cellulose and Leukodystrophy--Metachromatic

cellulose has been researched along with Leukodystrophy--Metachromatic* in 1 studies

Other Studies

1 other study(ies) available for cellulose and Leukodystrophy--Metachromatic

ArticleYear
Direct tandem mass spectrometric profiling of sulfatides in dry urinary samples for screening of metachromatic leukodystrophy.
    Clinica chimica acta; international journal of clinical chemistry, 2013, Oct-21, Volume: 425

    Prediagnostic steps in suspected metachromatic leukodystrophy (MLD) rely on clinical chemical methods other than enzyme assays. We report a new diagnostic method which evaluates changes in the spectrum of molecular types of sulfatides (3-O-sulfogalactosyl ceramides) in MLD urine.. The procedure allows isolation of urinary sulfatides by solid-phase extraction on DEAE-cellulose membranes, transportation of a dry membrane followed by elution and tandem mass spectrometry (MS/MS) analysis in the clinical laboratory. Major sulfatide isoforms are normalized to the least variable component of the spectrum, which is the indigenous C18:0 isoform. This procedure does not require the use of specific internal standards and minimizes errors caused by sample preparation and measurement.. Urinary sulfatides were analyzed in a set of 21 samples from patients affected by sulfatidosis. The combined abundance of the five most elevated isoforms, C22:0, C22:0-OH, C24:0, C24:1-OH, and C24:0-OH sulfatides, was found to give the greatest distinction between MLD-affected patients and a control group.. The method avoids transportation of liquid urine samples and generates stable membrane-bound sulfatide samples that can be stored at ambient temperature. MS/MS sulfatide profiling targeted on the most MLD-representative isoforms is simple with robust results and is suitable for screening.

    Topics: Adolescent; Case-Control Studies; Child; Child, Preschool; DEAE-Cellulose; Desiccation; Female; Humans; Infant; Leukodystrophy, Metachromatic; Male; Membranes, Artificial; Middle Aged; Reference Standards; Solid Phase Extraction; Specimen Handling; Sulfoglycosphingolipids; Tandem Mass Spectrometry

2013