cellulose and Trypanosomiasis

cellulose has been researched along with Trypanosomiasis* in 2 studies

Other Studies

2 other study(ies) available for cellulose and Trypanosomiasis

Article	Year
Cryopreservation of Trypanosoma evansi after DEAE-cellulose purification: Evaluation of infective parameters. Research in veterinary science, 2011, Volume: 90, Issue:2 Cryopreservation is a method of keeping parasites alive in a laboratory. However, this technique may also damage the parasite. Alternatively, parasites may be maintained by in vitro culture. Unfortunately, for Trypanosoma evansi no effective medium that is able to maintain the parasite for more than 4 months has been described. In this study, we examined the effect of purifying trypomastigote through DEAE-cellulose chromatography before and after cryopreservation, by analyzing the pre-patent period, longevity, parasitemia, and count of viable parasites. Our results showed a three-times increase in the concentration of viable trypomastigote in DEAE-purified cryopreserved parasites as compared to non-DEAE-purified cryopreserved parasites. This indicates that DEAE-cellulose chromatography followed by cryopreservation is an effective method for the storage and preservation of T. evansi, with the advantage that the stocked parasites will be ready to use in molecular biology procedures. Topics: Animals; Anion Exchange Resins; Chromatography; Cryopreservation; DEAE-Cellulose; Female; Rats; Rats, Wistar; Trypanosoma; Trypanosomiasis	2011
Use of the miniature anion exchange centrifugation technique to isolate Trypanosoma evansi from goats. Annals of the New York Academy of Sciences, 2004, Volume: 1026 DEAE (anion exchanger diethylaminoethyl)-cellulose and mini Anion Exchange Centrifugation Technique (mAECT) allow salivarian trypanosomes to be separated from the blood of affected animals. The purpose of this study was to assess the mAECT in goats infected with T. evansi. Five adult Canary goats were inoculated intravenously with at least 1 x 10(5) T. evansi isolated from a dromedary camel in the Canary Islands. The goats were monitored for specific antibodies and parasite detection. The inoculated goats became infected and the parasitemia remained very low but was persistent. For mAECT columns, the DEAE gel was equilibrated with phosphate-buffered saline glucose. T. evansi was detected by its mobility with a microscope at low magnification (10 x 10). The mAECT proved to be more sensitive than blood smear and buffy coat but less sensitive than mouse inoculation. We conclude that in cases of very low parasitemia in goats, mAECT can be used when other parasite-detection tests have failed. Topics: Animals; Anion Exchange Resins; Centrifugation; DEAE-Cellulose; Female; Goat Diseases; Goats; Sensitivity and Specificity; Trypanosoma; Trypanosomiasis	2004

Article

Year

Cryopreservation of Trypanosoma evansi after DEAE-cellulose purification: Evaluation of infective parameters.

Research in veterinary science, 2011, Volume: 90, Issue:2

Cryopreservation is a method of keeping parasites alive in a laboratory. However, this technique may also damage the parasite. Alternatively, parasites may be maintained by in vitro culture. Unfortunately, for Trypanosoma evansi no effective medium that is able to maintain the parasite for more than 4 months has been described. In this study, we examined the effect of purifying trypomastigote through DEAE-cellulose chromatography before and after cryopreservation, by analyzing the pre-patent period, longevity, parasitemia, and count of viable parasites. Our results showed a three-times increase in the concentration of viable trypomastigote in DEAE-purified cryopreserved parasites as compared to non-DEAE-purified cryopreserved parasites. This indicates that DEAE-cellulose chromatography followed by cryopreservation is an effective method for the storage and preservation of T. evansi, with the advantage that the stocked parasites will be ready to use in molecular biology procedures.

Topics: Animals; Anion Exchange Resins; Chromatography; Cryopreservation; DEAE-Cellulose; Female; Rats; Rats, Wistar; Trypanosoma; Trypanosomiasis

2011

Use of the miniature anion exchange centrifugation technique to isolate Trypanosoma evansi from goats.

Annals of the New York Academy of Sciences, 2004, Volume: 1026

DEAE (anion exchanger diethylaminoethyl)-cellulose and mini Anion Exchange Centrifugation Technique (mAECT) allow salivarian trypanosomes to be separated from the blood of affected animals. The purpose of this study was to assess the mAECT in goats infected with T. evansi. Five adult Canary goats were inoculated intravenously with at least 1 x 10(5) T. evansi isolated from a dromedary camel in the Canary Islands. The goats were monitored for specific antibodies and parasite detection. The inoculated goats became infected and the parasitemia remained very low but was persistent. For mAECT columns, the DEAE gel was equilibrated with phosphate-buffered saline glucose. T. evansi was detected by its mobility with a microscope at low magnification (10 x 10). The mAECT proved to be more sensitive than blood smear and buffy coat but less sensitive than mouse inoculation. We conclude that in cases of very low parasitemia in goats, mAECT can be used when other parasite-detection tests have failed.

Topics: Animals; Anion Exchange Resins; Centrifugation; DEAE-Cellulose; Female; Goat Diseases; Goats; Sensitivity and Specificity; Trypanosoma; Trypanosomiasis

2004