chiniofon and Colitis

Roquinimex is a modulator of the immune system and has been shown to attenuate induction of several inflammatory and autoimmune diseases. The objective of the present study was to determine the efficacy of roquinimex in a model of murine colitis.. For this purpose, Balb/c mice were exposed to 5% dextran sodium sulfate (DSS) in the drinking water for five to six days. Roquinimex (300 mg kg(-1) day(-1)) was administered by subcutaneous (s.c.) injection 3 days prior to and throughout the treatment period with DSS. In separate experiments, 300 mg kg(-1) day(-1) of roquinimex was given therapeutically after initiation of DSS challenge.. DSS provoked clinical signs of colitis, reduced crypt height (CH) and increased mucosal damage score (MDS) as analyzed by histology. In addition, challenge with DSS increased the colonic content of myeloperoxidase (MPO). Prophylactic administration of DSS-treated mice with roquinimex significantly reduced clinical signs of colitis, MDS and the CH-reduction. Moreover, in roquinimex treated animals, the MPO activity was significantly reduced by more than 50% compared to DSS control mice. Notably, therapeutic administration of roquinimex in DSS-treated mice also significantly inhibited the MDS, CH-reduction and MPO activity.. These findings suggest that roquinimex strongly inhibits murine colitis and may provide a novel pharmacological approach to treat inflammatory bowel disease.

Topics: Adjuvants, Immunologic; Animals; Colitis; Colon; Dextran Sulfate; Hydroxyquinolines; Injections, Subcutaneous; Male; Mice; Mice, Inbred BALB C; Peroxidase

Topics: Colitis; Female; Humans; Hydroxyquinolines; Indium; Middle Aged; Neutrophils; Organometallic Compounds; Oxyquinoline; Radionuclide Imaging

The accuracy and usefulness of indium-111 oxine-labeled autologous leukocyte scans in the management of infectious complications of colon and rectal surgery and of inflammatory diseases of the colon have been studied by review of the records of all patients undergoing such scans at Morristown Memorial Hospital during the first six months such scans were performed there. A total of 20 scans was performed on 18 patients. Twelve scans were performed on 11 patients being treated for diseases of the colon and rectum. Of these 12 scans, one was normal, four demonstrated intra-abdominal abscesses, three demonstrated wound infections (two abdominal, one perineal), one was positive for granulomatous colitis, one for pseudomembranous colitis, one for chronic diverticulitis, and one for enterocutaneous fistula. One allergic reaction to the labeled leukocytes occurred. Comparisons with other corroborative localizing diagnostic tests were made. Subsequent treatment was reviewed. Analysis of these cases indicates that the majority of all scans were performed to evaluate complications of colorectal surgery or inflammatory diseases of the colon and that these scans were highly accurate. In all instances where appropriate, delineation of a lesion in the colorectal cases was followed by corrective treatment.

Topics: Abdomen; Abscess; Colitis; Colonic Diseases; Humans; Hydroxyquinolines; Indium; Leukocytes; Organometallic Compounds; Oxyquinoline; Radioisotopes; Radionuclide Imaging; Rectal Diseases; Surgical Wound Dehiscence; Surgical Wound Infection

Topics: Adult; Chronic Disease; Colitis; Drug Resistance, Microbial; Enteritis; Enterobacteriaceae; Female; Fungi; Humans; Hydroxyquinolines; Intestine, Small; Male; Microbial Sensitivity Tests; Middle Aged; Nitrofurans; Staphylococcus; Streptococcus

Topics: Adult; Biological Products; Colitis; Enteritis; Escherichia coli; Female; Humans; Hydroxyquinolines; Intestinal Mucosa; Intestine, Small; Male; Middle Aged