cassaine and Prostatic-Neoplasms

Cytotoxic activity-guided fractionation of Erythrophleum fordii led to the isolation of two new cassaine diterpenoid-diterpenoid amide dimers, erythrophlesins H-I (1, 2). Spectral data indicated that they consist of asymmetrical dimeric structure via an ester bond between two cassaine diterpenoids. MTT assay confirmed that compound 1, erythrophlesin H, had the strongest cytotoxic effect toward the human prostate cancer cell line, PC-3. The molecular mechanism by which this compound induced apoptosis cell in prostate cancer remains unknown. Erythrophlesin H induced apoptosis in a dose-dependent manner. Acridine orange and annexin V-FITC/PI double staining confirmed that erythrophlesin H effectively induces apoptosis in PC-3 cells.

Topics: Abietanes; Alkaloids; Amides; Antineoplastic Agents, Phytogenic; Apoptosis; Cell Proliferation; Diterpenes; Fabaceae; Humans; In Vitro Techniques; Male; Molecular Structure; Prostatic Neoplasms; Spectrometry, Mass, Electrospray Ionization; Tumor Cells, Cultured