cefotaxime and Tuberculosis

Tuberculosis (TB) caused 1.8 million deaths worldwide with increased multiple drug resistance (MDR) cases estimated 4.8 lakhs in the year 2015. β-Lactam antibiotics could be a hope for TB treatment. Therefore, in this study, uniformity in the biochemical and molecular nature of β-lactamases was analyzed to evaluate the potential of β-lactam antibiotics as a treatment regimen against Mycobacterium tuberculosis (MTB).. β-Lactamase enzymes in 233 MTB clinical isolates along with control H37Rv strain were characterized by enzyme kinetic using nitrocefin and cefotaxime as a substrate, isoelectric points by isoelectric focusing electrophoresis (IEF) and by PCR and Southern blotting.. This confirmed that MTB β-lactamase enzymes belong to the Class A, group 2be Extended Spectrum β-Lactamases with no biochemical or molecular polymorphism. ESBLs are mainly responsible for resistance against β-lactam antibiotics in MTB. Thus ESBLs could be the potential therapeutic target for TB treatment using β-lactam antibiotics in combination with β-lactamase inhibitors like sulbactam and sodium clavulanate.

Topics: Anti-Bacterial Agents; beta-Lactam Resistance; beta-Lactamases; beta-Lactams; Blotting, Southern; Cefotaxime; Cephalosporins; Clavulanic Acid; Humans; Isoelectric Focusing; Kinetics; Mycobacterium tuberculosis; Polymerase Chain Reaction; Tuberculosis

Mice with experimental tuberculosis were given isoniazid, rifampicin, erythromycin, cefotaxime, ofloxacin. Erythromycin, cefotaxime and ofloxacin enhanced macrophage activity if their course did not exceed 2-4 weeks. Isoniazid and rifampicin for a short time inhibited macrophage activity then stimulated it. These findings led the authors to the conclusion that erythromycin, cefotaxime and ofloxacin must be used only in short courses.

Topics: Animals; Anti-Bacterial Agents; Anti-Infective Agents; Antitubercular Agents; Cefotaxime; Cephalosporins; Erythromycin; Isoniazid; Macrophages; Mice; Ofloxacin; Time Factors; Tuberculosis

Cephalosporin antibiotics cefamezin (I generation drug sensitive to beta-lactamase) and cefotaxime (III generation drug resistant to beta-lactamase) have been tested for antituberculous activity. The latter was found dependent on resistance to mycobacterial beta-lactamase. A minimal inhibiting concentration of cefotaxime was similar to that of etambutol and tisamid. Cefotaxime also enhanced tuberculostatic and bactericidal effect of isoniazid and rifampicin. Combination cefotaxime+isoniazid+rifampicin proved more effective than cefotaxime+etambutol+tisamid. The effectiveness against tuberculosis of the beta-lactamase resistant cephalosporin points to the validity of further research for active phthisiatric drugs among III generation cephalosporins.

Topics: Animals; Cefazolin; Cefotaxime; Cells, Cultured; Cephalosporins; Culture Media; Drug Therapy, Combination; Ethambutol; Humans; Isoniazid; Mice; Microbial Sensitivity Tests; Rifampin; Tuberculosis