chebulagic-acid and Enterovirus-Infections

chebulagic-acid has been researched along with Enterovirus-Infections* in 2 studies

Other Studies

2 other study(ies) available for chebulagic-acid and Enterovirus-Infections

Article	Year
Small molecules targeting coxsackievirus A16 capsid inactivate viral particles and prevent viral binding. Emerging microbes & infections, 2018, Sep-26, Volume: 7, Issue:1 Coxsackievirus A16 (CVA16) is an etiologic agent of hand, foot, and mouth disease (HFMD) that affects young children, and although typically self-limited, severe complications, and fatal cases have been reported. Due to the lack of specific medication and vaccines against CVA16, there is currently a need to develop effective antivirals to better control CVA16 infections in epidemic areas. In this study, we identified the tannins chebulagic acid (CHLA) and punicalagin (PUG) as small molecules that can efficiently disrupt the CVA16 infection of human rhabdomyosarcoma cells. Both compounds significantly reduced CVA16 infectivity at micromolar concentrations without apparent cytotoxicity. A mechanistic analysis revealed that the tannins particularly targeted the CVA16 entry phase by inactivating cell-free viral particles and inhibiting viral binding. Further examination by molecular docking analysis pinpointed the targets of the tannins in the fivefold axis canyon region of the CVA16 capsid near the pocket entrance that functions in cell surface receptor binding. We suggest that CHLA and PUG are efficient antagonists of CVA16 entry and could be of value as antiviral candidates or as starting points for developing molecules to treat CVA16 infections. Topics: Antiviral Agents; Benzopyrans; Capsid Proteins; Enterovirus A, Human; Enterovirus Infections; Glucosides; Humans; Hydrolyzable Tannins; Molecular Docking Simulation; Small Molecule Libraries; Tannins; Virus Attachment	2018
Chebulagic acid, a hydrolyzable tannin, exhibited antiviral activity in vitro and in vivo against human enterovirus 71. International journal of molecular sciences, 2013, May-03, Volume: 14, Issue:5 Human enterovirus 71 is one of the major causative agents of hand, foot and mouth disease in children under six years of age. Presently, no vaccines or antiviral drugs have been clinically available to employ against EV71. In this study, we demonstrate that treatment with chebulagic acid reduced the viral cytopathic effect on rhabdomyosarcoma cells with an IC50 of 12.5 μg/mL. The utilization of the chebulagic acid treatment on mice challenged with a lethal dose of enterovirus 71 was able to efficiently reduce mortality and relieve clinical symptoms through the inhibition of viral replication. Chebulagic acid may represent a potential therapeutic agent to control infections to enterovirus 71. Topics: Animals; Antiviral Agents; Benzopyrans; Cell Line; Enterovirus A, Human; Enterovirus Infections; Glucosides; Humans; Hydrolyzable Tannins; Mice; Virus Replication	2013

Article

Year

Small molecules targeting coxsackievirus A16 capsid inactivate viral particles and prevent viral binding.

Emerging microbes & infections, 2018, Sep-26, Volume: 7, Issue:1

Coxsackievirus A16 (CVA16) is an etiologic agent of hand, foot, and mouth disease (HFMD) that affects young children, and although typically self-limited, severe complications, and fatal cases have been reported. Due to the lack of specific medication and vaccines against CVA16, there is currently a need to develop effective antivirals to better control CVA16 infections in epidemic areas. In this study, we identified the tannins chebulagic acid (CHLA) and punicalagin (PUG) as small molecules that can efficiently disrupt the CVA16 infection of human rhabdomyosarcoma cells. Both compounds significantly reduced CVA16 infectivity at micromolar concentrations without apparent cytotoxicity. A mechanistic analysis revealed that the tannins particularly targeted the CVA16 entry phase by inactivating cell-free viral particles and inhibiting viral binding. Further examination by molecular docking analysis pinpointed the targets of the tannins in the fivefold axis canyon region of the CVA16 capsid near the pocket entrance that functions in cell surface receptor binding. We suggest that CHLA and PUG are efficient antagonists of CVA16 entry and could be of value as antiviral candidates or as starting points for developing molecules to treat CVA16 infections.

Topics: Antiviral Agents; Benzopyrans; Capsid Proteins; Enterovirus A, Human; Enterovirus Infections; Glucosides; Humans; Hydrolyzable Tannins; Molecular Docking Simulation; Small Molecule Libraries; Tannins; Virus Attachment

2018

Chebulagic acid, a hydrolyzable tannin, exhibited antiviral activity in vitro and in vivo against human enterovirus 71.

International journal of molecular sciences, 2013, May-03, Volume: 14, Issue:5

Human enterovirus 71 is one of the major causative agents of hand, foot and mouth disease in children under six years of age. Presently, no vaccines or antiviral drugs have been clinically available to employ against EV71. In this study, we demonstrate that treatment with chebulagic acid reduced the viral cytopathic effect on rhabdomyosarcoma cells with an IC50 of 12.5 μg/mL. The utilization of the chebulagic acid treatment on mice challenged with a lethal dose of enterovirus 71 was able to efficiently reduce mortality and relieve clinical symptoms through the inhibition of viral replication. Chebulagic acid may represent a potential therapeutic agent to control infections to enterovirus 71.

Topics: Animals; Antiviral Agents; Benzopyrans; Cell Line; Enterovirus A, Human; Enterovirus Infections; Glucosides; Humans; Hydrolyzable Tannins; Mice; Virus Replication

2013