cellulose and Neoplasms

Topics: Acute Disease; Animals; Cell Line; DEAE-Cellulose; DNA; Durapatite; Glucocorticoids; Humans; Hydroxyapatites; Kinetics; Neoplasms; Rats; Receptors, Glucocorticoid; Receptors, Steroid

Rapid (6 min washing step) and reliable methods for determining uridine kinase and pyrimidine phosphoribosyltransferase activities were devised. These procedures, consisting of a spotting technique on DEAE-discs followed by washing and elution, permitted the consistent recovery of about 90% of the nucleoside 5'-phosphate esters formed from radioactive precursors, either uridine or 5-fluorouracil, respectively. Of these precursors, less than 2% were retained on the discs. Direct counting of the discs (without elution), filtration by either gravity or suction, and the use of so-called activation of discs have not proved advantageous.

Topics: Adsorption; Cells, Cultured; Cellulose; DEAE-Cellulose; Humans; Methods; Neoplasms; Pentosyltransferases; Phosphotransferases; Pyrimidines; Uridine Kinase

Topics: Adsorption; Clinical Enzyme Tests; Communicable Diseases; DEAE-Cellulose; Diagnosis, Differential; Hepatitis; Hepatitis A; Humans; Hydroxybutyrate Dehydrogenase; Hypertension; L-Lactate Dehydrogenase; Myocardial Infarction; Neoplasms; Uremia

Topics: Catalase; Chromatography; DEAE-Cellulose; Endotoxins; Neoplasms