ceruletide and Stomach-Ulcer

Topics: Animals; Cats; Ceruletide; Cholecystokinin; Digestive System; Digestive System Physiological Phenomena; Dogs; Duodenal Ulcer; Gastric Juice; Gastrins; Gastrointestinal Diseases; Gastrointestinal Hormones; Gastrointestinal Motility; Glucagon; Guinea Pigs; Humans; Pancreas; Secretin; Stomach Ulcer

Recent studies have shown that stimulation of cannabinoid 1 (CB1) receptor reduces the area of ischemic myocardial necrosis and affects activity of the digestive tract. The aim of the present study was to check whether the administration of CB1 receptor agonist or antagonist affects the stress-induced gastric ulceration and development of edematous pancreatitis.. Experiments were performed on rats. Gastric lesions were induced by water immersion and restrain stress (WRS). Acute pancreatitis was induced by cerulein. Prior to WRS or before and during cerulein administration, a natural endogenous ligand for CB1 receptor, anandamide was administered intraperitoneally at the dose of 0.8, 1.5 or 3.0 micromol/kg. A synthetic CB1 receptor antagonist, AM 251 (ALEXIS(R) Biochemicals) was administrated at the dose of 4 micromol/kg i.p. alone or in combination with anandamide at the dose of 1.5 micromol/kg.. Administration of anandamide reduced gastric lesions and this effect was associated with am increase in gastric mucosal blood flow and mucosal DNA synthesis; whereas serum level of pro-inflammatory interleukin-1 beta was reduced. Treatment with AM 251 aggravated gastric damage and reversed protective effect of anandamide administration. Opposite effect was observed in the pancreas. Administration of anandamide increased dose-dependently the severity of pancreatitis. In histological examination, we observed an increase in pancreatic edema and inflammatory infiltration. Also, treatment with anandamide augmented the pancreatitis-induced increase in serum level of lipase, amylase, poly-C ribonuclease, and pro-inflammatory interleukin-1 beta; whereas pancreatic DNA synthesis was reduced. Treatment with AM 251 reduced histological and biochemical signs of pancreatic damage and reversed deleterious effect of anandamide in cerulein-induced acute pancreatitis.. Activation of CB1 receptors evokes opposite effects in the stomach and pancreas: in the stomach, exhibits protective effect against stress-induced gastric mucosal lesions; whereas in the pancreas, increases the severity of cerulein-induced pancreatitis.

Topics: Acute Disease; Animals; Arachidonic Acids; Cannabinoids; Ceruletide; DNA; Endocannabinoids; Gastric Mucosa; Interleukin-1beta; Male; Pancreas; Pancreatitis; Piperidines; Polyunsaturated Alkamides; Pyrazoles; Rats; Rats, Wistar; Receptor, Cannabinoid, CB1; Restraint, Physical; Stomach Ulcer; Stress, Physiological

The effect of caerulein injected into cerebroventricle on the stress-induced gastric mucosal lesion of rats with tied four limbs immersed in water was investigated. Caerulein (1.0 ng/rat) reduced significantly gastric mucosal lesion, decreased gastric acid content, but increased the gastric content of the mucus and PGE2. Under electromicroscope, morphological sign of hyposecretion in the parietal cells and hypersecretion in the mucus cells could be seen. The effect of caerulein could be prevented by intraventricular injection of naloxone or subcutaneous injection of indomethacin, but not by atropine, phentolamine and propranolol. The results indicate that the protective effect of caerulein on the gastric mucosa is mediated partly by central morphine receptors and partly by enhancement of endogenous PGE2.

Topics: Animals; Ceruletide; Dinoprostone; Female; Gastric Juice; Gastric Mucosa; Injections, Intraventricular; Male; Rats; Rats, Wistar; Receptors, Opioid; Stomach Ulcer; Stress, Physiological

In the present paper the gastric secretory and motor responsiveness to a gastrin-like peptide, caerulein, was assessed in rats with a chronic gastric ulcer induced by 'isolation', 48 h after completing prolonged treatments (30 and 60 days) with cimetidine (80 and 160 mg/kg), pirenzepine (8 and 16 mg/kg) and sulglycotide (160 mg/kg) administered orally as a single daily dose. After a 30 day pretreatment with both doses of cimetidine, gastric acid secretion was inhibited and the pylorus spasmogenic activity induced by caerulein was enhanced. The gastric effects of the peptide were not modified by pirenzepine pretreatment while an antisecretory action was shown by sulglycotide after the completion of prolonged treatment (60 days). The ulcers were significantly reduced by cimetidine (low dose) and sulglycotide after 30 day pretreatment. The effects are more likely to be related to the treatment than to the presence of the drugs on gastric receptors.

Topics: Animals; Anti-Ulcer Agents; Ceruletide; Chronic Disease; Female; Gastric Acid; Gastric Mucosa; Gastrointestinal Motility; Pepsin A; Rats; Rats, Inbred Strains; Stomach Ulcer

The stimulating effect of AOC-tetragastrin, caerulein, Histalog and secretin on human gastric acid and pepsin secretion was studied in gastric ulcer patients. The pattern of gastric acid and pepsin secretion after the administration of caerulein was closely resembled to that of gastrin. Slight increase of pepsin secretion after gastrin or caerulein could be based on "wash-out" action caused by the increase of acid secretion after the stimulants. Stimulating effect on gastric pepsin secretion of histalog and secretin would be independent of gastric acid secretion.

Topics: Ceruletide; Gastric Juice; Gastrins; Humans; Pepsin A; Secretin; Stomach Ulcer