cellulase and Melanoma
cellulase has been researched along with Melanoma* in 1 studies
Other Studies
1 other study(ies) available for cellulase and Melanoma
Article | Year |
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Enzymatically Extracted Apple Pectin Possesses Antioxidant and Antitumor Activity.
The biological activity of apple pectin extracted conventionally or enzymatically using endo-xylanase and endo-cellulase, was tested in vitro. The analyses were performerd in tetraplicates and the statistical significance of the differences were assessed using ANOVA, Tukey post hoc and LSD (the least significant difference) tests. Multivariate regression analysis was applied to determine the structural components that have a crucial importance for antioxidant and antitumor properties of pectins. The pectins extracted by enzymes contained up to four times more ferulic acid and showed twice as great ability to neutralize free radicals and Fe(III) reduction. The antiradical potential positively correlated with phenols, fucose and rhamnose content. In the assays performed on HT-29 human adenocarcinoma and B16F10 melanoma cell cultures, the "green" pectins, contrary to acid isolated ones, exhibited remarkable anti-neoplastic potential while being nontoxic to nontransformed L929 cell line. The pectins in the dose of 1 mg/mL were capable of inhibiting adhesion (max 23.1%), proliferation (max 40.4%), invasion (max 76.9%) and anchorage-independent growth (max 90%) of HT-29 cells (significance level Topics: Antineoplastic Agents; Antioxidants; Apoptosis; Cell Proliferation; Cellulase; Colonic Neoplasms; Endo-1,4-beta Xylanases; Humans; Malus; Melanoma; Pectins; Tumor Cells, Cultured | 2021 |