cgs-27023a and Colitis

cgs-27023a has been researched along with Colitis* in 1 studies

Other Studies

1 other study(ies) available for cgs-27023a and Colitis

ArticleYear
Matrix metalloproteinase-9 modulates intestinal injury in rats with transmural colitis.
    Journal of leukocyte biology, 2006, Volume: 79, Issue:5

    Proteolysis and degradation of extracellular matrix by metalloproteinases (MMPs) may contribute to intestinal injury in inflammatory bowel disease. In the present study, we investigated the pathogenic role of gelatinases (MMP-9 and MMP-2) on transmural colonic injury in a rat model of chronic colitis, which was induced by intracolonic instillation of trinitrobenzene sulfonic acid (TNBS). The activity and expression of MMP-2 and MMP-9 were measured in colonic tissue and peripheral neutrophils by fluorescence, zymography, Western blot, or immunohistochemistry at different time-points. Furthermore, myeloperoxidase content in colonic homogenates was analyzed to evaluate inflammation. Finally, morphological changes were assessed following early or delayed administration of CGS-27023-A, a synthetic inhibitor of MMPs. We found that the induction of colitis led to a significant up-regulation in tissue gelatinase concentration, whereas no changes in collagenase activity were observed. In addition, up-regulation of pro-MMP-9, but not of pro-MMP-2, was found on Days 7 and 10 following the induction of colitis. Furthermore, transmural MMP-9 was detected by immunofluorescent staining in the inflamed tissue. Consistent with tissue samples, neutrophils from colitic rats showed a significantly increased activity of pro-MMP-9. Finally, early but not delayed treatment with CGS-27023-A attenuated colonic mucosal injury in rats with TNBS-induced colitis. In conclusion, up-regulation of MMP-9 in peripheral and colonic neutrophils modulates transmural colonic injury in rats with TNBS-induced colitis.

    Topics: Animals; Colitis; Colon; Disease Models, Animal; Enzyme Activation; Enzyme Inhibitors; Hydroxamic Acids; Inflammation Mediators; Intestinal Mucosa; Male; Matrix Metalloproteinase 2; Matrix Metalloproteinase 9; Matrix Metalloproteinase Inhibitors; Neutrophils; Pyrazines; Rats; Rats, Sprague-Dawley; Sulfonamides; Trinitrobenzenesulfonic Acid; Up-Regulation

2006