chloramine-t and Diabetes-Mellitus

Apolipoprotein A-I (apoA-I) is the major lipoprotein component of high-density lipoprotein(HDL), and plays an important role in reverse cholesterol transport. Its function is known to be influenced by oxidation.. Using H2O2-or chloramine T-oxidized apoA-I as antigen, we prepared 2 kinds of monoclonal antibodies, and established an ELISA system for the measurement of oxidized apoA-I.. The 2 monoclonal antibodies obtained, 7D3 and 98A7, exhibited different reactivity characteristics. The serum level of oxidized apoA-I was higher in patients with either inflammatory disease or diabetes than in healthy individuals, and suggested a diversity of oxidized apoA-I.. The 2 monoclonal antibodies are useful for the determination of oxidized apoA-I and study of diverse oxidized HDLs.

Topics: Adult; Aged; Antibodies, Monoclonal; Apolipoprotein A-I; Chloramines; Chromatography, Affinity; Diabetes Mellitus; Enzyme-Linked Immunosorbent Assay; Female; Humans; Inflammation; Lipoproteins, HDL; Male; Middle Aged; Oxidation-Reduction; Tosyl Compounds

A radioimmunoassay was developed for the detection of 7 S collagen, a basement membrane component derived from type IV collagen, in sera of patients. Two forms of 7 S collagen were isolated by limited collagenase digestion of type IV collagen from bovine placenta. Antisera against both forms were raised in rabbits. The antigens were labeled by conjugation with the Bolton Hunter reagent or by the Chloramine T method. Free and bound antigen were separated using a second antibody directed against rabbit-IgG. Serum concentrations of 7 S collagen ranged from 5-14 micrograms/l. Serum levels of 7 S collagen were increased in diabetic patients as compared to normal subjects.

Topics: Adolescent; Adult; Aged; Chloramines; Collagen; Diabetes Mellitus; Humans; Middle Aged; Molecular Weight; Radioimmunoassay; Succinimides; Tosyl Compounds