chloramine-t and Inflammation

Apolipoprotein A-I (apoA-I) is the major lipoprotein component of high-density lipoprotein(HDL), and plays an important role in reverse cholesterol transport. Its function is known to be influenced by oxidation.. Using H2O2-or chloramine T-oxidized apoA-I as antigen, we prepared 2 kinds of monoclonal antibodies, and established an ELISA system for the measurement of oxidized apoA-I.. The 2 monoclonal antibodies obtained, 7D3 and 98A7, exhibited different reactivity characteristics. The serum level of oxidized apoA-I was higher in patients with either inflammatory disease or diabetes than in healthy individuals, and suggested a diversity of oxidized apoA-I.. The 2 monoclonal antibodies are useful for the determination of oxidized apoA-I and study of diverse oxidized HDLs.

Topics: Adult; Aged; Antibodies, Monoclonal; Apolipoprotein A-I; Chloramines; Chromatography, Affinity; Diabetes Mellitus; Enzyme-Linked Immunosorbent Assay; Female; Humans; Inflammation; Lipoproteins, HDL; Male; Middle Aged; Oxidation-Reduction; Tosyl Compounds

The effects of two commonly used antiseptics for chronic ulcer care, chloramine T and chlorhexidine, on the biochemical and histological parameters of healing have been examined. The antiseptics were compared with saline when applied to 10 mm skin defects on Sprague-Dawley rats. Chloramine T significantly delayed the production of collagen and prolonged the acute inflammatory response compared with saline. Chlorhexidine did not differ from saline. We conclude that hypochlorite antiseptics may adversely affect healing by secondary intention and the antibacterial and desloughing action of agents should be carefully balanced against their toxic effect.

Topics: Animals; Anti-Infective Agents, Local; Chloramines; Chlorhexidine; Collagen; Female; Fibroblasts; Inflammation; Rats; Rats, Inbred Strains; Tosyl Compounds; Wound Healing