casein-kinase-ii and Calcinosis

Osteopontin is a noncollagenous, phosphorylated extracellular glycoprotein, expressed in mineralized and nonmineralized tissues, organs and body fluids. The protein contains an RGD tripeptide cell-binding motif, and is subjected to a variety of posttranslational modifications that play important roles in its multiple biological functions, such as bone remodeling and inhibition of pathological calcification. In this study, we have expressed bovine osteopontin in a prokaryotic system and identified the seven amino acid residues phosphorylated in vitro by CKII.

Topics: Animals; Bone and Bones; Bone Remodeling; Calcification, Physiologic; Calcinosis; Casein Kinase II; Cattle; Escherichia coli; Extracellular Matrix; Osteogenesis; Osteopontin; Phosphorylation; Protein Processing, Post-Translational; Recombinant Proteins; Trypsin