casein-hydrolysate has been researched along with Insulin-Resistance* in 3 studies
3 other study(ies) available for casein-hydrolysate and Insulin-Resistance
| Article | Year |
|---|---|
Casein Hydrolysate Alleviates Adipose Chronic Inflammation in High Fat-Diet Induced Obese C57BL/6J Mice through MAPK Pathway.
Topics: 3T3-L1 Cells; Animals; Caseins; Cattle; Diet, High-Fat; Inflammation; Insulin Resistance; Mice; Mice, Inbred C57BL; Mice, Obese; Mitogen-Activated Protein Kinases; Obesity; Tumor Necrosis Factor-alpha | 2023 |
Identification of novel peptides from goat milk casein that ameliorate high-glucose-induced insulin resistance in HepG2 cells.
In this study, we investigated the effect of goat milk casein hydrolysates on glucose consumption rate, intracellular glycogen concentration, and mRNA expression of gluconeogenesis-related genes, including phosphoenolpyruvate carboxykinase 1 (PCK1) and glucose-6-phosphatase catalytic subunit (G6PC), in insulin-resistant HepG2 cells. From the obtained hydrolysates, we also purified and characterized novel peptides that ameliorated high-glucose-induced insulin resistance in HepG2 cells. The 3-h hydrolysate caused the highest glucose consumption rate in insulin-resistant HepG2 cells. It also showed positive effects on promoting intracellular glycogenesis and reducing mRNA expression of PCK1 and G6PC. We separated the obtained hydrolysates into 3 fractions (F1, F2, and F3) by gel filtration chromatography; we further purified F1 using reversed-phase HPLC and identified peptides using liquid chromatography-tandem mass spectrometry. The bioactive peptides identified were SDIPNPIGSE (α Topics: Animals; Caseins; Chromatography, High Pressure Liquid; Chromatography, Liquid; Gluconeogenesis; Glucose; Glycogen; Goats; Hep G2 Cells; Humans; Insulin Resistance; Milk; Peptides | 2020 |
A casein hydrolysate protects mice against high fat diet induced hyperglycemia by attenuating NLRP3 inflammasome-mediated inflammation and improving insulin signaling.
Activation of the nod-like receptor protein 3 (NLRP3) inflammasome is required for IL-1β release and is a key component of obesity-induced inflammation and insulin resistance. This study hypothesized that supplementation with a casein hydrolysate (CH) would attenuate NLRP3 inflammasome mediated IL-1β secretion in adipose tissue (AT) and improve obesity-induced insulin resistance.. J774.2 macrophages were LPS primed (10 ng/mL) and stimulated with adenosine triphosphate (5 mM) to assess NLRP3 inflammasome activity. Pretreatment with CH (1 mg/mL; 48 h) reduced caspase-1 activity and decreased IL-1β secretion from J774.2 macrophages in vitro. 3T3-L1 adipocytes cultured with conditioned media from CH-pretreated J774.2 macrophages demonstrated increased phosphorylated (p)AKT expression and improved insulin sensitivity. C57BL/6JOLaHsd mice were fed chow or high fat diet (HFD) for 12 wk ± CH resuspended in water (0.5% w/v). CH supplementation improved glucose tolerance in HFD-fed mice as determined by glucose tolerance test. CH supplementation increased insulin-stimulated pAKT protein levels in AT, liver, and muscle after HFD. Cytokine secretion was measured from AT and isolated bone marrow macrophages cultured ex vivo. CH supplementation attenuated IL-1β, tumor necrosis factor alpha (TNF-α) and IL-6 secretion from AT and IL-1β, IL-18, and TNF-α from bone marrow macrophages following adenosine triphosphate stimulation ex vivo.. This novel CH partially protects mice against obesity-induced hyperglycemia coincident with attenuated IL-1β secretion and improved insulin signaling. Topics: 3T3-L1 Cells; Adipose Tissue; Animals; Caseins; Cytokines; Diabetes Mellitus, Type 2; Diet, High-Fat; Hyperglycemia; Inflammasomes; Inflammation; Insulin; Insulin Resistance; Interleukin-1beta; Interleukin-6; Mice; Mice, Inbred C57BL; NLR Family, Pyrin Domain-Containing 3 Protein; NLR Proteins; Obesity; Tumor Necrosis Factor-alpha | 2016 |