cefoxitin and Anemia--Refractory--with-Excess-of-Blasts

In order to examine expression of the Tn antigen on erythroid cells from a patient with Tn syndrome, we applied a selective two phase liquid culture system for human erythroid progenitors in peripheral blood. The cells were analyzed with flow cytometry employing an anti-Tn antibody and a lectin of Vicia villosa which recognizes only the Tn determinant. In the second phase, the Tn antigen was expressed on the cultured cells from the patient on day 3 and Tn-positive cells reached 62.7% on day 9. On the other hand, Tn-positive cells were not detected in the volunteer's cultured cells. When the patient's cells were co-cultured with the cells from a healthy volunteer, the percentage of Tn-positive cells was much lower than the expected value, suggesting that the normal cells suppressed the expression of Tn antigen on the patient's cells.

Topics: Acetylgalactosamine; Anemia, Refractory, with Excess of Blasts; Antigens, Surface; Antigens, Tumor-Associated, Carbohydrate; Cell Differentiation; Erythrocyte Aggregation; Erythrocytes, Abnormal; Erythroid Precursor Cells; Flow Cytometry; Galactosyltransferases; Hemagglutination Tests; Hematologic Diseases; Humans; Male; Membrane Glycoproteins; Middle Aged; Syndrome