ceruletide and Fistula

The effects on pancreatic secretion of a diet supplemented with wheat bran for one month were studied in five dogs provided with chronic pancreatic and gastric fistulae. Dogs were subjected before and after bran administration to a continuous intravenous infusion of secretin (GIH, 0.5 U/kg/h) alone and a combination of secretin with cerulein (25 or 100 ng/kg/h). Exocrine pancreatic secretions under basal conditions were collected during the intravenous infusions of normal saline. Wheat bran supplementation for 4 wk to the standard diet in dogs increased pancreatic juice flow rate along with bicarbonate and amylase output whether the secretion was unstimulated or stimulated with caerulein alone or combined with secretin. No significant changes in the concentration of pancreatic bicarbonate, amylase, chymotrypsin, and proteins were noted under stimulated conditions. Furthermore, lipase concentration and output were dramatically reduced. These data indicated that wheat bran supplemented to the standard diet in dogs affects the exocrine pancreatic secretion whether it was unstimulated or stimulated by secretin alone or combined with cerulein.

Topics: Animals; Ceruletide; Dietary Fiber; Dogs; Fistula; Gastric Fistula; Pancreas; Pancreatic Juice; Secretin

In dogs with chronic pancreatic fistulas, pure cholecystokinin (CCK), CCK-variant, 20% pure CCK, octapeptide of CCK, and caerulein all gave the same high maximal protein outputs and low maximal bicarbonate outputs. 10% pure CCK gave higher bicarbonate output that can be achieved with pure CCK. By intestinal perfusion, HCI gave greater protein output than can be achieved with secretine alone, sodium oleate gave greater bicarbonate output than can be achieved with pure CCK alone, and tryptophan gave maximal bicarbonate output less than that of pure CCK. On the hypothesis that intestinally perfused stimulants act solely by release of secretin and CCK, the effects of tryptophan can be accounted for by release of CCK alone, of HCI by release mainly of secretin plus small amounts of CCK, and of oleate by release mainly of CCK plus small amounts of secretin.

Topics: Animals; Ceruletide; Cholecystokinin; Dogs; Dose-Response Relationship, Drug; Fistula; Hydrochloric Acid; Oleic Acids; Pancreas; Peptides; Proteins; Secretin; Stimulation, Chemical; Stomach; Swine; Tryptophan

Topics: Animals; Bicarbonates; Ceruletide; Cholecystokinin; Dogs; Drug Synergism; Fistula; Gastrointestinal Hormones; Kinetics; Leucine; Pancreas; Peptides; Proteins; Secretin; Stomach; Tryptophan; Vagotomy; Vagus Nerve