cathepsin-g and Hepatitis

Anti-cathepsin G antibodies have been detected by using three different methods. i) Binding to azurophilic granules constituents after separation of purified alpha-granules on Matrex gel Orange A chromatography according to Kao. ii) Binding to azurophilic granules freezed and thawed after coating on ELISA plates. iii) Binding to purified cathepsin G in ELISA assay. Anti-cathepsin G antibodies were observed patient's sera with ulcerative colitis, primary sclerosing cholangitis, primary biliary cirrhosis and autoimmune hepatitis but not in controls or patients with chronic viral hepatitis or vasculitis.

Topics: Antibodies, Antineutrophil Cytoplasmic; Autoantibodies; Autoimmune Diseases; Cathepsin G; Cathepsins; Chromatography, Affinity; Cytoplasmic Granules; Enzyme-Linked Immunosorbent Assay; Freezing; Hepatitis; Hot Temperature; Humans; Immunoglobulin G; Inflammatory Bowel Diseases; Serine Endopeptidases