cdw17-antigen and Carcinoma--Renal-Cell

Lactosylceramide sulfate and galactosylceramide sulfate were found to increase markedly in human renal cell carcinoma (adenocarcinoma) as compared to uninvolved tissue. Activities of two sulfotransferases toward galactosylceramide and lactosylceramide as substrates were significantly elevated in the carcinoma compared to the uninvolved tissue resulting in enhanced synthesis of the two sulfatides in the carcinoma. The elevation of the two sulfotransferases was parallel in most tumors, suggesting that the same enzyme is responsible for the enhanced synthesis of two sulfatides. No consistent difference in the activity of arylsulfatase A, which desulfates the two sulfatides, was observed between the carcinoma and uninvolved tissue. Both the present and previous results show that the increased synthesis of the sulfatide(s) due to elevated sulfotransferase activity could be a biochemical characteristic common to adenocarcinomas derived from different tissues.

Topics: Adenocarcinoma; Adult; Aged; Antigens, CD; Carcinoma, Renal Cell; Cerebroside-Sulfatase; Female; Galactosylceramides; Glycosphingolipids; Humans; Kidney Neoplasms; Lactosylceramides; Male; Middle Aged; Sulfoglycosphingolipids; Sulfurtransferases

We performed immunohistochemical examination of serial sections of human fetal and adult renal tissue as well as human renal carcinoma tissue, using monoclonal antibodies T5A7, 1B2, FH2, FH4, and FH6. These monoclonal antibodies were directed to lacto series type 2 antigens with sugar-chain structures: lactosylceramide, lactoneotetraosylceramide (paragloboside), Lex (a chemically well-defined fucosyl carbohydrate antigen), difucosyl Lex, and sialosyl-difucosyl Lex, respectively. The staining pattern in fetal renal tissue changed significantly according to the stage of organogenesis. In addition, expression of the antigens, especially paragloboside and sialosyl-difucosyl Lex, was closely related to the prognosis of the patient. These results suggest that the expression of a series of oncofetal antigens in development or differentiation of organs is reflected in the reversion to an immature pattern of antigenic expression in tumor tissue. The pattern of antigen expression in renal tumors offers a good criterion for ascertaining the degree of tumor differentiation and malignancy and is valuable for determining prognosis.

Topics: Adult; Antibodies, Monoclonal; Antigens, CD; Antigens, Neoplasm; Carcinoma, Renal Cell; Embryo, Mammalian; Female; Fetus; Globosides; Glycolipids; Glycosphingolipids; Humans; Immunohistochemistry; Kidney; Kidney Neoplasms; Lactosylceramides; Lewis X Antigen; Neoplasm Staging; Pregnancy; Prognosis