammonia Ligase; Photosynthesis; Plant Transpiration; Plants, Genetically Modified; Populus; Trees

Holm oak (Quercus ilex L.) is native to hot, dry Mediterranean forests where limited water availability often reduces photosynthesis in many species, and forest fires are frequent. Holm oaks resprout after a disturbance, with improved photosynthetic activity and water relations compared with unburned plants. To better understand the role of water availability in this improvement, watering was withheld from container-grown plants, either intact (controls) or resprouts after excision of the shoot, to gradually obtain a wide range of soil water availabilities. At high water availability, gas exchange rates did not differ between controls and resprouts. At moderate soil dryness, net photosynthesis of control plants decreased as a result of increased stomatal limitation, whereas gas exchange rates of resprouts, which had higher midday and predawn leaf water potentials, were unchanged. Under severe drought, resprouts showed a less marked decline in gas exchange than controls and maintained photosystem II integrity, as indicated by chlorophyll fluorescence measurements. Photosynthesis was down-regulated in both plant types in response to reduced CO2 availability caused by high stomatal limitation. Lower non-stomatal limitations in resprouts than in control plants, as evidenced by higher carboxylation velocity and the capacity for ribulose-1,5-bisphosphate regeneration, conferred greater drought resistance under external constraints similar to summer conditions at midday.

Topics: Carbon Dioxide; Chlorophyll; Dehydration; Down-Regulation; Photosynthesis; Plant Leaves; Plant Transpiration; Quercus; Soil; Trees; Water

Drought is a major abiotic stress that limits plant growth and crop productivity. A spontaneous wilty mutant (eibi1) hypersensitive to drought was identified from wild barley (Hordeum spontaneum Koch). eibi1 showed the highest relative water loss rate among the known wilty mutants, which indicates that eibi1 is one of the most drought-sensitive mutants. eibi1 had the same abscisic acid (ABA) level, the same ability to accumulate stress-induced ABA, and the same stomatal movement in response to light, dark, drought, and exogenous ABA as the wild type, revealing that eibi1 was neither an ABA-deficient nor an ABA-insensitive mutant. The eibi1 leaves had a larger chlorophyll efflux rate in 80% ethanol than the wild-type leaves; and the transpiration rate of eibi1 was more closely related to chlorophyll efflux rate than to stomatal density, demonstrating that the cuticle of eibi1 was defective. eibi1 will be a promising candidate to study the actual barrier layer in the cuticle that limits water loss of the plant. Exogenous ABA reduced leaf length growth in eibi1 more than in the wild type, implying an interaction on plant growth of ABA signal transduction and the eibi1 product. One may infer that the eibi1 product may reverse the growth inhibition induced by ABA.

Topics: Abscisic Acid; Chlorophyll; Crosses, Genetic; Dehydration; Genes, Plant; Germination; Hordeum; Mutation; Plant Leaves; Plant Transpiration

Growth, development and productivity of long-lived organisms such as forest trees are continuously challenged by abiotic stresses, and may also be greatly affected by predicted climatic change. As a first step toward creating stress-resistant maritime pine (Pinus pinaster Ait.) varieties by marker-assisted breeding, we describe the identification and characterization of water-stress-responsive genes in hydroponically grown seedlings that were well watered (-0.08 MPa) or subjected to water deprivation (-0.45 MPa) by the addition of polyethylene glycol. The cDNA amplified fragment-length polymorphism (cDNA-AFLP) technique was used to identify genes regulated by water deprivation. Approximately 4000 transcript-derived fragments (TDFs) were screened, of which 28 increased and 20 decreased in seedlings subjected to water deprivation. Of these 48 TDFs, 62.6% corresponded to proteins of known function, which indicate the main mechanisms involved in the osmotic stress response (photosynthesis, carbohydrate metabolism, cell wall synthesis and plant defense). We found that 16.6% of the 48 TDFs were similar to Arabidopsis thaliana (L.) Heynh gene products, 10.4% were similar to Pinus taeda L. expressed sequence tags (ESTs) and 10.4% did not match any sequences in the public databases. The relative abundance of these transcripts was quantitatively analyzed by reverse northern of both needle and root tissues, confirming the effectiveness of the cDNA-AFLP technique in detecting differentially expressed genes. The identification and characterization of water-stress-responsive genes provide new insights into the nature of the machinery involved in the response to water deprivation in a forest tree.

Topics: Blotting, Northern; Chlorophyll; Dehydration; DNA Fingerprinting; Gene Expression Regulation, Plant; Genes, Plant; Hydroponics; Pinus; Plant Leaves; Plant Roots; Sequence Homology, Nucleic Acid; Trees

We used bulked segregant analysis (BSA) to identify microsatellite markers associated with water-stress tolerance in wheat. Two DNA pools (tolerant and sensitive) were established from the selected F2 individuals of crosses between water-stress-tolerant and -sensitive wheat parental genotypes on the basis of the paraquat (PQ) tolerance, leaf size, and relative water content. All three traits were previously shown to be associated with water-stress tolerance on segregating F2 progeny of the wheat crosses used in this study. Microsatellite analysis was then performed on the established DNA pools, using 35 primer pairs that included all of the chromosome group 5 (5A, 5B, 5D) markers, to detect microsatellite fragments that were present, absent, or both in the DNA pools and their parental lines. We identified one microsatellite fragment that was present in tolerant parent wheat and the tolerant bulk but absent in the sensitive parent wheat and sensitive bulk. We then followed the segregation of this marker in the tolerant F2 individuals. Use of this marker may significantly enhance the success of selection for PQ- and water-stress-tolerant genotypes in wheat breeding programs.

Topics: Base Sequence; Breeding; Chlorophyll; Dehydration; DNA Fingerprinting; Gene Expression Profiling; Gene Expression Regulation, Plant; Genetic Markers; Microsatellite Repeats; Molecular Sequence Data; Paraquat; Plant Leaves; Polymorphism, Genetic; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Triticum; Water

We compared responses to drought and re-watering of greenhouse-grown cuttings of Populus x euramericana (Dode) Guinier clones, Luisa Avanzo and Dorskamp. Total leaf area, leaf number, leaf area increment and stomatal conductance were evaluated periodically during a 29-day drought period and for 16 days after re-watering. Soil water content and predawn leaf water potential (Psi(wp)) were measured on Days 29 and 45. On the same days, relative water content (RWC), specific leaf area (SLA), nitrogen, chlorophyll, soluble sugars, total phenols, flavanols and antioxidant activity were determined for leaves taken from the bottom to the top of each cutting. Leaves of Luisa Avanzo cuttings grew more rapidly than leaves of Dorskamp and exhibited higher SLA, but lower concentrations of nitrogen, chlorophyll and soluble sugars and lower antioxidant activity per unit area. On Day 29, after withholding water, both clones had closed their stomata, reduced rates of leaf growth, and lower Psi(wp) and RWC; however, the clones differed in their responses to soil water depletion. Compared to Dorskamp, Luisa Avanzo closed its stomata earlier and maintained higher Psi(wp), but lower RWC and leaf sugar concentrations. Antioxidant activity of leaf methanolic extracts decreased in response to water stress only in Luisa Avanzo. Leaf physiology and its modulation by water stress were age dependent in Luisa Avanzo.

Topics: Antioxidants; Carbohydrates; Chlorophyll; Dehydration; Flavonoids; Flavonols; Nitrogen; Phenols; Plant Leaves; Plant Transpiration; Populus; Trees; Water

The upper elevational limits of tree species are thought to be controlled by abiotic factors such as temperature and the soil and atmospheric conditions affecting plant water status. We measured relative water contents (RWC), water potentials (Psi) and cuticular conductances (g(c)) of shoots of four conifer species-eastern hemlock (Tsuga canadensis (L.) Carr.), eastern white pine (Pinus strobus L.), red pine (P. resinosa Ait.) and red spruce (Picea rubens Sarg.)-during two winters on Mt. Ascutney, Vermont, USA. Some micrometeorological measurements are also reported. Eastern hemlock and white pine were studied near their upper elevational limits at a 640-m site, and red pine was studied near its upper elevational limit at 715 m. Red spruce was also studied at the 715-m site, which is in the middle of its elevational range on this mountain. There was no evidence of winter desiccation stress in any species. The observed distribution of seedlings suggested that the upper elevational limits on shade-intolerant eastern white pine and red pine are set by the absence of suitable seed beds after 100 years without fire. Eastern hemlock is able to reproduce in deep shade on organic substrates, but germination at high elevations may be restricted by low temperatures.

Topics: Altitude; Chlorophyll; Dehydration; Picea; Pinaceae; Pinus; Plant Leaves; Plant Transpiration; Seasons; Temperature; Trees; Tsuga; Water

Apart from red (chlorophyll a) fluorescence, ultraviolet (UV) excitation of plant leaves results in blue-green intrinsic light emission. In order to analyse the potentially modifying influence of stress on this blue-green fluorescence (BGF), and to determine its origin, the fluorescence was characterised by spectroscopy, and two and three dimensional microscopy in leaves of Hordeum vulgare L. under UV and drought stress. Leaves of wild-type and proanthocyanidin-free mutant barley were compared. In general, the BGF was enhanced by stress. The analysis of the distribution of BGF pointed to differences in response to the different stress conditions and served to indicate possible sources of the fluorescence. This study showed that (1) the selective enhancement of BGF by supplementary UV radiation (280-360 nm) in upper leaf tissues of wild-type but not in proanthocyanidin-free leaves, pointed to flavonoids as possible emitters; (2) drought-inducible BGF emission was found in cell walls and to a smaller extent in upper leaf tissue regardless of genotype, thus independent of impairment in flavonoid biosynthesis and (3) intracellular BGF, which co-localised with red chlorophyll fluorescence, was inducible in mesophyll cells of both genotypes by short-term application of high levels of UV radiation.

Topics: Anthocyanins; Chlorophyll; Dehydration; Fluorescence; Hordeum; Microscopy, Confocal; Plant Leaves; Proanthocyanidins; Spectrometry, Fluorescence; Time Factors; Ultraviolet Rays

Myrothamnus flabellifolia, a short woody shrub from southern Africa, can survive severe desiccation of its vegetative organs. We studied mechanisms protecting this plant from oxidative damage during desiccation for 2 weeks, 4 and 8 months, and also during subsequent rehydration. This plant retains high concentrations of chlorophyll during desiccation, and these chlorophyll molecules are probably a source for potentially harmful singlet oxygen production. Desiccation triggered substantial increases in zeaxanthin and redox shifts of the antioxidants glutathione and ascorbate towards their oxidised forms. Simultaneously, the concentrations of violaxanthin, beta-carotene, ascorbate, alpha-tocopherol, and glutathione reductase activity progressively decreased. Antheraxanthin, gamma-tocopherol, lutein, neoxanthin and glucose-6-phosphate dehydrogenase displayed less pronounced changes in response to desiccation. Even after 4 months of desiccation, Myrothamnus flabellifolia recovered rapidly upon rehydration. Re-watering induced formation of ascorbate and glutathione, simultaneous reduction of their oxidised forms, and rapid production of alpha-tocopherol and of various carotenoids. Only after 8 months of desiccation did the antioxidant system of M. flabellifolia break down; 3 weeks after the onset of rehydration, these plants abscised their leaves, but even then they were still able to recover and develop new ones. Ascorbate, beta-carotene and alpha-tocopherol were totally depleted after 8 months of desiccation and did not recover upon rehydration; glutathione was partly maintained, but only in the oxidised form. We present a model demonstrating which parts of antioxidant pathways break down as oxidative stress becomes detrimental and we discuss some potential implications of our results for the genetic modification of crop plants to improve their drought tolerance.

Topics: Antioxidants; Ascorbic Acid; beta Carotene; Chlorophyll; Dehydration; Glutathione; Magnoliopsida; Oxidative Stress; Plant Diseases; Tocopherols; Xanthophylls

Green leafy vegetables are the most underexploited class of vegetables despite high nutritional value. Reports on nutritional composition and storage of some of the fresh leaves are available but the storage behavior of dehydrated leaves apparently has not been studied. Therefore, in the present study, two green leafy vegetables, savoy beets (Beta vulgaris var. bengalensis) and fenugreek (Trigonella foenum graecum) were dehydrated in a low temperature drier and stored for 9 months under ambient and cold stored conditions after packaging in single or double layers of high density polyethylene film (200 gauge). The quality was determined on the basis of retention of beta-carotene, ascorbic acid and chlorophyll, and the extent of browning during storage. Retention of these quality characteristics depended on the crop and storage conditions. Double packed and cold stored samples of fenugreek retained 67% beta-carotene, whereas savoy beet leaves retained only 57% of the initial beta-carotene under similar conditions. Similarly, higher retention of ascorbic acid and chlorophyll, and lower browning was observed in double packed, cold stored samples. Results indicated the efficacy of double packed and cold stored samples over other combinations.

Topics: Ascorbic Acid; beta Carotene; Chlorophyll; Color; Dehydration; Food Handling; Food Packaging; Food Preservation; Humans; Nutritive Value; Plant Leaves; Taste; Temperature; Time Factors; Trigonella; Vegetables